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OfflineLapius
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Potato Dextrose Agar
    #4308913 - 06/17/05 09:59 PM (12 years, 5 months ago)

Acquired some corn syrup. Had a choice of a few. Chose light corn syrup w/ vanilla. Didn't want the vanilla in it, but than, didn't want dark thick corn syrup, purely for contaminate visibility reasons. Was I wrong to get the clear "light" syrup w/ added vanilla? Should I just acquire the basic dark corn syrup?  :confused:

Here is the recipe:
Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)

:mushroom2:


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InvisibleRoadkillM
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Re: Potato Dextrose Agar [Re: Lapius]
    #4309483 - 06/18/05 12:34 AM (12 years, 5 months ago)

I have never tried using corn syrup with mine.

I do use PDYA and MEA.

I follow pretty much the same recipies as are stated in the FAQ.

Copy and Pasted from the FAQ :

Quote:

These recipes are designed for agar maintenance of Psilocybe species. All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure. 500 ml of medium will pour around 20 petri dishes. Using a different medium from time to time will prevent the culture getting used to one medium.

Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water

Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)




If you read the PDYA one there are several different ways you can make it.

This is the one I make.

-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast

I switch back and forth with the above PDYA recipy and the MEA.
And have always gotten great results.

Click here for all the agar recipies that are listed!~

good luck!~




tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleThumpaCap
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Registered: 02/19/05
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Re: Potato Dextrose Agar [Re: Roadkill]
    #4312778 - 06/19/05 02:49 AM (12 years, 5 months ago)

:thumbup:


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Offlinekomsija
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Re: Potato Dextrose Agar [Re: ThumpaCap]
    #10080566 - 03/31/09 04:02 PM (8 years, 7 months ago)

i was making agar plate  with a potato recipe...PDA
i was doing just the same as was writing here on forums...i clone a oyster mushroom....but i get just a litle colonization after 17 days:(.
I dont now what i am doing wrong:(. after 17 days i see colonization , but its not complitly pure white coler/? Is thet normal, what i am doing wrong?


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InvisibleKrazy L
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Re: Potato Dextrose Agar [Re: komsija]
    #10081266 - 03/31/09 05:45 PM (8 years, 7 months ago)

Nice...3 yr old thread.  lol


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Offlinekomsija
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Re: Potato Dextrose Agar [Re: Lapius]
    #10081280 - 03/31/09 05:47 PM (8 years, 7 months ago)

lol...just needed for a help...


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OfflineFreeSporePrints
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Re: Potato Dextrose Agar [Re: komsija]
    #10143557 - 04/10/09 06:16 PM (8 years, 7 months ago)

Be sure your pH isn't too much acid or basic.

Be sure your medium into the petri isn't full of water drops.

Be sure your specimen isn't old.

Be sure your oyster mushroom isn't a commercial mushroom. I'm not saying you can't clone commercial specimen, but a lot of time they come from a strain so much expanded that they neither haven't the power to grow a bit.

Try with others oyster first!

Fabio


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Invisiblefastfred
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Re: Potato Dextrose Agar [Re: FreeSporePrints]
    #10149298 - 04/11/09 10:05 PM (8 years, 7 months ago)

Might want to mention that none of these recipies produce PDA they are all modified PDA recipies.


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OfflineChandlerxgore
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Re: Potato Dextrose Agar [Re: fastfred]
    #15096813 - 09/18/11 06:15 PM (6 years, 2 months ago)

Can you use 1 gram of regular yeast instead of brewer's yeast, yeast extract, or nutritional yeast?

Is there a difference between the yeasts?


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InvisiblePrisoner#1
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Re: Potato Dextrose Agar [Re: Chandlerxgore]
    #15097285 - 09/18/11 08:23 PM (6 years, 2 months ago)

the regular yeast will work but the others are more suited to the task and
typically far cheaper, brewers and nutritional yeast can be found at health
food stores and often in drug stores

BTW... the thread is a couple of years old


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Offline760Rollz
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Re: Potato Dextrose Agar [Re: Prisoner#1]
    #15139382 - 09/27/11 12:25 AM (6 years, 1 month ago)

If this thread is that old I might as well ask my question here.  I am trying to do agar work.  I have made some agar using the recipe RoadKill posted in this thread.  The agar that I make only gels up when I put it in the fridge.  I don't know where I got the idea that agar should turn to gel, but I'm under the impression you want the agar to gel up.  Should it be in a gel state to work with?  I can't find any info on it.


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InvisibleJavadog
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Re: Potato Dextrose Agar [Re: 760Rollz]
    #15146221 - 09/28/11 11:15 AM (6 years, 1 month ago)

Yes, it should be firm at room temperature.

You may need to add more agar powder.

Good luck,

JD


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Myco-tek.org


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