Home | Community | Message Board


High Mountain Compost
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Offlinejohnuk
Strangerlove
Registered: 06/13/05
Posts: 226
Last seen: 7 years, 2 months
?Karo? method for truffle cloning?
    #4308527 - 06/17/05 08:17 PM (12 years, 1 month ago)

I've been having a look round for the things I need to agar clone Mexicana for incolution, but it's not easy to pick up from local stores (I'm in the UK).

I was wondering if anyone had tried the ?Karo? method for cloning truffles?

I'd fill something like a PP container with clean water. Add a few tablespoons of sugar, film top shut. Nuke. Slice open truffle in a glove bag, remove samples from sterile core and drop into sugar solution.

I know there are a small number of spores inside truffles but probably not enough for this to result in growth directly. I'd be looking to have the mycelium clone through the solution. Then inject into bags or jars.

That'd be easier and cleaner than dealing with agar incolulation.

I know I can get hold of agar mediums through the post if I have to but I can do this from things in my kitchen. And I like cooking anyway! :wink:

If no one's had a go with truffles yet has anyone managed to clone normal shoom mycelium using the method?

Thanks guys!
John


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletahoe
Noob Slayer
Male

Registered: 11/27/03
Posts: 6,216
Loc: N38.93829W119.98108
Trusted Cultivator
Re: ?Karo? method for truffle cloning? [Re: johnuk]
    #4308689 - 06/17/05 08:59 PM (12 years, 1 month ago)

it would work if you can get a clean specimen. The chunk of truffle, sclerotia, truffles are edibles, will go back to a rhizomorphic state and consume the liquid. use this to inoc more seed. This lc will be prone to form sclerotia, it wont form rocks in liquid


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShdwstr
FSRCanada
Male

Registered: 02/18/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 3 years, 6 months
Re: ?Karo? method for truffle cloning? [Re: tahoe]
    #4309610 - 06/18/05 01:12 AM (12 years, 1 month ago)

Quote:

tahoe said:
This lc will be prone to form sclerotia, it won't form rocks in liquid




Most of what you said made sense... except this... please elaborate!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshirley knott
not my real name
 User Gallery
Registered: 11/11/02
Posts: 9,105
Loc: London Flag
Last seen: 6 months, 13 days
Trusted Cultivator
Re: ?Karo? method for truffle cloning? [Re: johnuk]
    #4310193 - 06/18/05 06:42 AM (12 years, 1 month ago)

by 'truffle' you seem to be talking about ps mexicana sclerotia (which can be legally purchased in London shops still). to grow from these, either dunk in peroxide and then place on peroxidated agar, or clone a sterile piece from the middle and inoculate lc from there. sterile tissue to lc works fine, as does spores to lc, agar-myc to lc, and even sterile colonised substrate to lc.

wrong forum though - this is for gourmet mushrooms, the other kind of truffle.
i'd move it if i could, but i can't so i won't.


--------------------
buh


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* karo method impalaelton 364 2 03/09/05 03:42 AM
by LethalX5
* Cloning sclerotia? Submob 3,847 9 12/20/03 09:00 PM
by OJK
* What is the best way to clone sclerotia hottamali 4,151 8 06/13/08 06:41 AM
by Boarders
* FIRST GROW! Using the Karo method. Leviathan 751 9 03/05/08 09:15 AM
by Leviathan
* Mexicana Truffles Dragoncaviar 2,815 13 10/09/16 01:58 AM
by Turquoise buttons
* Psilocybe tampanensis sclerotia --- ? Tlaloc 4,485 11 11/19/01 04:31 PM
by Doomhammer
* Atlantis sclerotia question roquet 1,828 8 09/09/07 08:32 PM
by roquet
* karo water/cloning GregHimself 475 1 08/16/03 03:52 AM
by deanofmean

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,203 topic views. 36 members, 133 guests and 21 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
SoulSpeciosa Kratom
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.004 seconds on 19 queries.