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Offlinejohnuk
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Registered: 06/13/05
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?Karo? method for truffle cloning?
    #4308527 - 06/17/05 08:17 PM (13 years, 7 days ago)

I've been having a look round for the things I need to agar clone Mexicana for incolution, but it's not easy to pick up from local stores (I'm in the UK).

I was wondering if anyone had tried the ?Karo? method for cloning truffles?

I'd fill something like a PP container with clean water. Add a few tablespoons of sugar, film top shut. Nuke. Slice open truffle in a glove bag, remove samples from sterile core and drop into sugar solution.

I know there are a small number of spores inside truffles but probably not enough for this to result in growth directly. I'd be looking to have the mycelium clone through the solution. Then inject into bags or jars.

That'd be easier and cleaner than dealing with agar incolulation.

I know I can get hold of agar mediums through the post if I have to but I can do this from things in my kitchen. And I like cooking anyway! :wink:

If no one's had a go with truffles yet has anyone managed to clone normal shoom mycelium using the method?

Thanks guys!
John


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Invisibletahoe
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Re: ?Karo? method for truffle cloning? [Re: johnuk]
    #4308689 - 06/17/05 08:59 PM (13 years, 7 days ago)

it would work if you can get a clean specimen. The chunk of truffle, sclerotia, truffles are edibles, will go back to a rhizomorphic state and consume the liquid. use this to inoc more seed. This lc will be prone to form sclerotia, it wont form rocks in liquid


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OfflineShdwstr
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Registered: 02/18/01
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Re: ?Karo? method for truffle cloning? [Re: tahoe]
    #4309610 - 06/18/05 01:12 AM (13 years, 6 days ago)

Quote:

tahoe said:
This lc will be prone to form sclerotia, it won't form rocks in liquid




Most of what you said made sense... except this... please elaborate!


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Offlineshirley knott
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Re: ?Karo? method for truffle cloning? [Re: johnuk]
    #4310193 - 06/18/05 06:42 AM (13 years, 6 days ago)

by 'truffle' you seem to be talking about ps mexicana sclerotia (which can be legally purchased in London shops still). to grow from these, either dunk in peroxide and then place on peroxidated agar, or clone a sterile piece from the middle and inoculate lc from there. sterile tissue to lc works fine, as does spores to lc, agar-myc to lc, and even sterile colonised substrate to lc.

wrong forum though - this is for gourmet mushrooms, the other kind of truffle.
i'd move it if i could, but i can't so i won't.


--------------------
buh


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