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OfflineGatorB
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Registered: 07/30/04
Posts: 233
Loc: Southeast Tennessee
Last seen: 10 years, 8 months
Why are my LCs growing so slowly?
    #4295622 - 06/14/05 06:32 PM (12 years, 1 month ago)

I followed agar's little baby bottle tek with the reverse nipple, but my myc is taking FOREVER to colonize and fill the bottle. Do I need to inject sterile air with a syringe or something?


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Invisibleagar
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Registered: 11/21/04
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295654 - 06/14/05 06:39 PM (12 years, 1 month ago)

If you can see a cloud of Myc in it, it is good to go.

Seldom - if ever will myc colonize all the solution.

A picture would help.


--------------------


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OfflineGatorB
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Re: Why are my LCs growing so slowly? [Re: agar]
    #4295727 - 06/14/05 06:55 PM (12 years, 1 month ago)

Here are some pics:



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OfflineGatorB
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Registered: 07/30/04
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295730 - 06/14/05 06:55 PM (12 years, 1 month ago)

Is that about all the colonization I can expect? Just shake up and use broken up myc in my syringe?


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Invisibleagar
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295741 - 06/14/05 06:58 PM (12 years, 1 month ago)

You should have a lot more colonization than that.
What nute did you use?


--------------------


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Offlinethecazz723
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Registered: 04/14/05
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295777 - 06/14/05 07:04 PM (12 years, 1 month ago)

To increase the amount of mycelium in a LC, I add 5-10ml (per 200ml culture) of supernatant bean liquid. That is: Alcohol wipe a can opener (The kind that makes triangle holes not the 'round about kind) and a can of beans. Then make a hole and suck 5-10ml of the liquid out of the can. The preservatives get diluted and growth increases tremendously IME.


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OfflineGatorB
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Re: Why are my LCs growing so slowly? [Re: thecazz723]
    #4295786 - 06/14/05 07:06 PM (12 years, 1 month ago)

i used karo. jars were knocked up like 3-4 weeks ago.


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InvisibleRoadkill
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295797 - 06/14/05 07:09 PM (12 years, 1 month ago)

how much Karo did you use?

how much water did you use?

how many cc's of a spore syringe did you use?

what temp are you incubating these at?

what color is the Moon? <---lol

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineGatorB
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Re: Why are my LCs growing so slowly? [Re: Roadkill]
    #4295807 - 06/14/05 07:12 PM (12 years, 1 month ago)

karo = less than 3% of the mixture as commanded by agar

water = see above

spores = 3 cc's each bottle

temps = 84-88F


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295838 - 06/14/05 07:20 PM (12 years, 1 month ago)

I'd try more like 5% of karo...but thats just me...I get more growth then you, but still not perfect...


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleRoadkill
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295846 - 06/14/05 07:22 PM (12 years, 1 month ago)

and what color is the Moon?

lolzz

---

I use Tall half pint jars for LC's.

4cc's of light Karo.
96cc's of water.
1 marble.

just double up the recipe for larger jars.

Sterilize for 30 minutes and let it cool.

I inject 1 to 2cc's of spores.

the results...


I use this kind of lid now...self sealing injection port...with a round bandaid over one hole for a filter disc.


I think part of the problem is there is no air exchange...with the baby bottle tek.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleRoadkill
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295855 - 06/14/05 07:24 PM (12 years, 1 month ago)

Quote:

GatorB said:


temps = 84-88F




temps are too high!~

81 to 84 degrees F.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineGatorB
Stranger
Registered: 07/30/04
Posts: 233
Loc: Southeast Tennessee
Last seen: 10 years, 8 months
Re: Why are my LCs growing so slowly? [Re: Roadkill]
    #4295902 - 06/14/05 07:34 PM (12 years, 1 month ago)

Is there a good tek to follow with 1/2 pint jars? I haven't been able to seem to find anything.


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Offlinescatmanrav
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Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295912 - 06/14/05 07:35 PM (12 years, 1 month ago)

Quote:

GatorB said:
Is there a good tek to follow with 1/2 pint jars? I haven't been able to seem to find anything.




Whats wrong with:

"4cc's of light Karo.
96cc's of water.
1 marble.

just double up the recipe for larger jars.

Sterilize for 30 minutes and let it cool.

I inject 1 to 2cc's of spores."


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Why are my LCs growing so slowly? [Re: GatorB]
    #4295923 - 06/14/05 07:36 PM (12 years, 1 month ago)

Quote:

GatorB said:

Is there a good tek to follow with 1/2 pint jars?  I haven't been able to seem to find anything.




I just gave it to you.

read my post.

:smirk:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineKalix
'Head

Registered: 03/20/05
Posts: 1,504
Last seen: 11 years, 7 months
Re: Why are my LCs growing so slowly? [Re: Roadkill]
    #4295936 - 06/14/05 07:38 PM (12 years, 1 month ago)

Quote:

Roadkill said:
and what color is the Moon?

lolzz

---


tc






--------------------


My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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Offlinexburn
V card
Registered: 03/10/05
Posts: 707
Last seen: 5 years, 12 days
Re: Why are my LCs growing so slowly? [Re: Roadkill]
    #4295937 - 06/14/05 07:38 PM (12 years, 1 month ago)

its blue and made of cheese


--------------------
Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.


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OfflineKalix
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Re: Why are my LCs growing so slowly? [Re: xburn]
    #4295944 - 06/14/05 07:39 PM (12 years, 1 month ago)

I always figured RK didn't go outside enuff


--------------------


My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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InvisibleRoadkill
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Posts: 22,598
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Re: Why are my LCs growing so slowly? [Re: Kalix]
    #4295969 - 06/14/05 07:43 PM (12 years, 1 month ago)

it was sunny today...and I went to the beach.

it started raining on us 30 minutes after we got there.

:frown:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKalix
'Head

Registered: 03/20/05
Posts: 1,504
Last seen: 11 years, 7 months
Re: Why are my LCs growing so slowly? [Re: Roadkill]
    #4295982 - 06/14/05 07:45 PM (12 years, 1 month ago)

Shitty.. It rained on and off all day here in the heartland.


--------------------


My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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Amazon Shop: ½ Pint Jars, Agar

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