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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflineForbiddenFruit
DaShroomGuy
Registered: 03/09/05
Posts: 17
Last seen: 11 years, 9 months
What am i doing wrong here?
    #4294667 - 06/14/05 12:20 PM (13 years, 7 days ago)

Alright, I steam sterilized a jar (1 pint) for 45 minutes. It was BRF/verm substrate. And about 3 days later, there was this nasty sour smell coming from it, so I identified it as contam, pitched it's contents. Is 45 mins for one jar not enough? Oh, and I don't have a PC, so I have to work with what I have atm, until I get one.


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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
Posts: 4,207
Last seen: 12 years, 8 months
Re: What am i doing wrong here? [Re: ForbiddenFruit]
    #4294672 - 06/14/05 12:24 PM (13 years, 7 days ago)

Steam is not enough to sterilize bacterial endospores. If I were you and I couldn't use a PC I'd steam the jars like you did every day for 3 days straight.

That should allow the bacterial spores to germinate and thus become possible to kill with 212 degrees.


--------------------
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Smoke plenty of green, and eat fungus!!!!
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Invisiblefufungi
Stranger
Male

Folding@home Statistics
Registered: 03/16/05
Posts: 224
Re: What am i doing wrong here? [Re: ForbiddenFruit]
    #4294815 - 06/14/05 01:39 PM (13 years, 7 days ago)

My friend has been steaming jars and so far has 0 contams out of 11 jars. Four of those are in the pmp. 7 more are about 20% colonized. He boils them for 90 minutes, so I would say increase your steam time and you'll be ok. :cool:


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InvisibleRoadkillM
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: What am i doing wrong here? [Re: ForbiddenFruit]
    #4295128 - 06/14/05 04:32 PM (13 years, 7 days ago)

45 minutes of steaming for a Pint jar of BRF/Verm isn't enough.

try 1 1/2 hours for a Pint jar.

---

Smallworld,

Quote:

Steam is not enough to sterilize bacterial endospores.




there is no endospores in BRF.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 9 years, 2 months
Re: What am i doing wrong here? [Re: Roadkill]
    #4295154 - 06/14/05 04:36 PM (13 years, 7 days ago)

Quote:

Roadkill said:

there is no endospores in BRF.




--------------------


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OfflineKalix
'Head

Registered: 03/20/05
Posts: 1,504
Last seen: 12 years, 6 months
Re: What am i doing wrong here? [Re: LengthyFellow]
    #4295232 - 06/14/05 05:08 PM (13 years, 7 days ago)

Yea, when I did PFTek I steamed for 2 hrs, and lost 50% to bacteria anyway. There's no getting around how awesome it is to have a PC...


Edit: PS, I think my incubator being too warm had a lot to do with it too..


--------------------


My Unitarian Jihad Name is: The Shotgun of Sweet Reason


Edited by Kalix (06/14/05 05:10 PM)


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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