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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood, Wild Bird Seed

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OfflineSmallworlds
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Registered: 03/12/05
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Success!! (There is a question in here too)
    #4294664 - 06/14/05 12:18 PM (12 years, 5 months ago)

9 of 9 jars with no contams using a stupid U-100 insulin syringe and the oven tek for syringe making, and Doc34's WBS tek for the jar preperation!

(That's right Anno, the oven tek produced 100% success!)


Incidentally, I like using the U-100 insulin syringes the needle makes a much, much smaller hole for possible contams to enter. I just made sure the spore concentration was high. Worked like a charm.

My question is this: How do you judge when the jars are 100% colonized? There are 6 out of 9 that have growth covering every visible area, (the other 3 are fine but progressing at varying slower rates.) but I'm sure you're supposed to wait longer than that to make sure the centers are done right?

They are 10 days old now. Give me your best ideas on how to tell when the jars are actually 100% colonized please.


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


Trip Report


Edited by Smallworlds (06/14/05 12:46 PM)


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Offlinesublime40oz
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Re: Success!! (There is a question in here too) [Re: Smallworlds]
    #4294777 - 06/14/05 01:16 PM (12 years, 5 months ago)

Another 4 days or so after the outside is colonized, seems to be a rough rule of thumb.


--------------------
Beyond the gray sky


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OfflineSmallworlds
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Registered: 03/12/05
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Re: Success!! (There is a question in here too) [Re: sublime40oz]
    #4294783 - 06/14/05 01:21 PM (12 years, 5 months ago)

Sounds great man, they can be cased this weekend then, thanks a lot!


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


Trip Report


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OfflineAnnoA
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Re: Success!! (There is a question in here too) [Re: Smallworlds]
    #4294798 - 06/14/05 01:27 PM (12 years, 5 months ago)

>That's right Anno, the oven tek produced 100% success!

How do you know this? Did you make another set of syringes at the same time in your kitchen WITHOUT the use of the oven? No? So how do you know the oven helped in any way?


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OfflineSmallworlds
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Re: Success!! (There is a question in here too) [Re: Anno]
    #4294817 - 06/14/05 01:39 PM (12 years, 5 months ago)

In a sterile process any weak link in the chain should produce contaminations. The simple fact that no contaminations occured indicates to me that the oven tek is an O.K. process to use.

If I would have gotten contams it most likely would have been from either:

-Contaminated sporeprint
-Bad syringe making technique
-Contams entering during inoculation

And then it would be easy to blame the oven tek for it.

The fact that I did not get any contams means that all three steps were done correctly, and that all of the techniques used actually do work.

I use the process of elimination to deduce that the oven tek works. :shrug:


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


Trip Report


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OfflineAnnoA
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Re: Success!! (There is a question in here too) [Re: Smallworlds]
    #4294831 - 06/14/05 01:45 PM (12 years, 5 months ago)

Your deduction is flawed.


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OfflineWojo
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Registered: 04/02/05
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Re: Success!! (There is a question in here too) [Re: Anno]
    #4294857 - 06/14/05 01:58 PM (12 years, 5 months ago)

Other people have had absolutely no success with the oven tek (me for example)


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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
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Re: Success!! (There is a question in here too) [Re: Anno]
    #4294862 - 06/14/05 02:00 PM (12 years, 5 months ago)

I can see why you say that, but I think the flaw is very small.

Rather than conduct tests using a control group, I'm going to continue using what I have seen work, and then after 10 or so times we can review how the oven tek has treated me.


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


Trip Report


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OfflineAnnoA
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Re: Success!! (There is a question in here too) [Re: Smallworlds]
    #4294868 - 06/14/05 02:03 PM (12 years, 5 months ago)

>but I think the flaw is very small.

You can think what you want, but you won't KNOW until you do a counter test.

>Rather than conduct tests using a control group, I'm going to continue using what I have seen work

Whatever, you obviously don't want to realize that what you are doing doesn't help the collective knowledge one bit.


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OfflineSmallworlds
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Re: Success!! (There is a question in here too) [Re: Wojo]
    #4294884 - 06/14/05 02:17 PM (12 years, 5 months ago)

Quote:

Wojo said:
Other people have had absolutely no success with the oven tek (me for example)




It couldn't have been a bad print or some other reason?

I'm not trying to set anything in stone here, but I am merely happy to report a 100% successful experience in which the oven tek was a part of the process.

Here's how it went:

The oven was sterilized at 350 for 45 minutes, including a work tray. a shotglass was sterilized in a baggie with a capful of isopropyl 70% alcohol in it. a syringe was presterilized with boiling water and left filled with sterile water to cool.

The oven was turned off and the oven thermometer watched until it had cooled to 250 degrees. Then the oven door was opened, the shotglass was removed from the bag and placed on a makeshift coaster made from a coffee filter to prevent breakage, on the worktray. A sterile print made by microwaving index cards first was opened and the needle sterilized with alcohol. The needle was used to scrape spores into the shotglass, and then stirred around. The sporewater was then sucked-up into the syringe. The entire syringe making process once the sporesprint was opened was allowed just 20 seconds to complete, minimizing exposure.

The innoculations were performed as indicated in doc34's tek.


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


Trip Report


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Invisibleagar
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Re: Success!! (There is a question in here too) [Re: Smallworlds]
    #4295068 - 06/14/05 04:20 PM (12 years, 5 months ago)


Day 5


What a 100% colonized WBS jar looks like.
As IN when everything in it is completly colonized.

BTW, Oven TEK is flawed.
I get good results on an open air kitchen counter, if you know how.
I get better results on a sanitized bathroon counter, in still air.
You were either lucky, or used a good sterile procedure that kept the needle from being exposed to open air (usualy wrapped in an alc swab).

A good Glove Box, beats OVEN TEK hands down.


--------------------


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InvisibleRoadkillM
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Re: Success!! (There is a question in here too) [Re: agar]
    #4295090 - 06/14/05 04:25 PM (12 years, 5 months ago)

Quote:

agar said:

BTW, Oven TEK is flawed.
I get good results on an open air kitchen counter, if you know how.
I get better results on a sanitized bathroon counter, in still air.
You were either lucky, or used a good sterile procedure that kept the needle from being exposed to open air (usualy wrapped in an alc swab).

A good Glove Box, beats OVEN TEK hands down.





Agree with everything you stated!~

and a good glove box beats everything... but a flowhood.

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleLouiseLouise
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Re: Success!! (There is a question in here too) [Re: agar]
    #4295265 - 06/14/05 05:22 PM (12 years, 5 months ago)

I agree with that :thumbup:
A tech that you did suggests that a heppa is prefered, but...
That's where I started. This time around, I started with 1 syr. of cam. (made in a lab) and one print of OI on foil. 6 of 6 (the last two that barely made it to 95 % went to the great outdoors [so they weren't totally lost])
The IO syr. that I made with no heppa, as agar suggests, is about to fruit now. :mushroom2:
Two different cooks, but done in exactly the same manner.

And, nothing tops a flow hood or glove box :stoned:


--------------------
"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC


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Invisibleohmatic
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Re: Success!! (There is a question in here too) [Re: LouiseLouise]
    #4295603 - 06/14/05 06:29 PM (12 years, 5 months ago)

you know, i have so far spent 1.5 years of cultivation without any aseptical
ibstruments like a flowhood or even a santized glovebox and have sectored and
multilied of chosen genetics of various kinds of fungi (not only cubensis)
on peroxided agar.

only dowbnside is, you cant work off spores unless you got a sterlie master transfer
of some spore mycelium.

anyway, im drunk :wink:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Success!! (There is a question in here too) [Re: ohmatic]
    #4295745 - 06/14/05 06:58 PM (12 years, 5 months ago)

>In a sterile process any weak link in the chain should produce contaminations.

Correct, but turning off the oven, and doing it on your kitchen counter, would not have been a weak link either, because your kitchen was clean enough. If your kitchen werent clean enough, youd have your weak link and youd get contams, with the oven off, or on with you sitting in front of it.

I do all my work open air in the bathroom, always have. Set it right on the back of the toilet and do my agar work open air, but I clean everything first. No air flow, no oven...just a clean area.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisibleohmatic
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Re: Success!! (There is a question in here too) [Re: scatmanrav]
    #4295763 - 06/14/05 07:01 PM (12 years, 5 months ago)

Quote:

scatmanrav said:
If your kitchen werent clean enough, youd have your weak link and youd get contams, with the oven off, or on with you sitting in front of it.





werd :sun:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleRoadkillM
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Re: Success!! (There is a question in here too) [Re: scatmanrav]
    #4295775 - 06/14/05 07:03 PM (12 years, 5 months ago)

I used to do all my work in open air...in my clean kitchen.
my kitchen was spotless...without any air movement of course.

and had very little contams.

---

I have seen one of my friends work in a dirty ass kitchen in open air and get great results...
and I'd have never guessed that it was possible!~
I wouldn't have believed it...if I hadn't seen it with my own eyes.

lolzz


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
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Re: Success!! (There is a question in here too) [Re: Roadkill]
    #4296874 - 06/14/05 11:18 PM (12 years, 5 months ago)

O.K. guys, fine. I am stepping up to a glove box anyway soon, I just wanted to tell everyone the good news, and how it was achieved this time.


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


Trip Report


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OfflineBlue Helix
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Re: Success!! (There is a question in here too) [Re: Smallworlds]
    #4297130 - 06/15/05 12:07 AM (12 years, 5 months ago)

I had great luck in a bathroom using Oust liberally and letting it settle before getting going. Works like a charm and doesn't stink the place up like Lysol does.


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OfflineIGnosticAbhorI
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Re: Success!! (There is a question in here too) [Re: Blue Helix]
    #4297257 - 06/15/05 12:38 AM (12 years, 5 months ago)

Bathroom is probably the gross-est place...in my house lol...

I def. do it either in the kitcken or outside...

Well...use to...got a glove box now :grin:

I tried the oven tek, didn't work out for me...Glad it did for you :thumbup:

-Gnostic


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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood, Wild Bird Seed

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