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Offlinemk31762
Stranger

Registered: 10/18/01
Posts: 14
Last seen: 14 years, 10 months
Lag between sterilization and innoculation
    #429355 - 10/18/01 02:52 PM (15 years, 3 months ago)

Hello all,

This is my first post. I am also trying to cultivate PCs for the first time. My question is, due to time constraints I may have to setup my jars and sterilize them a few days before innoculating them. Does anyone know of a reason why this would be a problem. I am afraid I might be running the risk of contamination.

Thanks.


--------------------
...a leaf of grass is no less than the journeywork
of the stars... Walt Whitman


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OfflineCaseXX
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Registered: 09/14/01
Posts: 123
Last seen: 14 years, 9 months
Re: Lag between sterilization and innoculation [Re: mk31762]
    #429367 - 10/18/01 03:05 PM (15 years, 3 months ago)

I had a similar probelm as you do. My jars sat for almost over a week in a rubbermaid container before I had the chance to inoculate them. Now all 12 of my jars are going strong, I think two more days and I will be ready to case.
CaseXX


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CaseXX
"Dirty Deeds Done Dirt Cheap" AC/DC


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OfflineNecron99
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Registered: 09/08/01
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Re: Lag between sterilization and innoculation [Re: mk31762]
    #429454 - 10/18/01 04:48 PM (15 years, 3 months ago)

At the least you'll get to see if the jars are contaminated before you inocc. Ive heard you should let them sit for 2 or 3 days to see if they contam, and I've heard that you should inocc as soon as they're cool. I PC my jars at night and the next morning it's ready to go.


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"Puny earthlings do not yet know the meaning of the word suffering!!"



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Invisiblerazer
enthusiast
Registered: 10/07/01
Posts: 316
Re: Lag between sterilization and innoculation [Re: CaseXX]
    #429467 - 10/18/01 05:05 PM (15 years, 3 months ago)

Acutally, some suggest to do this (for first timers) if the jars contam then it is obvious that your sterilization techniques need improving. If after a week they are still fine, then go ahead and innoculate.
If they contam after that, you know hte contam was introduced post inoculation.


--------------------
All events stated by myself are only fictionous stories, any relations to true events are intirely coincidental.


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Invisiblerazer
enthusiast
Registered: 10/07/01
Posts: 316
Re: Lag between sterilization and innoculation [Re: razer]
    #429469 - 10/18/01 05:08 PM (15 years, 3 months ago)

Interesting... appears it took me 27+ minutes to pos that reply...


--------------------
All events stated by myself are only fictionous stories, any relations to true events are intirely coincidental.


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Offlinemk31762
Stranger

Registered: 10/18/01
Posts: 14
Last seen: 14 years, 10 months
Re: Lag between sterilization and innoculation [Re: mk31762]
    #429705 - 10/18/01 08:49 PM (15 years, 3 months ago)

Perfect! My jars are hibernating now. Thanks everyone.


--------------------
...a leaf of grass is no less than the journeywork
of the stars... Walt Whitman


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OfflineJammer
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Registered: 11/05/00
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Re: Lag between sterilization and innoculation [Re: mk31762]
    #430128 - 10/19/01 04:23 AM (15 years, 3 months ago)

I just wanted to say that I agree with these opinions. I read somewhere that common mold (the single biggest threat to other compeating fungus is the green mold (penicelin) spore and it needs a good 48 hours in dark/moist enviroments to become active. If this is taken as fact, a wait of a good three days should be a good indication of bad sterlization techniques of the substrat/jars.


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>>Jammer>>


Edited by Jammer (10/19/01 04:26 AM)


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