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drugsaregood
PhD B+
Registered: 04/03/05
Posts: 304
Last seen: 18 years, 8 months
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Bad Karma
#4292446 - 06/13/05 05:36 PM (18 years, 9 months ago) |
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It'll satisfy a bunch of you to hear this. I tried making my own spore syringe last week. I inoculated 12 jars of rye and WBS (6 each). They are all contaminated. It was my first attempt with my own syringe so I'm not surprised.
-------------------- For "incoherent dribble" (drivel) try www.dictionary.com Everybody seems to think I'm lazy I don't mind, I think they're crazy Running everywhere at such a speed Till they find there's no need
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IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,899
Last seen: 10 months, 2 days
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Post deleted by IGnosticAbhorIReason for deletion: Guess
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drugsaregood
PhD B+
Registered: 04/03/05
Posts: 304
Last seen: 18 years, 8 months
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Sterilized pin through a fresh cap suspended over sterilized water in a 1/2 pint jar with a small whole in the lid. Covered with tyvek so I could insert the syringe and suck out the spores/water that dropped. It got the normal green in big blotches but also some strange light green or yellowish around the edges of the grains. I'm colorblind so identifying contaminants is rough. No flow hood last week so that's probably my problem, but now I built a nice one and hopefully can get my agar plates to go.
PS - Gnostic, I wouldn't expect anything positive to come from this post so don't worry about not being helpful.
-------------------- For "incoherent dribble" (drivel) try www.dictionary.com Everybody seems to think I'm lazy I don't mind, I think they're crazy Running everywhere at such a speed Till they find there's no need
Edited by drugsaregood (06/13/05 06:14 PM)
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IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,899
Last seen: 10 months, 2 days
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Hm...That blows.
Glove boxes own.
My gloves arent even attached...And yet My results are about 80% better than in open air
Once I get a bigger on I'll probaby glue the gloves on ect ect....But that'd be for a flow hood
-Gnostic
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Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 3 months
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Duct tape garbage bags to dish gloves, duct tape the other end of the garbage bags too Glove box.. Takes 5min, costs like $1.80
-------------------- My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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drugsaregood
PhD B+
Registered: 04/03/05
Posts: 304
Last seen: 18 years, 8 months
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$29 for 2 blowers, 2 Hepa Filters, 2 furnace filters and a sterilite tub. And a shitload of duct tape
-------------------- For "incoherent dribble" (drivel) try www.dictionary.com Everybody seems to think I'm lazy I don't mind, I think they're crazy Running everywhere at such a speed Till they find there's no need
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drugsaregood
PhD B+
Registered: 04/03/05
Posts: 304
Last seen: 18 years, 8 months
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I'll post a picture eventually once it's actually been successful... I'd hate to post it and then say "it sucks" later on.
-------------------- For "incoherent dribble" (drivel) try www.dictionary.com Everybody seems to think I'm lazy I don't mind, I think they're crazy Running everywhere at such a speed Till they find there's no need
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SouthPArk
C'est La Vie
Registered: 05/11/05
Posts: 740
Loc: SouthPArk
Last seen: 11 years, 7 months
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I dont wanna be an ASS but..
I did a few Prints along with MAking the Syringe to Inculating and have had a 100% success rate..
So even the LAst Time I half assed Every job and Still 0 Contams!!
You need to Spray the SHIT outta your ROOM with LYSOL and use Plenty of Rubbing Alchohal!
I used old syrnges -sucked up boiling water -let cool 15 min in freezer -poured into a 1/2 jar with the already droppped print. -swirled it around and with no luck started to scrap it with my finger YES MY FINGER and well sum stayed on me finger but well it did the job...
-sucked up sum syringes and well WALLAH
im not sure what went wrong for ya but seems like it's a particular thing....
IT could have been a perfectly good Syringe but the Grains were not properly sterilized or sumthn...
do you drool??
Sometime's that shit can get away from ya!!!
-------------------- Live as if you were to die tomorrow. Learn as if you were to live forever.
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drugsaregood
PhD B+
Registered: 04/03/05
Posts: 304
Last seen: 18 years, 8 months
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I'm definitely not the cleanest. Nappy hair, trashy apartment, pets, smoking. There could be anything, but I do have the pressure cooker down pat. The finger in the spore mix kinda makes me wonder what I could have done so wrong. I ran out of spore solution so there are 5 un-inoculated jars that are totally contaminant free. I'm gonna add some of my agar to them and see what happens. I think I read that "pinned cap over a jar of water" tek somewhere on shroomery, but maybe I'm just making shit up in my mind.
-------------------- For "incoherent dribble" (drivel) try www.dictionary.com Everybody seems to think I'm lazy I don't mind, I think they're crazy Running everywhere at such a speed Till they find there's no need
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