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OfflineGruneBrille
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Spores from dried caps?
    #4291487 - 06/13/05 04:04 PM (12 years, 1 month ago)

I was wondering if it were possible to retreive any spores from a dried cap? or will the mushroom have already released all spores by this point. What if numerous dried caps were ground and the entire powdered solution added to a sterile substrate, would there be any worthwile results?


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InvisibleRoadkill
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Re: Spores from dried caps? [Re: GruneBrille]
    #4291501 - 06/13/05 04:08 PM (12 years, 1 month ago)

you might be able to get some growth off of some anti-bacterial agar.

but I wouldn't count on it...

there is a high risk of failure!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinepcubmycol
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Re: Spores from dried caps? [Re: Roadkill]
    #4291520 - 06/13/05 04:12 PM (12 years, 1 month ago)

Quote:

Roadkill said:
you might be able to get some growth off of some anti-bacterial agar.

but I wouldn't count on it...

there is a high risk of failure!~




:thumbup: :thumbup:

You'll likely lose that idea to contaminants with all that dried mush in there.

1. when the mush get wet again, they may rot.
2. that mush is more than likely non-sterile and you couldnt sterilize it without killing the spores, therefore defeating the whole purpose of the experiemnt.


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Offlinedrugsaregood
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Re: Spores from dried caps? [Re: pcubmycol]
    #4292530 - 06/13/05 07:59 PM (12 years, 1 month ago)

I have several agar plates with major rhizo growth from a crushed cap that was black with spores from a shroom above it in the chamber. I used MEA agar without antibacterial or peroxide, there is some green on a couple dishes, but you should be able to isolate a good strain.

:edit - 1 plate got massive black mold which is badddd, a couple others had spots of green, and 10ish are totally white. Is cobweb possible on agar? Never seen cobweb so I'm skeptical of how well my plates grew.


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Edited by drugsaregood (06/13/05 08:02 PM)


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OfflineKalix
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Re: Spores from dried caps? [Re: GruneBrille]
    #4292556 - 06/13/05 08:06 PM (12 years, 1 month ago)

Quote:

GruneBrille said:
I was wondering if it were possible to retreive any spores from a dried cap? or will the mushroom have already released all spores by this point. What if numerous dried caps were ground and the entire powdered solution added to a sterile substrate, would there be any worthwile results?




You'd be better off taking a tissue sample from the inside of a stem, and trying to clone it to agar..


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My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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OfflineKalix
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Re: Spores from dried caps? [Re: Kalix]
    #4292561 - 06/13/05 08:07 PM (12 years, 1 month ago)

And there's always the FSRC 1$ sporeprints, just make sure you send an extra $ or two for a donation, and/or spore prints, when you get 'em..


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My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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OfflineHotnuts
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Re: Spores from dried caps? [Re: Kalix]
    #4293703 - 06/14/05 01:27 AM (12 years, 1 month ago)

Yes. You can easily use dry mushroom spores for renewing your strain. But you must go to a sterile H20 jar and then to a syringe to rehydrate for 24 hours. Just like you would make syringe. Then go to MEA or PDA in a petri dish. The spores will 9 outa 10 times be dirty with mold. Simply put a small dash of solution in the center of a petri, around a 1/10 of a cc. When you get good growth from mushroom mycelium, simply transfer the furthest point of good mycelium from the point of inoculation to another plate to chase out the contaminate. If the contaminate shows up, do the transfer of good clean mycelium from the furthest point from that inoculation point to another new plate.


Edited by hotnutz (06/14/05 01:29 AM)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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