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Offlinegaiajankri
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Registered: 02/06/05
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cultivation of spores on agar
    #4290604 - 06/13/05 12:03 PM (11 years, 7 months ago)

will spores applied to an agar medium from a syrenge germinate in the same manner as when applying dry spores using an inoculation loop?
Namaste


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OfflineAnnoA
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Re: cultivation of spores on agar [Re: gaiajankri]
    #4290612 - 06/13/05 12:05 PM (11 years, 7 months ago)

yes


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Invisibleohmatic
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Re: cultivation of spores on agar [Re: Anno]
    #4290954 - 06/13/05 01:28 PM (11 years, 7 months ago)

iho they will even do better as when being stoken on the agar id you do it the first time.

i myself noticed that it can be quite difficult for one doing it for the first time,
to pick up spores from the print and streaking them properly on the agars surface.

if you put a drop of spore solution onto the dish's surface, you can even move the
dish around after sealing it and spreading the sporesolution therefor
multiplying germination points.

:laugh:
btw, agar rocks, tissue cultures are great fun :smile:

peace ohm :mushroom2:


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Offlinepcubmycol
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Re: cultivation of spores on agar [Re: ohmatic]
    #4291099 - 06/13/05 02:04 PM (11 years, 7 months ago)

post deleted


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Edited by pcubmycol (06/13/05 02:06 PM)


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Offline5meopsyco
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Re: cultivation of spores on agar [Re: pcubmycol]
    #4291195 - 06/13/05 02:50 PM (11 years, 7 months ago)

make sure you only use a very small amount...


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Offlinexburn
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Re: cultivation of spores on agar [Re: 5meopsyco]
    #4291374 - 06/13/05 03:40 PM (11 years, 7 months ago)

i drop is all that is needed. leave it in the center so it grows out evenly and you have a better chance of quick isolation


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OfflineBlue Helix
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Re: cultivation of spores on agar [Re: xburn]
    #4293834 - 06/14/05 01:59 AM (11 years, 7 months ago)

If you use more than a drop, you will probably fail. Too much liquid WILL result in bacterial problems.


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