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OfflineHerbanShaman
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Registered: 07/15/04
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My hybrid may be doomed... grain isolation?
    #4290506 - 06/13/05 11:31 AM (11 years, 5 months ago)

Recently I hybridized a small statured orissa strain with some some massive treasure coast.  The result was wavy capped and quite tall.  I took a few prints and attempted to make my own spore syringe.  I took a lot of precautions to avoid contamination, but to no avail.  I knocked up 6 jars.  All of these do show mycelium growth, but all have some sort of enemy hanging out. Some have lipstick mold, and others have what looks to be either bacteria, aspergillus niger, or something else black.  Is there any hope for using tweezers to fish out just one or two grains and then putting those in a new bottle to colonize?  I have often heard that with green mold the mycelium spreads everywhere and even resides inside the culture so it is difficult to isolate from.  Is this true for other forms.  I am going to attempt one jar for sure, but I don't want to waste much time and grain since I don't have much space.  Should I just toss every jar and count my losses?  :mad2: :mad2:


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OfflinePSiFr33k
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Re: My hybrid may be doomed... grain isolation? [Re: HerbanShaman]
    #4295606 - 06/14/05 06:29 PM (11 years, 5 months ago)

id say go ahead and see if you can get a grain thats farthest away from it


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While i was in the grass, i picked them..


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OfflineRogerRabbitM
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Re: My hybrid may be doomed... grain isolation? [Re: PSiFr33k]
    #4296651 - 06/14/05 10:21 PM (11 years, 5 months ago)

Exactly how did you hybridize unlike strains?
RR


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Offlinethashaman
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Re: My hybrid may be doomed... grain isolation? [Re: RogerRabbit]
    #5010369 - 12/03/05 02:59 PM (11 years, 2 days ago)

Quote:

RogerRabbit said:
Exactly how did you hybridize unlike strains?
RR




Yea, I think this is genetically impossible to do unless you have a gene laser...???


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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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