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Offlinejohnuk
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Growing truffles
    #4290197 - 06/13/05 06:24 AM (11 years, 9 months ago)

Hi,

I've been trying to find some information on growing Mexicana truffles. Someone pointed me towards MushMush and it seems I need to be growing them on a grass seed & water medium. Has anyone tried them on anything else or found anything works better? The commercial kits mention there being brown sticky goo in their's.

Also, I'm kind of wondering what would be the best way to inoculate the jars. I want to start from raw truffles since I'm going to buy some soon anyway. As they don't spore print very well, to my knowledge, I guess I'm probably going to have to develop agar plates from small pieces of the truffle cores right?

I'd prefer not to have to open my spawn jars to inoculate the medium with chunks of agar, it increases the risk of contamination and decreases colonisation rate. Once I have a mycelium on the plate could I break it up slightly, rinse it into a solution using sterile water and then inject it?

I heard someone mentioning hydrogen peroxide in another thread. Has anyone tried this on truffle mycelium? It'd be harder to get hold of in the UK, to the point of annoyance. Chemical suppliers are very reluctant to sell to the public here (Need VAT codes and three page letters about yourself).

Thanks in advance for any help!


Edited by johnuk (06/13/05 06:37 AM)


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Offlineblackout
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Re: Growing truffles [Re: johnuk]
    #4290212 - 06/13/05 06:43 AM (11 years, 9 months ago)

Search for sclerotia.
They can be grown on many grains. You can clone commercial sclerotia, I just did some last week.
I have found all the sclerotia I have had to be weak. I thought it was just my small stones, but I got a large stone with some commercial stones. 15g of fresh sclerotia, but I would only compare it to 1-1.5g dry cubensis.
H2O2 is readily available in UK chemists. You only want the 3-6% stuff, you made need licences etc for pure H2O2


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OfflineRoseM
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Re: Growing truffles [Re: johnuk]
    #4290239 - 06/13/05 07:39 AM (11 years, 9 months ago)



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Offlinejohnuk
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Re: Growing truffles [Re: Rose]
    #4290278 - 06/13/05 08:19 AM (11 years, 9 months ago)

Thanks for the help guys.

Great post Cervantes. I agree with you on the truffles.

I don't really like the way shooms look for some reason. Something about the spore dust gets to me. I used to have really bad hayfever when I was younger. Drove me nuts! I'd end up not being able to see properly because I couldn't stop itching my eyes. So perhaps it's a kind of embedded trait to stay anyway from anything producing dusts... pollen, seeds, spores... whichever. I don't really like ferns because seem to have a similar thing going on with the back of their leaves.

I was thinking of perhaps taking some pictures and getting some information together on Mexicana growing myself. Maybe you or Don could make some suggestions for experiments and we could try out some different ideas for growing.

Did you see much difference in colonisation speed between the WBS and RGS?

I was wondering if perhaps something like a WBS/RGS flour cake might be even more effective. Or perhaps if the cake was treated with something like Peptone, which apparently enhances mycelium growth.

I don't want to case mine, I think I'll be doing all the growing in containers or bags.

What happened with the spore prints? Did anyone manage to produce any?

Thanks again!


Edited by johnuk (06/13/05 08:26 AM)


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OfflineRoseM
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Re: Growing truffles [Re: johnuk]
    #4290338 - 06/13/05 09:28 AM (11 years, 9 months ago)

Many have produced prints... my friend Don however, never tried again... not yet at least.

No need to case, if you don't want spores in the air. :wink: That said, Mexicana spore production RARELY gets out of hand... unlike cubensis. Mexicana spores are worth their weight in gold.

Mexicana is S L O W... speed is not a benefit... no matter the substrate. Only by perfecting a substrain could you really increase grow speed.

If you make enough jars, you could always harvest one or two early. Don harvested a few at 1.5 months, when he cased them. This may have contamed a casing or two... but he did get some early fruits. The casings that DID work made HUGE stones, much faster than in the jar too.

I prefer Rye Grass Seed because it is CHEAP and easily found. Also, the mycelia looks pretty on RGS. WBS is not as impressive. I figure, if you're gonna look at something growing for 3 -4 months, it may as well look nice. :wink: Also, RGS is less prone to contams. There is no doubt. RGS is easier to clean off of the sclerotia too.

Don't use flower... it is not needed.

Really, there is no need to experiment too much... the easy way is the BEST way. In mycology, this rarely happens.


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Re: Growing truffles [Re: Rose]
    #4290368 - 06/13/05 10:05 AM (11 years, 9 months ago)

hey there!

so first of, what i grew were tampanensis, but as they produce stones just as
mex i thought id drop in some of my experiences aswell.

what i used as substrate was a mix of millet and rye, about 60% rye and 40% white millet.


this was loaded to jars and autoklaved, then they were inoculated by agar wedges.

i put them aside for about 4 months.


then i shook them up and harvested the sclerotia.



mmmh, gotta love sclerotia, potent lil fuggers.

anyhow, rye berries, rye grass seed and even millet, it all works quite nice
for sclerotia production and in terms of speed and bioefficiency,
the mex is even superior!

so have fun with yours,
id recommend growing them out on agar for starters still.
peace ohm :mushroom2:


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Offlinejohnuk
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Re: Growing truffles [Re: Rose]
    #4295109 - 06/14/05 04:28 PM (11 years, 9 months ago)

Quote:

Cervantes said:
If you make enough jars, you could always harvest one or two early. Don harvested a few at 1.5 months, when he cased them. This may have contamed a casing or two... but he did get some early fruits. The casings that DID work made HUGE stones, much faster than in the jar too.




Interesting!

Do you think the accelorated growth once cased could be something to do with oxygen accessibility? That's about the only thing I can think of as being majorly different between the two.


Edited by johnuk (06/14/05 04:29 PM)


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Offlinejohnuk
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Re: Growing truffles [Re: ohmatic]
    #4295122 - 06/14/05 04:31 PM (11 years, 9 months ago)

Thanks Ohm, I think I've seen some of your information elsewhere. It might have been here or do you have a site?

I think you were making up spawn bags and were soaking the seeds to saturate them with water before use.

If anyone has any links to where I can pick up things like MAE in the UK that'd be really handy!


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Offlineblackout
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Re: Growing truffles [Re: Rose]
    #4298073 - 06/15/05 04:53 AM (11 years, 9 months ago)

Quote:

Cervantes said:
The casings that DID work made HUGE stones, much faster than in the jar too.




Cervantes- I would really value your opinion on this thread.
It was in advanced for a week but got no response, I presume water was being added to your friends casing layer which I think is important.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4266601/an/0/page/1


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Edited by blackout (06/15/05 04:55 AM)


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OfflineRoseM
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Re: Growing truffles [Re: blackout]
    #4298398 - 06/15/05 10:23 AM (11 years, 9 months ago)

A little mist was sprayed, and the casing layer added moisture, and yes, they got more air. I imagine these combined things helped... the casing was NEVER dunked.


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