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Offlinedavesj1
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how long to see growth on wbs?
    #4286253 - 06/12/05 12:25 PM (11 years, 5 months ago)

how long would it take to see some growth on bags of wbs inoculated with lc at room temperature.


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InvisibleHolydiver
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Re: how long to see growth on wbs? [Re: davesj1]
    #4286260 - 06/12/05 12:28 PM (11 years, 5 months ago)

Depends, what is "room temperature"? At 75-85F you can expect a liquid culture to show signs of growth in as little as 2 days, providing WBS moisture content is O.K. and you used an adequate amount of inoculant.


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InvisibleTrav
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Re: how long to see growth on wbs? [Re: davesj1]
    #4286261 - 06/12/05 12:29 PM (11 years, 5 months ago)

Anywhere from 2 days to a week? Maybe a bit longer. That is what I would say.


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Invisibleagar
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Re: how long to see growth on wbs? [Re: davesj1]
    #4286373 - 06/12/05 12:57 PM (11 years, 5 months ago)

Quote:

davesj1 said:
how long would it take to see some growth on bags of wbs inoculated with lc  at room temperature.




Depends on quailty of the LC, the amount of WBS, how it was prepared, in what size bag, if bag had proper filter patch,the WBS preperation - sterilization method - how effective it was,  the bag sealing method, the WBS moisture content at inoculation, the amount of LC inoculate, the handling of the bag  & if the bag is incubated at optimal tempuratures? Lots of variable to ponder :confused:.

With all things optimal,I have seem very significant initial colonization being established - within 15 hours.


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InvisibleHolydiver
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Re: how long to see growth on wbs? [Re: agar]
    #4286414 - 06/12/05 01:09 PM (11 years, 5 months ago)

Quote:

agar said:
With all things optimal,I have seem very significant initial colonization being established - within 15 hours.




Maybe under a microscope?


--------------------
To find a place to live between the negatives and positives.


Edited by Diver (06/12/05 01:17 PM)


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