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OfflineMindzpore
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various liquid culture media
    #4274879 - 06/09/05 04:13 AM (18 years, 11 months ago)

I'm thinking to do some trials of different LC's. why? well, i personally find it interesting, and i think it might be of benefit with a side by side comparison of various types of media.

also, with my current situation/space this is about as ambitious a project as can be done.

my plan is to use around 45 small glass jars (250ml). using three jars for each media. this will allow me to compare 15 types of media.

they will all be nocced at the same time, with the same amount of the same monoculture from another LC. and resulting growth recorded (with pictures) on a regular basis.

Now, it will be at least a month and a half before i can really start this. but untill then i would appreciate any kind of input and suggestions. especially i would like suggestions on what type of media you think should be included (your favourites, or crazy/creative ideas). and also of course on method.

Mz


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Offlineblackout
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Re: various liquid culture media [Re: Mindzpore]
    #4274891 - 06/09/05 04:21 AM (18 years, 11 months ago)

I think they should all have the same sugar solution, calculated correctly, i.e. honey is not 100% sugar, I don't think karo is either. Identical jars, heat, filters etc.

I would like to see simple stuff readily available rather than fancy expensive or inaccessible stuff like peptone, bee pollen, vanilla or elephant dung!

Definitely coffee, apple juice. Maybe non sugar teas with added glucose/dextrose, like manure or straw tea. Or water vegetables were boiled in.

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OfflineMindzpore
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Re: various liquid culture media [Re: blackout]
    #4275054 - 06/09/05 06:59 AM (18 years, 11 months ago)

i could even do it several times, using different strains or speices. that should make clear what solutons suit what type better.


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".

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OfflineMindzpore
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Re: various liquid culture media [Re: Mindzpore]
    #4278686 - 06/10/05 02:55 AM (18 years, 11 months ago)

what? no suggestions?


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".

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Offlinecaptmueller
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Re: various liquid culture media [Re: Mindzpore]
    #4278726 - 06/10/05 03:14 AM (18 years, 11 months ago)

i have an experiment with some potato water from making agar mixed with karo water incubating now, i suppose we will see in a few days...

namaste,
captmueller

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InvisiblePinback
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Re: various liquid culture media [Re: Mindzpore]
    #4278932 - 06/10/05 06:52 AM (18 years, 11 months ago)

How do you plan on quantifying the growth?

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Offlinepsilocyben
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Re: various liquid culture media [Re: Pinback]
    #4279210 - 06/10/05 09:34 AM (18 years, 11 months ago)



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OfflineMindzpore
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Re: various liquid culture media [Re: Pinback]
    #4290191 - 06/13/05 04:17 AM (18 years, 11 months ago)

Quote:

Pinback said:
How do you plan on quantifying the growth?




well, at first i was thinking to filter, dry and weigh the stuff... but then... i just relaxed. the idea is to see if/what media gives quicker growth. and if the difference is just a few 0.x grams... its completely irrelevant. so ill just go with visual inspection. and pictures. if i cant see a difference in growth speed. it doesnt matter.

another factor that originally interested me was to see if different types of media tended to trigger different types of mycelia development. however, this will obviously be easier to see on an agar plate and will have to wait for a later experiment.


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".

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Offline_OttO_
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Re: various liquid culture media [Re: Mindzpore]
    #4540530 - 08/15/05 11:27 PM (18 years, 9 months ago)

I have a bag of dry, powdered apple tea which when mixed with hot water dissolves completely. Bought in a small village on the south coast of Turkey - the guy says he makes it himself, and unlike the 'commercially' available brands they have everywhere in Turkey that are mainly sugar, he says his homemade stuff is 90% apples. I think this is going to be promising.....

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OfflineHuckleBones
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Re: various liquid culture media [Re: _OttO_]
    #4540644 - 08/16/05 12:02 AM (18 years, 9 months ago)

I've been really meaning to try semen.


I suppose I'll eat a lot of fruit sugars and other things and hopefully my semen will have enough nutes.


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Manitou

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OfflineHotnuts
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Re: various liquid culture media [Re: Mindzpore]
    #4540700 - 08/16/05 12:20 AM (18 years, 9 months ago)

Pure vanilla extract..................

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OfflinegrimR
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Re: various liquid culture media [Re: Hotnuts]
    #5534604 - 04/19/06 06:03 PM (18 years, 1 month ago)

pure vanilla extract works? I thought that had a high alcohol content, you sure its mycellium?


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- grimR


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OfflineFeelers
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Re: various liquid culture media [Re: grimR]
    #5535699 - 04/19/06 09:56 PM (18 years, 1 month ago)

Yeah man in the vanilla extract I've seen its 50% alcohol, so I'm geussing that ones a different type???

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OfflineSnaggletooth
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Re: various liquid culture media [Re: Feelers]
    #5535722 - 04/19/06 10:00 PM (18 years, 1 month ago)

when you PC it I believe the alcohol 'burns' off....


I hear it works nicely...


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Atheist Chat

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Offlinecampinman
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Re: various liquid culture media [Re: Snaggletooth]
    #5537869 - 04/20/06 02:01 PM (18 years, 1 month ago)

i was thinking about trying out some vanilla the other day, got the same old crown karo again though.


id really like to see someone do a test with lots of different mediums, try and see what goes quickest, or strongest, or thickest, or whatever. cool idea man, i think alot of folks could learn from a nicely setup large-scale exp.


cheers

Edited by campinman (04/20/06 02:07 PM)

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Offlinealsnow469
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Re: various liquid culture media [Re: HuckleBones]
    #5575889 - 04/30/06 11:34 PM (18 years, 22 days ago)

Yours's or someone else's?

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Invisiblefastfred
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Re: various liquid culture media [Re: alsnow469]
    #5584597 - 05/03/06 06:30 AM (18 years, 20 days ago)
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Vanilla extract = pure foolishness.

Here is a cookbook of various standardized media types. Just omit the agar for liquid media. See the attached file. I would be quite interested to see the results if you have the time to test some of these wacky recipes.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

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I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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