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Offlinelepiota
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my 1st agar petri
    #4274500 - 06/09/05 03:08 AM (11 years, 5 months ago)

does this look right to you? all that is growing is white, but it doesn't look like those stamets pics! i inoculated it with a squirt from a LC syringe, so growth is not from centre to side but a little everywhere...



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OfflineIGnosticAbhorI
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Re: my 1st agar petri [Re: lepiota]
    #4274516 - 06/09/05 03:15 AM (11 years, 5 months ago)

Looks like cobweb.

Is it thin and hariy strain - Looking ?

-Gnostic


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OfflineHotnuts
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Re: my 1st agar petri [Re: IGnosticAbhorI]
    #4274564 - 06/09/05 03:37 AM (11 years, 5 months ago)

Yeah it's fine. Now if you wait for the mycelium to gain strength and start to see signs of rhizomorphic growth that looks very aggressive, you'll want to take that aggressive growth and transfer a tiny bit to another plate. You'll see what I mean. All of those germinations you've got on the petri are sub-strains. Now the dominant sub-strains will over run the week sub-strains and will eventually take over the whole dish. You'll want to choose 2 of these dominant sub-strains. Take a small sample of each of the sub-strains and transfer them to their own plate. This is strain isolation (cloning). This will give you the oppurtunity to seek out an agressive colonizer and fruiter without having to go through the fruiting cycle. Now just because a sub-strain is agressive, doesn't neccessarily mean that it is going to be a good fruiter or fruit at all in fact. This is why you'll want to do as many isolations as possible. If you start to see fruit appear on a petri, then you'll know you have a great fruiting sub-strain. Next time you inoculate a petri, put all the multispore inoculum and any other inoculum in the center carefully. Get some parafilm on those things. LOL :grin:


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OfflineHotnuts
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Re: my 1st agar petri [Re: Hotnuts]
    #4274572 - 06/09/05 03:40 AM (11 years, 5 months ago)

The ones at 5 oclock and dead center are the 2 that look like the most aggressive so far. I'd wait a few more days to see the aftermath and then go ahead and transfer those to their own plates. :cool: Good job.


Edited by hotnutz (06/09/05 03:51 AM)


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OfflineIGnosticAbhorI
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Re: my 1st agar petri [Re: Hotnuts]
    #4274741 - 06/09/05 04:54 AM (11 years, 5 months ago)

Quote:

This is why you'll want to do as many isolations as possible




I've read that you only want to do two or three isolates.

Mainly becuase after isolating so many times, you end up with a Non-Fruiter...

-Gnostic


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OfflineRogerRabbitM
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Re: my 1st agar petri [Re: IGnosticAbhorI]
    #4274752 - 06/09/05 04:58 AM (11 years, 5 months ago)

"Mainly becuase after isolating so many times, you end up with a Non-Fruiter..."

Nope. Isolate right to a single sector. This might mean you end up with twenty or so nice looking isolates. Five to ten might not fruit at all, five will fruit poor to mediocre, but five will totally kick. Those are the five you'll want to save to grow out.
RR


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OfflineHotnuts
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Re: my 1st agar petri [Re: RogerRabbit]
    #4274982 - 06/09/05 07:59 AM (11 years, 5 months ago)



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OfflineMych
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Re: my 1st agar petri [Re: Hotnuts]
    #4275307 - 06/09/05 11:53 AM (11 years, 5 months ago)

Quote:

hotnutz said:
http://mycotopia.net/discus/messages/5/41777.html?1067230771




holy fuck, i need to do this. my friend gave me a bunch of agar dishes and tissue culture boxes.. now i just need some agar and parafilm


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OfflineMych
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Re: my 1st agar petri [Re: Hotnuts]
    #4275343 - 06/09/05 12:09 PM (11 years, 5 months ago)

Quote:

hotnutz said:
http://mycotopia.net/discus/messages/5/41777.html?1067230771



ok sorry but i have to quote this again. after reading even more of that page my eyes have been opened sooooooooooooooo much THANK YOU FOR THIS LINK!!!!

i am only on my first grow (currently at the pinning stage), but this answers so many questions. More so, this answers the questions everyone has of which strain is the most potent, highest yielding, fastest growing, etc...  the answer is: none, its all about the sub strains. This is why certain cakes/casings will perform different even if they came from the same syringe with identical grow condition.. hell this is why each flush is different.

my god...  :eek: :eek: :eek: :eek: :eek: :mushroom2:


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Offlinelepiota
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Re: my 1st agar petri [Re: Mych]
    #4276344 - 06/09/05 04:23 PM (11 years, 5 months ago)

thanx for the link hotnuts, i'll try to mess a little with substrains now! the agar mix is quite an unusual one:

tap water: 250 ml
agar: 2 gr
cane sugar: 3 gr
dry dog food: 3 gr

and of course i have made more than one petri, here's the others:



i will probably isolate the most aggressive one (2nd on bottom, same of yesterday's pic) on a different recipe agar (MEA).


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OfflineHotnuts
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Re: my 1st agar petri [Re: lepiota]
    #4276361 - 06/09/05 04:29 PM (11 years, 5 months ago)

Yeah! I thought you guys would enjoy that write up. He does great work as you can see. It really helped me to understand isolations a bit more too. Good luck guys!


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Invisiblemyndreach
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Re: my 1st agar petri [Re: IGnosticAbhorI]
    #4276371 - 06/09/05 04:31 PM (11 years, 5 months ago)

Quote:

IGnosticAbhorI said:
Looks like cobweb.

Is it thin and hariy strain - Looking ?

-Gnostic




I don't think so...more like pre-rhyzomorph mycelium from my point of view.


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Offlinelepiota
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Re: my 1st agar petri [Re: myndreach]
    #4276410 - 06/09/05 04:41 PM (11 years, 5 months ago)

cobweb?
let's try to convince ourselves that cobweb is a myth.
cobweb does not exist.
even if it does, let's live as if it doesn't.
it helps, believe me, and repeat:
cobweb mold does NOT exist!


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OfflineHotnuts
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Re: my 1st agar petri [Re: lepiota]
    #4276642 - 06/09/05 05:54 PM (11 years, 5 months ago)

There's no cobweb in those dishes. Cobweb really likes very moist conditions. In which petris do not have. If it does grow in petris, it doesn't get far like trich or asperiligus will.


Edited by hotnutz (06/09/05 05:56 PM)


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