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Offlineziploker
aka..theherbsmoker
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Registered: 05/27/05
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did i screw up ?
    #4267595 - 06/07/05 02:01 PM (12 years, 6 months ago)

yesterday i pc'ed 12 jars and i left them over nite in the closed pc.. then today when i started to inoculate them i noticed when i peeled the foil off some of the jars there was water droplets on the underside of the tinfoil and some water seeped into the 4 small holes in the lid of the jar, causing some of the vermiculite to get wet, but no standing water tho..


Also, while inoculated the jars i would put a little pressure on the needle to try and inject 1/4 cc, and it would get a little stuck, then i would apply a bit more pressure and BAM.. like a whole cc would go into one hole.. lol.. so the last few jars i was only able to to put a half cc per jar... how much longer u think it would take those jars to colonize.. double the time?

all jars are now in the incubator.. i'll post some pics in a few days to see what u think..

thanks for any feedback...

is this bad?


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OfflineGNIOM1498
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Re: did i screw up ? [Re: ziploker]
    #4267617 - 06/07/05 02:05 PM (12 years, 6 months ago)

you should be ok that has happened to me before. Some did of course get contaminated so just put them in a clean area.


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
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Re: did i screw up ? [Re: ziploker]
    #4267627 - 06/07/05 02:08 PM (12 years, 6 months ago)

You should be fine. Half a cc is more than enough so long as you agitated the syringe. Also, if you use some of your syringe and decide you're going to need more you can always just suck up some sterile water and turn a few cc's back into 10. Better yet, make a LC with the honey or karo tek and turn half a cc into hundreds of ccs of liquid mycelium, which colonizes in almost half the time. Good Luck


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OfflineLengthyFellow
I play with poo

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Re: did i screw up ? [Re: LengthyFellow]
    #4267631 - 06/07/05 02:09 PM (12 years, 6 months ago)

BTW do you have any protection from contams aside from the top verm layer?


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Offlineziploker
aka..theherbsmoker
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Registered: 05/27/05
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Re: did i screw up ? [Re: LengthyFellow]
    #4267925 - 06/07/05 03:25 PM (12 years, 6 months ago)

Quote:

LengthyFellow said:
BTW do you have any protection from contams aside from the top verm layer?




well besides the clean working area, i used an alchol burner lamp to burn the tip of the needle, then i wiped it with an alchol soaked paper towel.. Then for incubation i'm using a TiT, temp reads 82 deg at the base of the tub..

think this will be ok?


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OfflineGatorade
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Registered: 05/17/05
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Re: did i screw up ? [Re: ziploker]
    #4268657 - 06/07/05 06:01 PM (12 years, 6 months ago)

Its all about learning... and that's what this experience has shown you.

I fooked up my first time with my jars, too much water...  it was moist inside and I thought I'd have 12 jars of mold.  But now they are days away from being done and I realize I shouldn't have stressed so much.

If you inoculated already, then there is nothing to do except wait it out and see.  I don't think you will be disappointed, in a few weeks you are going to have your shrooms and all will be good. :smile:


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