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Offline5meopsyco
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Registered: 02/27/05
Posts: 179
Last seen: 11 years, 11 months
antibiotic agar
    #4267320 - 06/07/05 12:39 PM (12 years, 4 months ago)

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/466150/an/0/page/0

in this link it says spores will germinate on antibiotic agar, is this true for all antibiotics, i have amp and kan not sure of the full names, will these work for germinating azure or ps.cyanofibrillosa spores?


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Offlinegrow
i ^
Registered: 04/09/05
Posts: 172
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Re: antibiotic agar [Re: 5meopsyco]
    #4267337 - 06/07/05 12:45 PM (12 years, 4 months ago)

I don't know but.. you can buy antibiotic agar at fungi perfecti


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Offline5meopsyco
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Re: antibiotic agar [Re: grow]
    #4267499 - 06/07/05 01:37 PM (12 years, 4 months ago)

or i can make my own...


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
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Re: antibiotic agar [Re: 5meopsyco]
    #4267524 - 06/07/05 01:44 PM (12 years, 4 months ago)

I was under the impression that you transferred to antibiotic agar once you have a good sample of mycelium, as spores will not germinate on antibiotic agar.

I haven't yet experimented with agar, however, so i couldn't be 100% sure.


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Offline5meopsyco
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Re: antibiotic agar [Re: LengthyFellow]
    #4267543 - 06/07/05 01:48 PM (12 years, 4 months ago)

mod's could you move this to advanced i dont htink im going to get the answers i seek here...


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
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Re: antibiotic agar [Re: 5meopsyco]
    #4267551 - 06/07/05 01:51 PM (12 years, 4 months ago)

Quote:

5meopsyco said:
mod's could you move this to advanced i dont htink im going to get the answers i seek here...




I'm sure you could find answers here. Hold on a second


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
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Re: antibiotic agar [Re: LengthyFellow]
    #4267562 - 06/07/05 01:53 PM (12 years, 4 months ago)

Looks like I found your answer in your own post...and it confirms my earlier reply:

Many people seem a bit confused as to what peroxide can and can not do. Hydrogen peroxide will kill bacteria and fungal spores and inhibit the growth of yeasts. ----Unfortunately, mushroom spores fall under the category of fungal spores, so they will not germinate on peroxidated agar because they are killed on contact.---- The good part about this is that mold spores, such as spores from the dreaded green mold, fall under this category as well and are also killed upon contact with the agar surface.


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Offlinescatmanrav
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Registered: 05/08/04
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Re: antibiotic agar [Re: 5meopsyco]
    #4267619 - 06/07/05 02:06 PM (12 years, 4 months ago)

Actually thats not the answer that he was looking for.

I can understand your wanting the topic moved to advanced after a few replies that didnt help at all (sorry guys, but if you dont know what your responding to, you should respond), but be a little more patient, its only been up an hour or so, many havnt been on to look here. There are many people who know much on the subject who come to these forums, you just have to give it a little more time. Again though, I understand the frustration when you just get replies from people who havnt used agar or "buy some".

You can germinate cubies on kanamycin but I'm unsure of the other species. They should be ok, but I dont know if you'll find someone whos done that species with that antibiotic.

AMP is antimicrobial peptides, and there are many kinds...do you know what kind yours is? Some are antifungul so probably wouldnt work. If antibacterial then it should also allow spores to germinate. As I said though, exotic species of mycelium is a bit different and can be tough to germinate on antibiotic agar...I would try both, and some with out..

Peroxide kills spores, but not all antibiotics do.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
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Re: antibiotic agar *DELETED* [Re: 5meopsyco]
    #4267645 - 06/07/05 02:14 PM (12 years, 4 months ago)

Post deleted by LengthyFellow

Reason for deletion: I'm retarded



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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
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Re: antibiotic agar [Re: scatmanrav]
    #4267655 - 06/07/05 02:16 PM (12 years, 4 months ago)

Quote:

scatmanrav said:
(sorry guys, but if you dont know what your responding to, you should respond)





...should i make another assumption that you meant "shouldn't"?


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Offlinepluteus
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Registered: 08/12/03
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Re: antibiotic agar [Re: LengthyFellow]
    #4267676 - 06/07/05 02:22 PM (12 years, 4 months ago)

I germinate the spores of all kinds of mushrooms species on antibiotic agar, including P. azurescens, P. cyanescens, and P. cyanofibrillosa. I most often use gentamycin sulfate.

Hydrogen peroxide, although it inhibits the growth of many microorganisms, is not an 'antibiotic' in the strict sense of the word.


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Offlinescatmanrav
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Registered: 05/08/04
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Re: antibiotic agar [Re: LengthyFellow]
    #4267680 - 06/07/05 02:24 PM (12 years, 4 months ago)

Yes you should :smile: Typing quick, things like that happen.

However still that part of his post wasnt really needed (which is why I missed it, skimmed over it)...the important part is the antibiotics which he has and if they'll work..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offline5meopsyco
Stranger

Registered: 02/27/05
Posts: 179
Last seen: 11 years, 11 months
Re: antibiotic agar [Re: scatmanrav]
    #4267708 - 06/07/05 02:30 PM (12 years, 4 months ago)

double posted, but hey what temps does kan break down at? again this should be archived...


Edited by 5meopsyco (06/07/05 02:33 PM)


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Offline5meopsyco
Stranger

Registered: 02/27/05
Posts: 179
Last seen: 11 years, 11 months
Re: antibiotic agar [Re: scatmanrav]
    #4267709 - 06/07/05 02:30 PM (12 years, 4 months ago)

yah in the link i posted it DID say spores will germ on antibiotics but like so many ppl posting here i was un sure, i will try the kan with a few dishes and see if they will help clean up these wild prints i have...this should be in the archives..


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 6 months
Re: antibiotic agar *DELETED* [Re: scatmanrav]
    #4267712 - 06/07/05 02:31 PM (12 years, 4 months ago)

Post deleted by LengthyFellow

Reason for deletion: Im retarded



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Edited by LengthyFellow (06/07/05 02:36 PM)


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Offline5meopsyco
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Posts: 179
Last seen: 11 years, 11 months
Re: antibiotic agar [Re: LengthyFellow]
    #4267728 - 06/07/05 02:36 PM (12 years, 4 months ago)

heres what i was refering to...

A good idea would be to order some antibiotic agar from FP to use for germinating spores. This stuff, while resistant to bacteria, is still susceptible to contamination by mold and yeast, so don't forget to use the proper sterile techniques (like a glove box, bleached down room, or a warm oven). Once the spores germinate, the mycelium can be transferred to peroxidated agar with less of a stress on sterility. It is important to remember to keep your scalpels clean and to thoroughly clean and sterilize your plates prior to pouring! If there are small mold colonies left in the plates when you pour them, they will be resistant to the peroxide and will grow.


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Offline5meopsyco
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Registered: 02/27/05
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Last seen: 11 years, 11 months
Re: antibiotic agar [Re: 5meopsyco]
    #4267738 - 06/07/05 02:38 PM (12 years, 4 months ago)

umm and just to help you out a little peroxide is not an antibiotic...


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 6 months
Re: antibiotic agar [Re: 5meopsyco]
    #4267741 - 06/07/05 02:38 PM (12 years, 4 months ago)

ya i just read that, my apologies. I thought the whole reason they sold antibiotic AND non-antibiotic is because u couldnt germinate on antibiotic, because then why would anyone even use non-antibiotic...But i think i may be associating "peroxided" and "antibiotic" too closely.


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 6 months
Re: antibiotic agar [Re: 5meopsyco]
    #4267746 - 06/07/05 02:40 PM (12 years, 4 months ago)

Quote:

5meopsyco said:
umm and just to help you out a little peroxide is not an antibiotic...




Ya ^^^ this is my misunderstanding. Thank you  :cool:


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Offline5meopsyco
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Registered: 02/27/05
Posts: 179
Last seen: 11 years, 11 months
Re: antibiotic agar [Re: LengthyFellow]
    #4267764 - 06/07/05 02:45 PM (12 years, 4 months ago)

which leads me to my next conclusion, antibiotics can be used to help clean up dirty prints a holelot better then regular agar....please archive this...


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