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Offlinenolank
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Registered: 07/06/04
Posts: 41
Last seen: 6 years, 9 months
incubation period for wbs quarts with liquid myc
    #4261480 - 06/05/05 10:47 PM (11 years, 5 months ago)

My friend inocculated 6 quart jars of wbs with karo mycelium 3 days ago and still hasnt seen any growth. He injected about half a syringe/per jar and was under the impression that with liquid mycelium growth could appear within 24 hours and total colonization could take about 11 days. He has no growth and is wondering what he did wrong or if it takes a little longer than he thought. He followed the teks exactly for soaking/simmering/pcing (incubating at 85) the grain and just wants to know what is up with the quarts. Any opinions/suggestions/anecdotes would be helpful


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 7 years, 7 months
Re: incubation period for wbs quarts with liquid myc [Re: nolank]
    #4261537 - 06/05/05 11:00 PM (11 years, 5 months ago)

Injecting half a syringe may have thrown off his water content...although i have heard of people using up to 5 ccs on a jar...I would tell him to be patient and if he doesn't see anything in another 3 days then start worrying.


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InvisibleQuiescence
Intriguer
 Arcade Champion: Hexxagon

Registered: 04/25/05
Posts: 315
Re: incubation period for wbs quarts with liquid myc [Re: nolank]
    #4261546 - 06/05/05 11:03 PM (11 years, 5 months ago)

Where did you inject? Against the jars walls? Did you shake the jars after injecting? If you shook them.. then it is possible the myc is growing inside where you can't see it. That has happened to me before.

Also, did you wait overnight for the jars to cool? Not just cool to the touch.. but at least 12hours after PC'ing? The grains can stay hot for a pretty long time.


~Quiescence~


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God raised up the fellow who was relaxed in the company of tax collectors, prostitutes and other outsiders, and went on to say that many of them would be welcomed to God?s banquet table long before those self-righteous people on display at church.


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OfflineYendor
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Registered: 11/05/03
Posts: 6
Loc: Fairbanks, AK
Last seen: 11 years, 5 months
Re: incubation period for wbs quarts with liquid myc [Re: Quiescence]
    #4262164 - 06/06/05 01:18 AM (11 years, 5 months ago)

How old was the karo mycelium? I've had problems with the stuff dying on me and not working to colinate jars. This was perhaps after a month of sitting in the fridge, and I'm not sure how fast they can die, but it is a possibility.


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Offlinenolank
Stranger
Registered: 07/06/04
Posts: 41
Last seen: 6 years, 9 months
Re: incubation period for wbs quarts with liquid myc [Re: Yendor]
    #4273751 - 06/08/05 11:29 PM (11 years, 5 months ago)

It has now been 7 days since inoculation and i can now see very faint nickel sized mycelium on the wbs. It took longer than expected but it finally happened so im happy. My pf pints were the same way. Took a week to start, but took 2 whole months until they were fully colonized and some of em still arent fully colonized. Thats why i switched to grains, thinking it would be faster. oh well


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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