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Offlinethem_26
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Registered: 05/02/01
Posts: 204
Last seen: 14 years, 26 days
mycelium growth in syringe
    #425681 - 10/14/01 11:04 PM (15 years, 1 month ago)

If a spore syringe (or a jar of solution) was accidently left in a growing environment and began growth, how long before it would be to late to innoculate with the syringe(jar of solution)? Is 3-4 days too long?
Also, to break up the clumps-for more even distribution, it'd be cool to suck the clump in and out of the syringe/jar a few times, right?
Theres no nutrients (honey or what have you) in the solution but the growth appears healthy(or so my friend says).


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OfflineDinoMyc
Ipsa scientiapotestas est
Registered: 11/14/99
Posts: 1,080
Last seen: 10 years, 5 months
Re: mycelium growth in syringe [Re: them_26]
    #426304 - 10/15/01 02:31 PM (15 years, 1 month ago)

This is not advanced.
----------------------------
If you made your syringe correctly you should not have much, if any (more often none), mycelium present in the spore syringe. How did you make your syringe?
If there are no nutrients, you could keep it cool to draw out how long it is viable for, but your best bet is use it quickly.


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Offlinethem_26
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Registered: 05/02/01
Posts: 204
Last seen: 14 years, 26 days
Re: mycelium growth in syringe [Re: DinoMyc]
    #427149 - 10/16/01 09:59 AM (15 years, 1 month ago)

PCed distilled water for aprox.30-40 minutes at 15psi.
lysoled, alcoholed; and inside glovebox scraped prints into respective jars.
flamed tips
sucked solution in and out of syringe to distribute spores
threw the cap on syringe and sealed with electrical tape.
My friend still has left over solution(not just syringes) with mycelium growing in it so I'm thinking it was the warm temps.
What improper prep-procedures are known to also cause this?


Edited by them_26 (10/16/01 10:11 AM)


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