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Amazon Shop: ½ Pint Jars, Agar, Ultrasonic Humidifier, Wild Bird Seed

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OfflineJihadISfreind
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Your oppinon on amount of sores per jar
    #4254198 - 06/04/05 12:09 AM (12 years, 25 days ago)

I know it says 1ml per jar but,what do you think would be the best ammount?


Edited by JihadISfreind (06/04/05 12:11 AM)


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InvisibleQuiescence
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Re: Your oppinon on amount of sores per jar [Re: JihadISfreind]
    #4254209 - 06/04/05 12:13 AM (12 years, 25 days ago)

Glad your doing some reading.

I would stick to the 1ml per jar. You can use more and it may go a bit faster.. or you may just throw off your water content and cause problems. Also, if you lose the jar.. you didn't lose as much solution. 1ml is more than enough to do the job.. especially on 1/2pint cakes. You can use .5 even.. it doesn't take much.

~Quiescence~


--------------------
God raised up the fellow who was relaxed in the company of tax collectors, prostitutes and other outsiders, and went on to say that many of them would be welcomed to God?s banquet table long before those self-righteous people on display at church.


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OfflineJihadISfreind
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Re: Your oppinon on amount of sores per jar [Re: Quiescence]
    #4254219 - 06/04/05 12:16 AM (12 years, 25 days ago)

kool thanks for not flaming me this time!!!!


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InvisibleQuiescence
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Re: Your oppinon on amount of sores per jar [Re: JihadISfreind]
    #4254232 - 06/04/05 12:20 AM (12 years, 25 days ago)

If your honestly trying and reading and learning yourself.. then anyone here will be happy to help you.

Good luck and have fun

~Quiescence~


--------------------
God raised up the fellow who was relaxed in the company of tax collectors, prostitutes and other outsiders, and went on to say that many of them would be welcomed to God?s banquet table long before those self-righteous people on display at church.


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Offlinescatmanrav
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Re: Your oppinon on amount of sores per jar [Re: Quiescence]
    #4254520 - 06/04/05 01:59 AM (12 years, 25 days ago)

>I know it says 1ml per jar but,what do you think would be the best ammount?

Well............1ml per jar.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisibledeanofmean
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Re: Your oppinon on amount of sores per jar [Re: scatmanrav]
    #4254704 - 06/04/05 03:01 AM (12 years, 25 days ago)

it depends on how dark your syringe is. :smile:
and, you could inject .25cc into a pint of liquid culture, and let it grow.
then suck up that white stuff into a sterile syringe
and get up to 100+ inoculations from the original syringe. :smile:


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Invisiblemyndreach
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Re: Your oppinon on amount of sores per jar [Re: deanofmean]
    #4254743 - 06/04/05 03:11 AM (12 years, 25 days ago)

AFOAF put 1cc (1ml) in some, and 2 in some and 3 in a couple.

The 3 are at 100%, the 2 are at 85%, and the 1s are at 70%.

The more spores, the faster the colonization, at least i my friend of a friend's experience.


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Invisibleagar
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Re: Your oppinon on amount of sores per jar [Re: JihadISfreind]
    #4254857 - 06/04/05 03:42 AM (12 years, 25 days ago)

Quote:

JihadISfreind said:
I know it says 1ml per jar but,what do you think would be the best ammount?




"sores".... those might be tough to get in a syringe (joke).

Size & content of jar make a differance. My foaf uses 2.5 ml of spores or LC into quart jars of wbs.

Filter/patch spawn bags with 5 quarts of wbs - he uses a full 10ml.

Great tool for inoculating lots of jars or bags - at one sitting.


--------------------


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OfflineBlue Helix
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Re: Your oppinon on amount of sores per jar [Re: agar]
    #4255722 - 06/04/05 01:55 PM (12 years, 25 days ago)

I routinely inoculate agar plates with a single drip of spore solution and, recently, liquid culture. Both have worked great. It all depends on how much time you have on your hands. Any amount will work in two weeks, even a single drop.


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OfflineHotnuts
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Re: Your oppinon on amount of sores per jar [Re: Blue Helix]
    #4255930 - 06/04/05 02:47 PM (12 years, 25 days ago)

I've got a foaf that uses up to 5 cc's for 1/2 pints. No probs at all.


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Offlineglace
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Re: Your oppinon on amount of sores per jar [Re: Blue Helix]
    #4255966 - 06/04/05 03:01 PM (12 years, 25 days ago)

I recently had to water down a syringe because my cousin stole my cakes (and allegedly ate them!) As I bought the syringe and didn't have a print of the variety I added some water to the syringe. I then used it to inoculate some more cakes. Each ml in the syringe was worth 0.1ml of the liquid originally in the syringe but the cakes still colonized in <2 weeks. The time taken from when I inoculated the jars to the time I picked the first flush was only three days longer than that for my previous grow.


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InvisibleQuiescence
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Re: Your oppinon on amount of sores per jar [Re: agar]
    #4255985 - 06/04/05 03:10 PM (12 years, 25 days ago)

Quote:

agar said:

Great tool for inoculating lots of jars or bags - at one sitting.




Oh my!
You could inoculate a small ocean with that thing. Goodness...


--------------------
God raised up the fellow who was relaxed in the company of tax collectors, prostitutes and other outsiders, and went on to say that many of them would be welcomed to God?s banquet table long before those self-righteous people on display at church.


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OfflineIGnosticAbhorI
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Re: Your oppinon on amount of sores per jar [Re: Quiescence]
    #4256013 - 06/04/05 03:23 PM (12 years, 25 days ago)

Damn, thats a waste of solution HN lol.

Might even mess with the water content....

5cc's per 1/2 pint?

Damn, I use about 1cc per pint and 1.5-2ccs's per quart...

BH ive heard uses barely Anything for Agar plates lol

-Gnostic


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OfflineCLUTCH
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Re: Your oppinon on amount of sores per jar [Re: deanofmean]
    #4258206 - 06/05/05 02:14 AM (12 years, 24 days ago)

Quote:

deanofmean said:
it depends on how dark your syringe is. :smile:





I think there are many that would disagree with this.

I know many vendors do.  Correct me if I'm wrong, but even clear syringes can have as many spores as the dark ones.


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OfflineHotnuts
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Re: Your oppinon on amount of sores per jar [Re: IGnosticAbhorI]
    #4258232 - 06/05/05 02:22 AM (12 years, 24 days ago)

Not if you're trying to get shit rollin. LOL! I think 12-15 days for colonization is better than 30 any day. If you're worried with wasting spore solution, you need to look into liquid cultures. I use 6 cc's of multispore per 1qt jar, and then use that 1qt jar for G2G to several dozen more. :wink:


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Offlinehawksapprentice
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Re: Your oppinon on amount of sores per jar [Re: CLUTCH]
    #4258242 - 06/05/05 02:25 AM (12 years, 24 days ago)

No, they will have ALOT more. The arguement is that even if the syringe is clear it can still have thounsands to millions of spores in it. If the syringe is dark then it obviously has more spores. But the real debate is wether a darker syringe will work better.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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Offlinehawksapprentice
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Re: Your oppinon on amount of sores per jar [Re: hawksapprentice]
    #4258246 - 06/05/05 02:26 AM (12 years, 24 days ago)

And Agar.  What type of gun is that, how is it powered and where can I get one :grin:


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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OfflineHotnuts
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Re: Your oppinon on amount of sores per jar [Re: hawksapprentice]
    #4258259 - 06/05/05 02:31 AM (12 years, 24 days ago)

Quote:

hawksapprentice said:
No, they will have ALOT more. The arguement is that even if the syringe is clear it can still have thounsands to millions of spores in it. If the syringe is dark then it obviously has more spores. But the real debate is wether a darker syringe will work better.




If it's sonicated it will definately work better. The more spores the marrier IMO. Gives you way more sub-strains to work with.


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Offlinehawksapprentice
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Re: Your oppinon on amount of sores per jar [Re: Hotnuts]
    #4258272 - 06/05/05 02:34 AM (12 years, 24 days ago)

What do you use to sonicate your spores? And yes i prefer a dark syringe. But i have seen excellent results from both.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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OfflineHotnuts
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Re: Your oppinon on amount of sores per jar [Re: hawksapprentice]
    #4258281 - 06/05/05 02:37 AM (12 years, 24 days ago)

I've had great results with clearer syringes also. I bought an Ultrasonic jewelry cleaner. It works a hell of a lot better than my humidifier. I would set them on top of my humidifier and they would disperse some, but nothing like the jewelry cleaner.


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Amazon Shop: ½ Pint Jars, Agar, Ultrasonic Humidifier, Wild Bird Seed

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