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Ok..... I have sucrose, gelatin and tryptose phosphate broth, all of which are pure laboratory grade reagents. I have little experience with fungi. I thought of maybe using the gelatin in place of agar and adding dextrose and sucrose. Any ideas? I was also wondering if the tryptose phosphate broth would work at all.
Tryptose phosphate broth is used in insect cell cultures. It was a long shot. What about gel agarose? I figure that it's just agar w/ some sort of sugar in it, but then again it could just be a Sigma brand name or something. Are there any disadvantages to using gelatin as oppose to agarose? Thank you puscle for your reply. I appreciate it. I don't understand why people get all bent out of shape. If you think I'm full of whatever don't reply. One more thing. Does anyone know if there are any strong odors that result when you autoclave vermiculite/brown rice flour substrate?
I'm figuring that if you have access to an autoclave, you can probably find some agar. I wouldn't use agarose cuz I don't know how well it works with other stuff (like dextrose) dissolved in it. I find a distinct grainy smell when the autoclave door unseals at the end of the cycle. It doesn't smell like anything else we usually autoclave. Hint, wait til every one else goes home and then say you are working late. That's what I do cuz I don't want anyone seeing me putting jars of dirt into the autoclaves.
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