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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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North Spore Shop: Bulk Substrate, Cultivation Supplies

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Invisiblecheesenoonions
??????????????

Registered: 04/01/01
Posts: 584
Making Media
    #425298 - 10/14/01 03:13 PM (22 years, 1 month ago)

Ok..... I have sucrose, gelatin and tryptose phosphate broth, all of which are pure laboratory grade reagents. I have little experience with fungi. I thought of maybe using the gelatin in place of agar and adding dextrose and sucrose. Any ideas? I was also wondering if the tryptose phosphate broth would work at all.


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Offlineshroomme
journeyman
Registered: 10/04/01
Posts: 51
Loc: North East
Last seen: 22 years, 14 days
Re: Making Media [Re: cheesenoonions]
    #425361 - 10/14/01 04:09 PM (22 years, 1 month ago)

ummmmmmmmmmmmmmm ha?
lol


--------------------
" When I woke and found you missing,,,,,,,, I looked but,,,,,, I could not see youuuuuu...."


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Invisiblebigwill
member
Registered: 11/29/99
Posts: 207
Re: Making Media [Re: cheesenoonions]
    #426236 - 10/15/01 10:37 AM (22 years, 1 month ago)

yeah sure bud.


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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: Making Media [Re: cheesenoonions]
    #426294 - 10/15/01 12:20 PM (22 years, 1 month ago)

Mix your gelatin per instructions, add 5% sucrose, then pressure cook. What is tryptose phosphate?
Now what the fuck is up with you newbies making fun of this guy`s post?


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Invisiblecheesenoonions
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Registered: 04/01/01
Posts: 584
Re: Making Media [Re: cheesenoonions]
    #426319 - 10/15/01 12:46 PM (22 years, 1 month ago)

Tryptose phosphate broth is used in insect cell cultures. It was a long shot. What about gel agarose? I figure that it's just agar w/ some sort of sugar in it, but then again it could just be a Sigma brand name or something. Are there any disadvantages to using gelatin as oppose to agarose? Thank you puscle for your reply. I appreciate it. I don't understand why people get all bent out of shape. If you think I'm full of whatever don't reply. One more thing. Does anyone know if there are any strong odors that result when you autoclave vermiculite/brown rice flour substrate?


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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: Making Media [Re: cheesenoonions]
    #426350 - 10/15/01 01:08 PM (22 years, 1 month ago)

If you don't know what gel agarose is, don't use it. You would do well to stick with a time proven Tek. Hey, I think we're all full of shit, so there. No, autoclaving substrate doesn't stink.


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Invisiblecheesenoonions
??????????????

Registered: 04/01/01
Posts: 584
Re: Making Media [Re: cheesenoonions]
    #426362 - 10/15/01 01:20 PM (22 years, 1 month ago)

I'm sorry I meant to ask if there were any dissadvantages to using gelatin as oppose to agar..... do you know of any?


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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: Making Media [Re: cheesenoonions]
    #426363 - 10/15/01 01:25 PM (22 years, 1 month ago)

No, they are both good mediums.


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Offlinesylo
addict
Registered: 10/29/00
Posts: 219
Last seen: 21 years, 7 months
Re: Making Media [Re: puscle]
    #427075 - 10/16/01 04:17 AM (22 years, 1 month ago)

Gelatin is a good medium? Have you ever tried it? Many contams liquify gelatin and it melts at room temp.


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Offlineheathadelic
member

Registered: 07/24/01
Posts: 159
Last seen: 21 years, 9 months
Re: Making Media [Re: cheesenoonions]
    #427400 - 10/16/01 01:45 PM (22 years, 1 month ago)

I'm figuring that if you have access to an autoclave, you can probably find some agar. I wouldn't use agarose cuz I don't know how well it works with other stuff (like dextrose) dissolved in it.
I find a distinct grainy smell when the autoclave door unseals at the end of the cycle. It doesn't smell like anything else we usually autoclave. Hint, wait til every one else goes home and then say you are working late. That's what I do cuz I don't want anyone seeing me putting jars of dirt into the autoclaves.


--------------------
"...time is not money, time is an opportunity to live before you die."


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Offlinesylo
addict
Registered: 10/29/00
Posts: 219
Last seen: 21 years, 7 months
Re: Making Media [Re: heathadelic]
    #428188 - 10/17/01 03:07 AM (22 years, 1 month ago)

Be careful! I have a friend who got fired for working late. He kept the jars in his locker.


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North Spore Shop: Bulk Substrate, Cultivation Supplies

Mushrooms, Mycology and Psychedelics > Advanced Mycology
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