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Offlineblackout
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Do some endospores need to be heated a lot before germinating in grains
    #4251366 - 06/03/05 06:42 AM (11 years, 7 months ago)

Do some endospores need to be heated a lot before germinating in grains? I have a small amount of wheat grain which has been soaking for over a week now. I have rinsed it now with boiling water. It smelt a bit but more like it was fermenting, grain looks fine, only a few had germinated. I have been trying fractional sterilization and have 2 large bags colonising nicely at the moment which were F sterilized.
I am wondering if all the endospores should have germinated in my grain by now and if there is a chance that steaming would now sterilize? Rinsing the grain once a day should clean it of anything that has just started to grow on the surface. I always wondered about the 3 days in a row at 100C for 30mins incubating in between, sounds too exact and possibly overkill, I wonder if you could incubate for 3 days, boil, incubate for 3 days and boil again. There doesnt seem to be much on google about trying different heat/incubate combinations.
Would adding bleach to the soaking water help? The bleach should be destoyed upon later heating.


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Edited by blackout (06/03/05 07:40 AM)


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