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Amazon Shop for: ½ Pint Jars, Agar

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OfflineChastity_Belt
Unholy livingdead

Registered: 06/03/05
Posts: 188
Last seen: 11 years, 5 months
Agar: You're the f'in best!
    #4251310 - 06/03/05 05:10 AM (11 years, 6 months ago)

    Great LC tek!  Wonderful shit.  Thanks for sharing. 
    A question: has anyone tried to clone a bit of fruitbody tissue (taken from the inside of the stem) and had that piece of tissue grow out...as in, not growing like liquid mycelium, but an odd monstrosity branching out off of the piece of tissue used for cloning? 
   

    :laugh:


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InvisibleRoadkill
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: Agar: You're the f'in best! [Re: Chastity_Belt]
    #4251594 - 06/03/05 10:19 AM (11 years, 6 months ago)

Quote:

A question: has anyone tried to clone a bit of fruitbody tissue (taken from the inside of the stem) and had that piece of tissue grow out...as in, not growing like liquid mycelium, but an odd monstrosity branching out off of the piece of tissue used for cloning?





not sure what you mean bro...

here is a clone...on agar.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineChastity_Belt
Unholy livingdead

Registered: 06/03/05
Posts: 188
Last seen: 11 years, 5 months
Re: Agar: You're the f'in best! [Re: Roadkill]
    #4253333 - 06/03/05 08:25 PM (11 years, 6 months ago)

    Sorry--to be clearer.  I was just very happy with the malt/dextrose mix for liquid cultures.  I used a piece of a fruitbody and peroxide dipped it and dropped it into the liquid culture mix.  This would then be a clone culture of the fruitbody/mycelium from that casing, correct? 
    And in one of the jars it looks like the stem tissue is growing very strangely--not like a cloud of myc. 
    Anyway, I was just excited about Agar's liquid culture recipe as it's working far better than any karo tek I've tried.  :cool:


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OfflineHotnuts
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Registered: 02/26/05
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Re: Agar: You're the f'in best! [Re: Chastity_Belt]
    #4256023 - 06/04/05 03:27 PM (11 years, 6 months ago)

Quote:

Chastity_Belt said:
Sorry--to be clearer. I was just very happy with the malt/dextrose mix for liquid cultures. I used a piece of a fruitbody and peroxide dipped it and dropped it into the liquid culture mix. This would then be a clone culture of the fruitbody/mycelium from that casing, correct?
And in one of the jars it looks like the stem tissue is growing very strangely--not like a cloud of myc. ]



Yes. You've isolated the sub-strain. (single fruit body) The tissue will grow mycelium from it in a weird lookin way.


Edited by hotnutz (06/04/05 03:28 PM)


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InvisibleRoadkill
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Re: Agar: You're the f#ckin best! [Re: Chastity_Belt]
    #4256448 - 06/04/05 05:42 PM (11 years, 6 months ago)

Quote:

Chastity_Belt said:

I used a piece of a fruitbody and peroxide dipped it and dropped it into the liquid culture mix. This would then be a clone culture of the fruitbody/mycelium from that casing, correct?





yes that would work.

I have also taken a peice of the fruitbody and put it in a blender with sterile water and made mycelium syringes from just that.

good luck!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Amazon Shop for: ½ Pint Jars, Agar

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