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HidingInPlainSight

Registered: 01/27/03
Posts: 2,077
Loc: Oklahoma City , OK, USA
Last seen: 1 year, 8 months
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Myc culture in self healing bottle for long term storage *PiC!*
#4247461 - 06/02/05 10:02 AM (18 years, 8 months ago) |
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psilocyben
bad guy

Registered: 12/03/04
Posts: 1,026
Last seen: 17 years, 1 month
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: HidingInPlainSight]
#4247496 - 06/02/05 10:16 AM (18 years, 8 months ago) |
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pretty neat- where'd you find the bottle?
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myndreach
philosopher



Registered: 08/07/04
Posts: 2,368
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: psilocyben]
#4247509 - 06/02/05 10:21 AM (18 years, 8 months ago) |
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ncie bottle...where did you get it?
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HidingInPlainSight

Registered: 01/27/03
Posts: 2,077
Loc: Oklahoma City , OK, USA
Last seen: 1 year, 8 months
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: HidingInPlainSight]
#4247530 - 06/02/05 10:29 AM (18 years, 8 months ago) |
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I actually have to take allergy shots.  My medicine comes in those bottles. So I injected the bottle with 70/30 iso/water and shook it up, then flushed the bottle twice with boiling water. After that it was filled half way with sterile water and then an LC syringe was used to transfer the myc into the bottle.
Edited by HidingInPlainSight (06/02/05 10:30 AM)
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Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 month, 19 days
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: HidingInPlainSight]
#4247550 - 06/02/05 10:39 AM (18 years, 8 months ago) |
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Is the bottles injection site self healing? Neat!
Edited by hotnutz (06/02/05 10:44 AM)
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 30 days
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: Hotnuts]
#4247556 - 06/02/05 10:40 AM (18 years, 8 months ago) |
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Nice thinking, never thought of that lol.
5 for you 
Mini cults AHhh hAhhaH
-Gnostic
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HidingInPlainSight

Registered: 01/27/03
Posts: 2,077
Loc: Oklahoma City , OK, USA
Last seen: 1 year, 8 months
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: Hotnuts]
#4247643 - 06/02/05 11:15 AM (18 years, 8 months ago) |
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Quote:
hotnutz said: Is the bottles injection site self healing? Neat!
Yes it is.
What I plan on doing is taking the 1cc allergy syringes, and pulling a little of that liquid out and making a whole new LC.. while still retaining 90% of the mycelium left in the self healing bottle!
Also keep in mind those bottles only hold around 2-3cc's!
Edited by HidingInPlainSight (06/02/05 11:16 AM)
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Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 month, 19 days
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: HidingInPlainSight]
#4247649 - 06/02/05 11:17 AM (18 years, 8 months ago) |
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That would be a neat way to store a master culture in the fridge. I wonder how long it would survive? I'd bet it wouldn't matter. Now'a days they've proven that dry or dead mushrooms and mycelium can be grown out on agar plates.
Edited by hotnutz (06/02/05 11:21 AM)
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HidingInPlainSight

Registered: 01/27/03
Posts: 2,077
Loc: Oklahoma City , OK, USA
Last seen: 1 year, 8 months
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: HidingInPlainSight]
#4248516 - 06/02/05 02:37 PM (18 years, 8 months ago) |
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That's how small it is.
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waixingren


Registered: 03/14/05
Posts: 2,644
Loc: SW Florida
Last seen: 1 month, 3 days
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: HidingInPlainSight]
#4248525 - 06/02/05 02:39 PM (18 years, 8 months ago) |
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i wonder if theres a place online where those could be ordered
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deathbychimney
45th GenerationRoman
Registered: 02/26/05
Posts: 239
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: waixingren]
#4248600 - 06/02/05 02:57 PM (18 years, 8 months ago) |
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i just looked and couldn't find any
i looked through this site a little bit but there is a shitload of glassware they have for sale, it seems like it might be there somewhere though.
www.labx.com at theleast if someone can just find the name of these vials it would be helpful
-------------------- diarrhea takes over your life
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 30 days
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: deathbychimney]
#4248695 - 06/02/05 03:12 PM (18 years, 8 months ago) |
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They should last for decades.
Myc. and distilled water.
-Gnostic
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HidingInPlainSight

Registered: 01/27/03
Posts: 2,077
Loc: Oklahoma City , OK, USA
Last seen: 1 year, 8 months
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Re: Myc culture in self healing bottle for long term storage *PiC!* [Re: deathbychimney]
#4248700 - 06/02/05 03:14 PM (18 years, 8 months ago) |
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I get the vials from my allergy clinic.
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