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Offlinebreeed
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Registered: 06/01/05
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Last seen: 11 years, 6 months
Is there a way to...
    #4245166 - 06/01/05 07:18 PM (11 years, 8 months ago)

curious if anyone has any information on a means of colonizing a substrate w/ just a mushroom..? or make a mixture that i could make a syringe from...i do not have a syringe or any prints at the moment...i do have a couple grams of somewhat fresh mushrooms. (p. cubensis variety.) any help would be appreciated. there was a honey tek that i found very interesting.

-j.r.s.


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OfflineCray
I'm not fat i'mbig bones
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Registered: 02/22/05
Posts: 951
Last seen: 7 years, 11 months
Re: Is there a way to... [Re: breeed]
    #4245225 - 06/01/05 07:33 PM (11 years, 8 months ago)

try getting spore prints from your fresh mushies, if you go the top look at: Mushroom Info. > Growing Mushrooms > Spore Prints and Syringes.

-Cray


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Offlineviper100fm
mustash invation

Registered: 04/07/05
Posts: 85
Loc: budtown B.C
Last seen: 7 years, 3 months
Re: Is there a way to... [Re: Cray]
    #4245263 - 06/01/05 07:40 PM (11 years, 8 months ago)

spore print is the way to go for that but im not sure if it will work with semi dried mush.. i picked some waited a day and tryed to make a print with no succses but that doesnt mean you should try :wink:


--------------------
"Whoever appeals to the law against his fellow man
Is either a fool or a coward.
Whoever can not take care of himself without that law is both.
For a wounded man shall say to his assailent
If i live i will kill you,
If i die you are forgiven,
Such is the rule of honor..."


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Invisibletahoe
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Registered: 11/27/03
Posts: 6,215
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Re: Is there a way to... [Re: viper100fm]
    #4245391 - 06/01/05 08:16 PM (11 years, 8 months ago)

if the mushroom was gown in a 100% sterile environment, then you could sterilize some water in a blender and puree that fucker until you have just blue water and mush, Then suck that up into a syringe and inoc some cakes. it works great, that is, if you are 100% sterile. So what I'm saying is, you are guaranteed to fail. :frown:


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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I need to proofread


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Offlinegrow
i ^
Registered: 04/09/05
Posts: 172
Last seen: 10 years, 7 months
Re: Is there a way to... [Re: breeed]
    #4245470 - 06/01/05 08:38 PM (11 years, 8 months ago)

if you have fresh mushrooms... take a spore print.


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OfflineKaptKid
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Re: Is there a way to... [Re: breeed]
    #4245724 - 06/01/05 09:43 PM (11 years, 8 months ago)

Try cloning. :tongue2:


:sun:


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Is there a way to... [Re: breeed]
    #4245810 - 06/01/05 10:17 PM (11 years, 8 months ago)

if no spores drop, there may still be a way.
this would probably make more sense to an experienced cultivator.that said,here we go .
first, save that mushroom cape in the cleanest place possible.
2.start reading everything you can find on agar, and liquid culture.
when you feel confident enough to try one of these, go to step 3.
3.are you sure you're ready? you have all the equipment ready, if not go back to step 2.
now if you are ready, you need several small pieces of hard cardboard. not the corrugated stuff, gray cardboard like from the back of a notepad.
sterilize this, let cool and take it and your mushroom to your glove box.
if you don't have a glove box, go back to step 2.
if you are in fact ready, take a piece of the cardboard, and dab it to the mushroom gills.
4.incubate cardboard 1 or 2 maybe 3 days.the area where the spores were will have turned white, it will look like powdered sugar.
step 5. place cardboard on peroxided agar or into liquid medium w/ 1% peroxide.
thats it, either we are done, or back to step 2.

edit* because i forgot a step :blush:


Edited by deanofmean (06/01/05 10:32 PM)


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