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Offlinelepiota
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suitable sugars for LC (experiment with pic)
    #4243250 - 06/01/05 08:55 AM (11 years, 6 months ago)

we're at day 6 of my little experiment about the best sugars we can use in liquid cultures. i have tried beet sugar (=common white table sugar), cane sugar (brownish), fructose and honey. all in a 3% solution, all in 200ml water, all inoculated with 1ml spore solution from the same siringe.
this is a pic taken at day 6:



at the moment the experiment is demonstrating what we already knew: honey is the fastest colonizing sugar, but cane sugar is also performing well.
on the other hand, i'm having poor results with both beet sugar and fructose.
so, if you don't have honey go for cane sugar!


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Edited by lepiota (06/01/05 09:06 AM)


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OfflineHippieChick
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Re: suitable sugars for LC (experiment with pic) [Re: lepiota]
    #4243282 - 06/01/05 09:30 AM (11 years, 6 months ago)

Where is dextrose?

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OfflineShdwstr
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Re: suitable sugars for LC (experiment with pic) [Re: HippieChick]
    #4243288 - 06/01/05 09:35 AM (11 years, 6 months ago)

... and Karo,
... and Malt extract,
... and  :smirk:


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OfflineHippieChick
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Re: suitable sugars for LC (experiment with pic) [Re: Shdwstr]
    #4243303 - 06/01/05 09:45 AM (11 years, 6 months ago)

Kind of like saying Coca-Cola is the best without even trying Pepsi, Sprite, 7-Up, Sunkist, so on and so on.

Peace,Love,Happiness and Harmony.
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--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Offlinelepiota
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Re: suitable sugars for LC (experiment with pic) [Re: HippieChick]
    #4243335 - 06/01/05 10:06 AM (11 years, 6 months ago)

karo is an american thing, it is not in shops in europe!
i can't find malt extract anywhere and dextrose is too expensive (they only sell it for body builders in big packs)
this is an experiment to find the best sugar among those i can find in my house!


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Offlinelepiota
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Re: suitable sugars for LC (experiment with pic) [Re: lepiota]
    #4243337 - 06/01/05 10:07 AM (11 years, 6 months ago)

... and pepsi sucks :smile:


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Onlineblackout
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Re: suitable sugars for LC (experiment with pic) [Re: lepiota]
    #4243404 - 06/01/05 10:41 AM (11 years, 6 months ago)

Good experiment. I planned on trying normal sugar (sucrose) myself. I have grown with straight glucose too which showed very little growth, but it did work. Malt extract is expensive for me too, available in health food shops or brewing suppliers. It is nice to be able to use something straight out of the press. Honey works well for me.
Brown or unrefined sucrose will have molasses in it which has been mentioned in published texts about making liquid cultures for mushroom flavouring, so I thought it would work well.
Small packs of brown sugar can be picked up and sometimes have the weight on them which is useful for people without scales or with inaccurate scales at the gram level.

I think apple juice may work too.


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Offlinelepiota
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Re: suitable sugars for LC (experiment with pic) [Re: blackout]
    #4243469 - 06/01/05 11:05 AM (11 years, 6 months ago)

by the way, what's the best brewing malt extract used for LC and agar? liquid or powdered? light, amber or dark?


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OfflineHotnuts
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Re: suitable sugars for LC (experiment with pic) [Re: lepiota]
    #4243497 - 06/01/05 11:13 AM (11 years, 6 months ago)

Quote:

lepiota said:
karo is an american thing, it is not in shops in europe!
i can't find malt extract anywhere and dextrose is too expensive (they only sell it for body builders in big packs)
this is an experiment to find the best sugar among those i can find in my house!




A damn good experiment if I say so. Thanx for sharing. :thumbup:


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Invisibleagar
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Re: suitable sugars for LC (experiment with pic) [Re: lepiota]
    #4243536 - 06/01/05 11:24 AM (11 years, 6 months ago)

Quote:

lepiota said:
by the way, what's the best brewing malt extract used for LC and agar? liquid or powdered? light, amber or dark?




LIGHT DRY MALT.....has done wonders fot me.


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Offlinelepiota
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Re: suitable sugars for LC (experiment with pic) [Re: agar]
    #4243554 - 06/01/05 11:31 AM (11 years, 6 months ago)

i have found a shop near my house who has it. maybe i'll make a larger experiment using also dextrose, malt extract, pure sucrose and... pepsi! :smile:
then, i'll also try varius mixture, any hints?


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OfflineIGnosticAbhorI
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Re: suitable sugars for LC (experiment with pic) [Re: lepiota]
    #4243568 - 06/01/05 11:38 AM (11 years, 6 months ago)

Send me a Pm,lets see if we can get you a little bit of dextrose :grin:

-Gnostic


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OfflineMindzpore
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Re: suitable sugars for LC (experiment with pic) [Re: IGnosticAbhorI]
    #4243615 - 06/01/05 11:55 AM (11 years, 6 months ago)

same here, no karo. but something called muscovado sugar (looks brown). and sugar made from sugarbeets. maby you want to try those.

also maby try the water that you have boiled pasta in, and why not a a light coffe solution.


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Offlinelepiota
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Re: suitable sugars for LC (experiment with pic) [Re: Mindzpore]
    #4243622 - 06/01/05 11:58 AM (11 years, 6 months ago)

the list grows larger and larger... i'll make 12 jars next time!


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Onlineblackout
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Re: suitable sugars for LC (experiment with pic) [Re: Mindzpore]
    #4243627 - 06/01/05 12:01 PM (11 years, 6 months ago)

Quote:

Mindzpore said:
why not a a light coffe solution.



Good idea, I would put a small bit of sucrose (table sugar) in it. I think the only problem with sucrose is the lack of nutrients, most people will have instant coffee and sugar.


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Invisibleagar
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Re: suitable sugars for LC (experiment with pic) [Re: lepiota]
    #4243661 - 06/01/05 12:15 PM (11 years, 6 months ago)

Denatured beer & apple juice will grow a healthy culture.


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OfflineSammy
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Re: suitable sugars for LC (experiment with pic) [Re: agar]
    #4243664 - 06/01/05 12:17 PM (11 years, 6 months ago)

you guys will think of anything!!!

sam


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OfflineShdwstr
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Re: suitable sugars for LC (experiment with pic) [Re: IGnosticAbhorI]
    #4243670 - 06/01/05 12:20 PM (11 years, 6 months ago)

Quote:

IGnosticAbhorI said:
Send me a Pm,lets see if we can get you a little bit of dextrose :grin:

-Gnostic




Now that's a twist... shipping a bag of white powder to Europe...
That should raise some eyebrows  :lol:


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Offlinelepiota
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Re: suitable sugars for LC (experiment with pic) [Re: Shdwstr]
    #4243675 - 06/01/05 12:22 PM (11 years, 6 months ago)

what do you mean by 'denatured' beer, agar?


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OfflineIGnosticAbhorI
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Re: suitable sugars for LC (experiment with pic) [Re: Shdwstr]
    #4243677 - 06/01/05 12:23 PM (11 years, 6 months ago)

Quote:

Shdwstr said:
Quote:

IGnosticAbhorI said:
Send me a Pm,lets see if we can get you a little bit of dextrose :grin:

-Gnostic




Now that's a twist... shipping a bag of white powder to Europe...
That should raise some eyebrows  :lol:




:shrug:

Yeah...lol...oh well...

Hey Agar, didnt you do something w/ beer and apple juice last time?

I remeber reading something like that and laughed and thought about Drunk Mushrooms...lol

-Gnostic


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Scales

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