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OfflineSmallworlds
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Registered: 03/12/05
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I have a good damn idea!!
    #4233357 - 05/29/05 02:46 PM (11 years, 10 months ago)

Tell me if you think this is good please:

There are these "oven roaster" bags that are sold in the grocery store and will survive temps up to 400F.

Instead of using jars and a pressure cooker, use these bags and an oven, monitoring the temperature with an oven thermometer.

Substrate consisting of 50% straw 50% wild bird seed is soaked overnight.

The excess moisture is squeezed out and the bags are filled with moist substrate, then the bags are tied shut.

The bags of substrate are heated to 250F in an oven for an hour to allow the center of the substrate to reach 250F sterilizing temps for at least 20 minutes.

The bags are cooled, and then inoculated using a sterile technique using sporeprints and a sterile scalpel to scrape spores into the bags.

The bags are then re-tied loosely to allow for some degree of gas exchange, and set in an approximate 80F degree environment to colonize.

________________________________________

Yes? No? Good? Bad? Did I forget something? Do you think this will this work O.K.? Thanks in advance for your comments. :thumbup:


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Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
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Edited by Smallworlds (05/30/05 01:46 AM)


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OfflineHengman
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4233480 - 05/29/05 03:18 PM (11 years, 10 months ago)

seems legit to me. how did u get the sporeprints in the bag?

you may have a contam problem because there is nothing protecting the contents of the bag during the gas exchange


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OfflineHippieChick
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4233495 - 05/29/05 03:20 PM (11 years, 10 months ago)

You might want to rehydrate those spores in a syringe for a little bit before using to inoculate.

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Offlinepsilocyben
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4233500 - 05/29/05 03:22 PM (11 years, 10 months ago)

Quote:

Smallworlds said:
Do you think this will this work O.K.?




i'm pretty confident that that procedure would result in failure.

it sounds like you are looking for a shortcut, no?

if you don't own a pressure cooker, buy one; in the mean time, you can always do brf cakes and spawn them to pasturized horse poo.

probably the best way to get somethin for nothin.
:zoom:


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OfflineMiscusi
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Re: O.K. a couple of questions please... [Re: HippieChick]
    #4233501 - 05/29/05 03:23 PM (11 years, 10 months ago)

Quote:

HippieChick said:
You might want to rehydrate those spores in a syringe for a little bit before using to inoculate.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:




True, unless its a fresh print, then you'll be ok


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OfflineGodspeed
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Re: O.K. a couple of questions please... [Re: Miscusi]
    #4233541 - 05/29/05 03:40 PM (11 years, 10 months ago)

the substrate will dry out quite a bit in the oven i'd imagine


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OfflineSmallworlds
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Registered: 03/12/05
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Re: O.K. a couple of questions please... [Re: Godspeed]
    #4234042 - 05/29/05 07:18 PM (11 years, 10 months ago)

thx. it's fresh prints and the bags hold in the moisture so they don't dry out while in the oven.

my biggest concerns are actually excess moisture and contams.

time will tell, i'll let you know what happens


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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OfflineSmallworlds
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Re: O.K. a couple of questions please... [Re: psilocyben]
    #4234066 - 05/29/05 07:28 PM (11 years, 10 months ago)

Quote:

psilocyben said:
Quote:

Smallworlds said:
Do you think this will this work O.K.?




i'm pretty confident that that procedure would result in failure.

:zoom:




I'm curious what exactly about the proceedure outlined do you think wouldn't work?

This is just an experiment, I'm not claiming it's gonna work, but I'd appreciate any help in fine-tuning this particular line of experiments with the oven bags so this might work better. any ideas are appreciated, particularly with sterilization temps and times as they apply to this experiment. and moisture content of substrate. etc. etc.


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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OfflineGNIOM1498
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4235093 - 05/30/05 01:53 AM (11 years, 10 months ago)

if u want to get it up to 250F in the oven make sure there is no moisture in it because it would be impossible to get up to 250 with moisture in ur bags.


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Offlinepsilocyben
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Re: O.K. a couple of questions please... [Re: psilocyben]
    #4235117 - 05/30/05 02:02 AM (11 years, 10 months ago)

it's not so much how you're doing it, as it is what you're trying to do.

you will not achieve positive results trying to inoculate straw w/ multispore.
straw is suitable as a substrate, but not as spawn. and it is prone to contamination.

not sure what a "sterile technique" is, but you wouldn't be able to inoculate w/ a print in open air.

your bag would need a filter of some sort.

Quote:

psilocyben said:
you can always do brf cakes and spawn them to pasturized horse poo.





the best advice i can give you :japsmile:
:zoom:


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InvisibleMrMaddHatter
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4235119 - 05/30/05 02:03 AM (11 years, 10 months ago)

Quote:

Smallworlds said:
Substrate consisting of 50% straw 50% wild bird seed is soaked overnight.

The excess moisture is squeezed out and the bags are filled with moist substrate, then the bags are tied shut.

The bags of substrate are heated to 250F in an oven for an hour to allow the center of the substrate to reach 250F sterilizing temps for at least 20 minutes.





You'll never sterilize wild bird seed like that. You can use the oven to pasteurize stuff, but it takes 2-4 hrs.


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OfflineSmallworlds
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Re: O.K. a couple of questions please... [Re: GNIOM1498]
    #4235128 - 05/30/05 02:06 AM (11 years, 10 months ago)

if the temperature in the oven is 250 then everything in it will become 250 too, given enough time.

I'm not sure I understand what you were trying to say, could you be a little more specific on what you mean by that?

there hasta be moisture in the bags or else the shit wont grow.


?????????????


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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Offlinepsilocyben
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4235132 - 05/30/05 02:08 AM (11 years, 10 months ago)

you need 15 lbs per sq in. to kill the endospores on wbs.
steaming or oven pasturization is not suitable as in brf cakes.

edit: i didn't mean you can sterilize brf jars in the oven, either.
:zoom:


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Edited by psilocyben (05/30/05 02:09 AM)


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OfflineSmallworlds
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Re: O.K. a couple of questions please... [Re: psilocyben]
    #4235137 - 05/30/05 02:09 AM (11 years, 10 months ago)

Quote:

psilocyben said:
it's not so much how you're doing it, as it is what you're trying to do.

you will not achieve positive results trying to inoculate straw w/ multispore.
straw is suitable as a substrate, but not as spawn. and it is prone to contamination.

not sure what a "sterile technique" is, but you wouldn't be able to inoculate w/ a print in open air.

your bag would need a filter of some sort.

Quote:

psilocyben said:
you can always do brf cakes and spawn them to pasturized horse poo.





the best advice i can give you :japsmile:
:zoom:




could I spawn them to the substrate I described and skip the horse shit?


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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OfflineSmallworlds
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Re: O.K. a couple of questions please... [Re: psilocyben]
    #4235163 - 05/30/05 02:15 AM (11 years, 10 months ago)

Quote:

psilocyben said:
you need 15 lbs per sq in. to kill the endospores on wbs.
steaming or oven pasturization is not suitable as in brf cakes.

edit: i didn't mean you can sterilize brf jars in the oven, either.
:zoom: 




you mean if I put those bags in the oven at even 350 degrees they wouldn't become sterile?

I'm missing something here, just because 15psi produces 250 degree temps shouldn't mean that if I produced that temp by some other means, such as in an oven, that it shouldn't sterilize just as well as with the pressure cooker method. I don't see why the pressure cooker method is magic if the same temps can be produced by an oven.

What am I not understanding here?


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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Offlinepsilocyben
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4235165 - 05/30/05 02:16 AM (11 years, 10 months ago)

wbs is not a suitable substrate to spawn to.
straw is o.k. but you will run the risk of contamination.

9 out of 10 dentists reccomend the H poo.
it is very resistant to contaminants, so you don't have to be as careful.
it will produce mushrooms more potent than on any other substrate i know of.
it will give you monster flushes.

a little pic whoring:

if you can't find any: http://www.tennstud.com/page/page/1673025.htm


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Edited by psilocyben (05/30/05 02:18 AM)


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Offlinepsilocyben
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4235187 - 05/30/05 02:20 AM (11 years, 10 months ago)

Quote:

Smallworlds said:
What am I not understanding here?



the part i said about needing 15 psi to kill endospores.
:zoom:

'night guys


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Edited by psilocyben (05/30/05 02:21 AM)


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OfflineSmallworlds
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Re: O.K. a couple of questions please... [Re: psilocyben]
    #4235190 - 05/30/05 02:21 AM (11 years, 10 months ago)

nice!

yeah, I'm just fucking off experimenting anyway. if it don't work I'm out 5 bucks.

once i get the cooker ill be happy, but i'm a fucking tightwad so i'm dragging my feet


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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OfflineSmallworlds
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Registered: 03/12/05
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Re: O.K. a couple of questions please... [Re: psilocyben]
    #4235199 - 05/30/05 02:23 AM (11 years, 10 months ago)

Quote:

psilocyben said:
Quote:

Smallworlds said:
What am I not understanding here?



the part i said about needing 15 psi to kill endospores.
:zoom:




thanks, i'll let u guys know if the shit works against all odds. if it does, you know i'll be braggin'. haha

if not i'm learnin' from the experience at least. thx


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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OfflineIGnosticAbhorI
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Re: O.K. a couple of questions please... [Re: Smallworlds]
    #4235256 - 05/30/05 02:44 AM (11 years, 10 months ago)

Dry spore prints work fine...They just take a bit longer b/c they have to rehydrate...

Grains need to be STERILYZED

Straw needs to be PASTURIZED...

You'd be looking at what Agar would call a Bacterial Bloom

You'd pasturize the straw, but you wouldnt kill endospores...



You should read more, this is already being used in some degree (in regards to oven bags)

Read up and good luck

-Gnostic


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