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Invisiblemyndreach
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Would this be a better environment for incubation?
    #4229883 - 05/28/05 02:07 PM (12 years, 6 months ago)

Currently these hypothetical jars are incubating in a cardboard box that isn't totally sealed shut, and that is 78-82 degrees throughout the day. My friend is worried they will dry out as we live in AZ and it's quite arid here.

Would it be better to put them in a cleaned out Rubbermaid container that can be totally shut with the lid snapped on? They inside walls would be sprayed with a h2o/h2o2 mixture to make it humid inside the chamber. Also, the plastic would probably create a more warm environment.


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Invisibledeanofmean
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Re: Would this be a better environment for incubation? [Re: myndreach]
    #4229905 - 05/28/05 02:13 PM (12 years, 6 months ago)

80% RH is recommended for incubation. :smile:


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Invisiblemyndreach
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Re: Would this be a better environment for incubation? [Re: deanofmean]
    #4229925 - 05/28/05 02:17 PM (12 years, 6 months ago)

Hmm...I don't have a RH indicator and absolutely no money.

I'm sure it's MUCH lower than that. However, isn't 80% where you can just see small beads on the walls of the chamber? If so, the box they are in is dry as a bone lol.

I'll get them into a proper rubbermaid, sprayed-down chamber this evening. I'm heading out now. They showed huge growth that started only 2 days after inoculation and got 30% within 4 days of inoculation, but there has been very little growth in the last 24 hours. I think they've dried out. If they get in a humid environment, they will start growing again, correct?


Edited by myndreach (05/28/05 02:19 PM)


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