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Offlinejtk
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Syringe Steralization
    #4220628 - 05/26/05 12:32 AM (11 years, 6 months ago)

I need to steralize a couple of syringes to suck some LC up into. Can i just boil some water in the Microwave for about 5 min then suck it repeatedly up into the syringe and shoot it out? Maybe even suck up some bleach/water first and slosh it around, then rinse out with boiling water? Does this methodd sound like it would work?


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OfflinekronnyQ
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Re: Syringe Steralization [Re: jtk]
    #4220859 - 05/26/05 01:24 AM (11 years, 6 months ago)

It may work but you are not guaranteed 100%. PC is really the only way to thoroughly and properly sterilize.


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Invisibleagar
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Re: Syringe Steralization [Re: kronnyQ]
    #4220863 - 05/26/05 01:25 AM (11 years, 6 months ago)

^^DITTO^^ :thumbup:


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InvisibleDiploidM
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Re: Syringe Steralization [Re: jtk]
    #4221501 - 05/26/05 07:12 AM (11 years, 6 months ago)

Water in a microwave will not get hotter than boiling at 212F (sometimes it'll superheat a few more degrees) and this isn't hot enough to sterilize effectively.

A pressure cooker nominally reaches 250F to sterilize.


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Offlinebixo
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Re: Syringe Steralization [Re: Diploid]
    #4221508 - 05/26/05 07:18 AM (11 years, 6 months ago)

definitely best to PC, and you really only have to for like 20 mins so its not a big inconvenience... ive heard many say boiling water works fine but its a risk


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Offlinevescudero0
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Re: Syringe Steralization [Re: bixo]
    #4222580 - 05/26/05 02:49 PM (11 years, 6 months ago)

is it ok just to use a brand new out of the plastik wraper syring???
just take the plastik off and make the syring???


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OfflineNiGGy
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Re: Syringe Steralization [Re: vescudero0]
    #4223068 - 05/26/05 04:55 PM (11 years, 6 months ago)

yes, that'd be fine. it's already sterile. :smile:


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Offlinebrewwhaha1
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Re: Syringe Steralization [Re: NiGGy]
    #4223671 - 05/26/05 07:46 PM (11 years, 6 months ago)

brand new syringes are fine. but recycling them is easy on my pocket book. I got a nice big 20 cc with a 14 gauge needle from the TSC (tractor supply store). works great as the neeldle gauge is large enough to allow myc to pass through it without gettign all bunged up.
I sterilize by boiling it in water for 15 min. and then let it cool in aluminum foil b4 using it.


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Invisiblelaurden
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Re: Syringe Steralization [Re: brewwhaha1]
    #4284648 - 06/11/05 10:35 PM (11 years, 5 months ago)

PCing plastic syringes.... what's the chance of these things melting?


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OfflineIGnosticAbhorI
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Re: Syringe Steralization [Re: laurden]
    #4284653 - 06/11/05 10:38 PM (11 years, 5 months ago)


Quote:


what's the chance of these things melting?




No chance. Unless you throw them on a fire.


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Offlineliveby
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Re: Syringe Steralization [Re: IGnosticAbhorI]
    #4284996 - 06/12/05 01:39 AM (11 years, 5 months ago)

120deg is sterilization temp


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OfflineIGnosticAbhorI
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Re: Syringe Steralization [Re: liveby]
    #4285106 - 06/12/05 02:03 AM (11 years, 5 months ago)

Quote:

liveby said:
120deg is sterilization temp




Pasturization is 170F.

You're way off.

-Gnostic


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InvisibleQuiescence
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Re: Syringe Steralization [Re: IGnosticAbhorI]
    #4285176 - 06/12/05 02:15 AM (11 years, 5 months ago)

Well.. 120 celcius would sterilize...


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OfflineIGnosticAbhorI
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Re: Syringe Steralization [Re: Quiescence]
    #4285250 - 06/12/05 02:29 AM (11 years, 5 months ago)

....:smirk:

:thumbup:

-Gnostic


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Offlineblackout
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Re: Syringe Steralization [Re: IGnosticAbhorI]
    #4302552 - 06/16/05 04:55 AM (11 years, 5 months ago)

I have been sterilizing mine with just bleach lately. I dip the syringe needle in bleach, suck up .25cc and pull air in the rest of the way. I just shake the bleach around the syringe and plung up and down a few times. I leave for 20mins and then squirt it all out, wipe the needle with alcohol and stick it in a LC jar. I pull up 12cc from the top with no myc, I squirt this out, and stick it in again and get some proper myc LC the next time.
Discarding the first clear 12cc does 2 things. It dilutes the remaining bleach in the syringe so when it is squirted out it leaves only a bare trace of bleach in the syringe (you could do it 2 or 3 times if worried). It also concentrates the LC by removing clear liquid, it is now easier to suck up a syringe with lots of myc as it is more concentrated.


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Edited by blackout (06/16/05 04:58 AM)


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Amazon Shop for: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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