|
 jtk
Stranger
Registered: 02/06/05
Posts: 618
Last seen: 10 years, 8 months
|
 Syringe Steralization 
#4220628 - 05/26/05 12:32 AM (12 years, 10 months ago) |
|
|
I need to steralize a couple of syringes to suck some LC up into. Can i just boil some water in the Microwave for about 5 min then suck it repeatedly up into the syringe and shoot it out? Maybe even suck up some bleach/water first and slosh it around, then rinse out with boiling water? Does this methodd sound like it would work?
--------------------
Don't let your brain tell your mind it can't feel
|
kronnyQ
SuperstudExtraordinaire
Registered: 07/23/04
Posts: 2,488
Loc: Anytown USA
Last seen: 1 year, 9 months
|
Re: Syringe Steralization [Re: jtk]
#4220859 - 05/26/05 01:24 AM (12 years, 10 months ago) |
|
|
It may work but you are not guaranteed 100%. PC is really the only way to thoroughly and properly sterilize.
|
 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Syringe Steralization [Re: kronnyQ]
#4220863 - 05/26/05 01:25 AM (12 years, 10 months ago) |
|
|
^^DITTO^^ 
--------------------
|
Diploid
Cuban
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
|
 Re: Syringe Steralization [Re: jtk]
#4221501 - 05/26/05 07:12 AM (12 years, 10 months ago) |
|
|
Water in a microwave will not get hotter than boiling at 212F (sometimes it'll superheat a few more degrees) and this isn't hot enough to sterilize effectively.
A pressure cooker nominally reaches 250F to sterilize.
--------------------
Republican Values:
1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.
4) We need a smaller, less-intrusive government.
|
bixo
Dremel Addict
Registered: 02/14/05
Posts: 388
Loc: The Autobahn
Last seen: 5 years, 17 days
|
Re: Syringe Steralization [Re: Diploid]
#4221508 - 05/26/05 07:18 AM (12 years, 10 months ago) |
|
|
definitely best to PC, and you really only have to for like 20 mins so its not a big inconvenience... ive heard many say boiling water works fine but its a risk
|
vescudero0
ay ay aaaaay
Registered: 01/28/05
Posts: 418
Last seen: 11 years, 5 months
|
Re: Syringe Steralization [Re: bixo]
#4222580 - 05/26/05 02:49 PM (12 years, 10 months ago) |
|
|
is it ok just to use a brand new out of the plastik wraper syring???
just take the plastik off and make the syring???
--------------------
live & enjooooooooooooooooy
|
NiGGy
Stranger
Registered: 02/10/02
Posts: 877
Loc: Nova Scotia, Canada
Last seen: 12 years, 1 month
|
Re: Syringe Steralization [Re: vescudero0]
#4223068 - 05/26/05 04:55 PM (12 years, 10 months ago) |
|
|
yes, that'd be fine. it's already sterile. 
|
brewwhaha1
Stranger thanmost
Registered: 02/13/05
Posts: 253
Last seen: 12 years, 4 months
|
Re: Syringe Steralization [Re: NiGGy]
#4223671 - 05/26/05 07:46 PM (12 years, 10 months ago) |
|
|
brand new syringes are fine. but recycling them is easy on my pocket book. I got a nice big 20 cc with a 14 gauge needle from the TSC (tractor supply store). works great as the neeldle gauge is large enough to allow myc to pass through it without gettign all bunged up.
I sterilize by boiling it in water for 15 min. and then let it cool in aluminum foil b4 using it.
|
laurden
ubershroomer
Registered: 04/07/05
Posts: 12
|
Re: Syringe Steralization [Re: brewwhaha1]
#4284648 - 06/11/05 10:35 PM (12 years, 10 months ago) |
|
|
PCing plastic syringes.... what's the chance of these things melting?
|
IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,898
Last seen: 2 years, 5 months
|
Re: Syringe Steralization [Re: laurden]
#4284653 - 06/11/05 10:38 PM (12 years, 10 months ago) |
|
|
Quote:
what's the chance of these things melting?
No chance. Unless you throw them on a fire.
|
liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 7 years, 3 days
|
|
120deg is sterilization temp
--------------------
 
http://www.bruceeisner.com/ -Creating a Sensible Culture
|
IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,898
Last seen: 2 years, 5 months
|
Re: Syringe Steralization [Re: liveby]
#4285106 - 06/12/05 02:03 AM (12 years, 10 months ago) |
|
|
Quote:
liveby said:
120deg is sterilization temp
Pasturization is 170F.
You're way off.
-Gnostic
|
Quiescence
Intriguer
Registered: 04/25/05
Posts: 315
|
|
Well.. 120 celcius would sterilize...
--------------------
God raised up the fellow who was relaxed in the company of tax collectors, prostitutes and other outsiders, and went on to say that many of them would be welcomed to God?s banquet table long before those self-righteous people on display at church.
|
IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,898
Last seen: 2 years, 5 months
|
Re: Syringe Steralization [Re: Quiescence]
#4285250 - 06/12/05 02:29 AM (12 years, 10 months ago) |
|
|
....
-Gnostic
|
blackout
Microwaver
Registered: 07/17/00
Posts: 4,901
Last seen: 19 days, 8 hours
|
|
I have been sterilizing mine with just bleach lately. I dip the syringe needle in bleach, suck up .25cc and pull air in the rest of the way. I just shake the bleach around the syringe and plung up and down a few times. I leave for 20mins and then squirt it all out, wipe the needle with alcohol and stick it in a LC jar. I pull up 12cc from the top with no myc, I squirt this out, and stick it in again and get some proper myc LC the next time.
Discarding the first clear 12cc does 2 things. It dilutes the remaining bleach in the syringe so when it is squirted out it leaves only a bare trace of bleach in the syringe (you could do it 2 or 3 times if worried). It also concentrates the LC by removing clear liquid, it is now easier to suck up a syringe with lots of myc as it is more concentrated.
--------------------
support the free spore ring http://fsre.nl/
Edited by blackout (06/16/05 04:58 AM)
|
|
Threaded
Previous
Index
Next
Shop: 
Edit 
Reply 
