|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 Randolph_Carter
НơĻ?ĢΉōsŧ
Registered: 06/13/00
Posts: 29,281
Loc: Shroomery B-list.
Last seen: 13 years, 4 months
|
 Freezing steriilization? 
#421806 - 10/11/01 09:45 AM (21 years, 11 months ago) |
|
|
Another idea....sadly, i dont have the time, or the environment control to test these ideas effectivly as of yet, but trust you me i will find the area. Does anyone know the temperature ranges of most infectious strains of bacteria? because if you froze a substrate (say, whole grains), it would not only rupture the cells of the substrate making easier availability to nutrients, and kill the evil invaders on the side...opinions? Stay frosty.
--------------------
"..all those molecules thrashing their kinky little tails, hot for destiny and the street." Gibson
Nuke baby seals for Jesus!
(This has been a +1 production.)
|
BeppoMarx
old hand
Registered: 09/01/01
Posts: 1,126
Last seen: 18 years, 5 months
|
|
im no expert by far but i do believe that freezing most mold spores and bacteria will only render them inactive for the time that they are frozen. im pretty sure once they warm back up they will just go about their way. the only way I could see this working is by a rapid and large temperature decrease such as using liquid nitrogen or possibly dry ice (solidified CO2). but putting them in the freezer will gently cool the contams into a dormant but still potentaily hazardus state. try it though, i may be wrong. i personally wouldnt waste my spores or limited supply of verm. to test it tho.
--------------------
Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!
|
honeyroasted
member
Registered: 06/30/01
Posts: 125
Last seen: 21 years, 4 months
|
Re: Freezing steriilization? [Re: BeppoMarx]
#421864 - 10/11/01 10:58 AM (21 years, 11 months ago) |
|
|
in all likelyhood it would require extreme cold temps....haven't they found bacteria living under hundreds of feet of antarctic ice? it's damn cold down there, for sure...
|
shellacct
addict
Registered: 02/01/01
Posts: 427
Loc: BAD DOG!
Last seen: 1 month, 20 days
|
|
the biggest problem with this is that when water freezes IT EXPANDS.... in plain english, the jars will crack and/or explode if you tried this. And besides, it would have to be mighty fucking cold to sterilize by freezing.... i'm talking close to absolute zero.
"Trying is the first step towards failure" -Homer J. Simpson
In the above, Any and all references to reality are entirely fictional.
Just because you are paranoid, don't mean they're not after you...
--------------------
"Trying is the first step towards failure" -Homer J. Simpson
In the above, Any and all references to reality are entirely fictional.
|
heathadelic
member
Registered: 07/24/01
Posts: 159
Last seen: 21 years, 7 months
|
Re: Freezing steriilization? [Re: BeppoMarx]
#421947 - 10/11/01 12:16 PM (21 years, 11 months ago) |
|
|
Even dunking in liquid nitrogen will not kill bacteria. In fact rapid freezing helps them to survive the process. We use this method in our lab to keep bacteria stocks for years.
Swing the groove 'round here, so I can reach it -a.d.
--------------------
"...time is not money, time is an opportunity to live before you die."
|
Randolph_Carter
НơĻ?ĢΉōsŧ
Registered: 06/13/00
Posts: 29,281
Loc: Shroomery B-list.
Last seen: 13 years, 4 months
|
Re: Freezing steriilization? [Re: shellacct]
#422324 - 10/11/01 07:11 PM (21 years, 11 months ago) |
|
|
Two clarifications:
A: You're asuming i'm using jars....never liked the things anyways.
B: I concede the point on bacteria killing...shoulda thought first, but the first idea about the cellular breakdown of the substrate seems sound. Just throwing out ideas tho, its good to see someone else is thinking on the subject...stay frosty.
--------------------
"..all those molecules thrashing their kinky little tails, hot for destiny and the street." Gibson
Nuke baby seals for Jesus!
(This has been a +1 production.)
|
mew
member
Registered: 05/09/01
Posts: 151
Last seen: 20 years, 11 months
|
|
if ya froze em in any way it would prolly act like cryogenics an theyed stay dormant till they found a hospitable environment to wreack their havoc in fact i read some where that zero temps will acctually help as in preserve the various spores for long term dormancy so for keeping a vialable strain via spores cryogenics would be the way to ensure their survival .Mew
support the FSR
--------------------
support the FSR
|
MAIA
World-BridgerKartikeya (DftS)
 
Registered: 04/27/01
Posts: 7,391
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 days, 4 hours
|
|
I used to move my jars to a big (-30?C) freezer right after they have been PCed and let them rest all night inside it.
Conclusions.
- I never had a single contam this way.
- Had pressure problems inside the jars after defreeze them.
- Putting hot things inside the freezer wrecks it (I didn't mind).
Peace,
MAIA
"Houston, we have a problem... shrooms are over "
=)Free&Spread the Spores(=
Support the FSR
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
|
|
|
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,022 topic views. 20 members, 107 guests and 19 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|
Threaded
Previous
Index
Next
Buy Super Blended Green Sumatra 
Unfolding Nature: Being in the Implicate Order 
Substrate Bags 
High THC Strains 
Buy Kratom Extract 
Bulk Substrate 
Red Vein Kratom
Edit 
Reply 
Ru
1 2 all )
GaNjAShRooM
GNIOM1498
IGnosticAbhorI
novicemycology
Tylor