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OfflineMych
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Registered: 03/25/05
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Last seen: 10 years, 10 months
3/4 strains = no go?
    #4209464 - 05/23/05 11:35 AM (11 years, 9 months ago)

i got 4 strains from TLG: Ecuador, Cambodian, Thai KS Super Strain, and they sent a free Golden Teacher.

I made 8 jars of BRF jars by following PF Tek (2 jars of each strain), and 4 jars of Karo (1 jar of each strain). PF jars were knocked up on the 11th of this month. One of GT jars started showing some life exactly 1 week ago. We are now 12 days after inoculation and i have my ALL my GT jars showing life and NOTHING from the other jars.

whats the deal here? i am completely new to this but 3/3 GT, 0/3 CAM, 0/3 EQ, 0/3 KSSS... its kinda hard to see the pattern. bad syringes??

and for your time, a new piece i picked up:




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OfflinekronnyQ
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Re: 3/4 strains = no go? [Re: Mych]
    #4209474 - 05/23/05 11:40 AM (11 years, 9 months ago)

Are you incubating? Can we see a pic of one of your jars?


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OfflineMycoJunkie
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Registered: 11/04/04
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Re: 3/4 strains = no go? [Re: kronnyQ]
    #4209483 - 05/23/05 11:42 AM (11 years, 9 months ago)

Damn, nice pipe. I'd wait another week. If still nothing happens, I'd open 1 of the jars and investigate. Maybe its too watery or not enough, or the kernals arent popped enough or something. What tek did you use?

Those are great strains btw


--------------------
:cussing::whip:


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OfflineMych
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Re: 3/4 strains = no go? [Re: kronnyQ]
    #4209496 - 05/23/05 11:48 AM (11 years, 9 months ago)

Quote:

kronnyQ said:
Are you incubating? Can we see a pic of one of your jars?




yes sorry. ive been incubating in a TiT.. ive kept the temps in the 79-85 range (stupid heater theromstats arent very accurate). I don't have current pics of the jars on hand but ive basically got a 1" splotch of mych and a few other .5-1cm splotches around the PF jars and some nice slimey mych in the Karo.. as for the jars doing nothin.. well they look just the way they did when they came out of the pressure cooker except for the EQ jars... they both have a dark stain on the BRF at the innoc points that showed up immediately after shooting them up. I was told this is most likely due to some jet dry that was used to make the syringe.

I could get some pics later tonight if needed.


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OfflineMych
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Re: 3/4 strains = no go? [Re: MycoJunkie]
    #4209499 - 05/23/05 11:49 AM (11 years, 9 months ago)

Quote:

MycoJunkie said:
Damn, nice pipe. I'd wait another week. If still nothing happens, I'd open 1 of the jars and investigate. Maybe its too watery or not enough, or the kernals arent popped enough or something. What tek did you use?

Those are great strains btw




basic PF Tek... All the jars came from the same batch, so why one strain would take off 100% while the others were 0% doesnt make sense. Granted i have never done this before.. but thats why im asking wtf :P


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Invisibledeathbychimney
45th GenerationRoman
Registered: 02/26/05
Posts: 239
Re: 3/4 strains = no go? [Re: Mych]
    #4209537 - 05/23/05 12:05 PM (11 years, 9 months ago)

how long was it inbetween PC and inoculation?
did you shake the syringes?


--------------------
diarrhea takes over your life


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Invisibledeathbychimney
45th GenerationRoman
Registered: 02/26/05
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Re: 3/4 strains = no g [Re: Mych]
    #4209539 - 05/23/05 12:06 PM (11 years, 9 months ago)

double post :frown:


--------------------
diarrhea takes over your life


Edited by deathbychimney (05/23/05 12:06 PM)


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OfflineMych
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Re: 3/4 strains = no go? [Re: deathbychimney]
    #4209541 - 05/23/05 12:07 PM (11 years, 9 months ago)

Quote:

deathbychimney said:
how long was it inbetween PC and inoculation?
did you shake the syringes?




syringes were shaken quite well with the existing airbubble in it. was about 24 hours between PC and innoc.


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OfflineMych
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Re: 3/4 strains = no go? [Re: Mych]
    #4210139 - 05/23/05 03:14 PM (11 years, 9 months ago)

any ideas?


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Offlinebrewwhaha1
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Re: 3/4 strains = no go? [Re: Mych]
    #4210759 - 05/23/05 05:55 PM (11 years, 9 months ago)

there are a number of possibilities. Are your jars getting any o2??
if you have taped the holes shut it will prevent growth. perhaps your substrate is too wet or too dry. or worse yet maybe you have three bad syringes. do you have good growth in the jars that are colonizing or is it really slow??? if its slow i would guess you have a moisutre problem. if its taken off really quick and the hole jars are colonizing its most likly your syringes.


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OfflineMych
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Re: 3/4 strains = no go? [Re: brewwhaha1]
    #4210831 - 05/23/05 06:11 PM (11 years, 9 months ago)

Quote:

brewwhaha1 said:
there are a number of possibilities. Are your jars getting any o2??
if you have taped the holes shut it will prevent growth. perhaps your substrate is too wet or too dry. or worse yet maybe you have three bad syringes. do you have good growth in the jars that are colonizing or is it really slow??? if its slow i would guess you have a moisutre problem. if its taken off really quick and the hole jars are colonizing its most likly your syringes.




the holes on the jars are open. I may very well have a moisture issue has the GT jars are going kinda slowish.. but what about the Karo? kinda hard to fuck up that one.


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InvisibleShnezbit
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Re: 3/4 strains = no go? [Re: Mych]
    #4210868 - 05/23/05 06:18 PM (11 years, 9 months ago)

i have had jars not colonize im pretty sure because i packed the substrate down. i learned not to put the full amount in a jar so i dont need to pack any down. not saying this is what happened to u, but just mentioning it as a possible cause.
o yea...the little guy rules imho. their syringes are pretty reliable. but im sure if u narrow it down to being the syringes and write them they'll work something out with u, they're good like that.


Edited by Shnezbit (05/23/05 06:21 PM)


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OfflineMych
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Re: 3/4 strains = no go? [Re: Shnezbit]
    #4210940 - 05/23/05 06:33 PM (11 years, 9 months ago)

well i have about 9cc left in each of the 3 "failed" syringes.. should i try to do some karo jars? more PF cakes?


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OfflineGatorB
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Re: 3/4 strains = no go? [Re: Mych]
    #4210952 - 05/23/05 06:39 PM (11 years, 9 months ago)

Screw the PF cakes, get some WBS at Wal-Mart and some horse poo from a local farm. Much easier.


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OfflineMych
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Re: 3/4 strains = no go? [Re: GatorB]
    #4211027 - 05/23/05 07:01 PM (11 years, 9 months ago)

Quote:

GatorB said:
Screw the PF cakes, get some WBS at Wal-Mart and some horse poo from a local farm. Much easier.




well i have some rye berries... i knocked up 1 jar each from the karo jars i have just to see if i could somehow bring a minimal ammount of mych that might be there "alive". shall i just start shooting some spores into rye jars?
i might order some poo from tennstud but i think ill just go straight to verm casing for the first few tries. I was wanting to get some of the dried poo from them but i couldnt find the info on how to utilize dried stuff yet.


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