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Amazon Shop: ½ Pint Jars, Agar

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OfflineHidingInPlainSight
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myc isolation through LC
    #4209408 - 05/23/05 11:07 AM (12 years, 10 months ago)

Is it possible to isolate healthy mycelium, then transfer it to a new jar to grow contam free (if the previous jar had a contaminant in it)?


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Offlinescarymidgets
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Re: myc isolation through LC [Re: HidingInPlainSight]
    #4209438 - 05/23/05 11:20 AM (12 years, 10 months ago)

i tried it ended up with a couple LC jars every thing looked ok
then i shot up some jars . all contams


you can try tho but i wouldnt rely on it workin out


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OfflineHidingInPlainSight
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Re: myc isolation through LC [Re: scarymidgets]
    #4209449 - 05/23/05 11:25 AM (12 years, 10 months ago)

I think a way to simplify it would be to take the LC that got contaminated and:

make around four or five 60ml LC's
take mycelium from one spot, shoot into a small jar.
clean the syringe with alcohol, and draw in boiling water, let it sit, then taking another small sample.
repeat the process for the other small jars.

out of 4 or 5 jars you probably would have a successful one then transfer that to a bigger jar or grow it out on grain.


Edited by HidingInPlainSight (05/23/05 11:25 AM)


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Offlinescarymidgets
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Re: myc isolation through LC [Re: HidingInPlainSight]
    #4209476 - 05/23/05 11:40 AM (12 years, 10 months ago)

the whole spinning the jars for aeration wouldn't that just mix all the different mycelium up? I thought agar isolation only worked because the contaminates were for the most part localized so you can exploite cubi mycelium natural defense against foreign species

also transfers workt to isolate mycelium from contaminate spores usein h202 least that's how i understood it but then I'm probable wrong


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PLs People this a drug info site some people may be on drugs. /flameing might make you feel smart but makes you look dumb.


cant stop me if i eat your soul.


Edited by scarymidgets (05/23/05 11:42 AM)


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OfflineIGnosticAbhorI
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Re: myc isolation through LC [Re: HidingInPlainSight]
    #4209481 - 05/23/05 11:41 AM (12 years, 10 months ago)

Quote:

i tried it ended up with a couple LC jars every thing looked ok
then i shot up some jars . all contams




Had the same thing happen to me. At some point you introduced Contams., Meanie greenie got me, but it didn't turn green until After I knocked up all my jars, the green is white in it's early stages...

But if Could work

-Gnostic


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Amazon Shop: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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