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Amazon Shop for: ½ Pint Jars, Agar

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OfflineSouthPArk
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    #4189064 - 05/17/05 11:42 PM (11 years, 9 months ago)

.


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Live as if you were to die tomorrow. Learn as if you were to live forever.


Edited by SouthPArk (04/18/08 07:19 PM)


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OfflineSammy
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Re: When to Shake?? [Re: SouthPArk]
    #4189080 - 05/17/05 11:45 PM (11 years, 9 months ago)

Shake when it cools down to dissipate h2o better within the jar, then inoculate. A few days after inoculation - a few more, you might want to shake again (when the mycelium eats about 10-20% of the jar. Some shake only once, some shake again when the jars are more colonized. Don't shake when they're close to being done.

Sam


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OfflineSouthPArk
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Re: When to Shake?? [Re: Sammy]
    #4189087 - 05/17/05 11:48 PM (11 years, 9 months ago)

So the main thing is.....

Shake to Speed up Coloniziung but Dont shake after 80%?

What about right after the knockup?

Some people say Shack it like a Crazed nut and other they dont!
Would their be a big differnece from being shaken at inoc??


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OfflineSammy
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Re: When to Shake?? [Re: SouthPArk]
    #4189100 - 05/17/05 11:53 PM (11 years, 9 months ago)

Southpark. .. maybe?? I might venture a guess that if you don't shake immediately after inoculation (I'd use a liquid culture) then the mycelium growth would be centralized around these areas.. allowing lots of mycelium to grow quickly in those places. If you shook it all up right after innoc, you would disperse all the LC or Spore Solution, causing a slower start.

So, to recap. PC, then shake to distribute the H2O. inoculate, then wait for some growth. Shake (VERY WELL) to distribute. Wait a bit and shake again if you desire. More shaking doesn't always = faster colonization.

Sam


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Invisibleagar
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Re: When to Shake?? [Re: SouthPArk]
    #4189543 - 05/18/05 02:43 AM (11 years, 9 months ago)

Shake hot out of the PC (small inflated rubber tire does wonders to knock against).

Shake before inoculation.

Don't shake right after inoculation.

Shake around 20% colonization.

Don't shake no more.

If you don't understand why that "timing" is effective. Ponder this:

Shaking batters & injures Myc.

It takes time to heal, (2 OR 3 DAYS) before it can fully rejuvinate, grow & spread again.

Consiquently, repeated shaking slows things - down.

This method works for me.

Do it - your way & one this way & compare the 2.

I did.


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OfflineAnnoA
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Re: When to Shake?? [Re: SouthPArk]
    #4189625 - 05/18/05 03:19 AM (11 years, 9 months ago)

Quote:

SouthPArk said:
-Shake when it Cools down a little to prevent CLumping La8r!
-Shake right after inoculation
-shake after 20% colonization?




That's basically what I do.


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InvisibleGnuBobo
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Re: When to Shake?? [Re: agar]
    #4189632 - 05/18/05 03:24 AM (11 years, 9 months ago)

Quote:

agar said:
Shake hot out

Don't shake no more.

This method works for me.

I did.





OMG!  Agar = Tina Turner! 

NM.  :smirk:  Thanks for the advice all.  :cool:


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InvisibleRoadkill
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Re: When to Shake?? [Re: SouthPArk]
    #4189645 - 05/18/05 03:29 AM (11 years, 9 months ago)

what I do...

Shake hot out of the PC

Shake before inoculation.

Shake right after inoculation.

Shake around 20% colonization.

Don't shake no more.

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisibleagar
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Re: When to Shake?? [Re: Roadkill]
    #4190376 - 05/18/05 11:52 AM (11 years, 9 months ago)



I inoculate 5 squirts, 1 down the center, 1 in all four corners, against the glass. I don't shake right after inoculation, because I want to see what is going on against the glass.

The jar above is just such an example.

You can SEE the inoculation points CLEARY.

But.....hey.... it all works... no matter which way.

So long as you inject some VIABLE spores.


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Amazon Shop for: ½ Pint Jars, Agar

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