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OfflineOwlofMinerva
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Registered: 04/08/05
Posts: 9
Last seen: 11 years, 2 months
Never inoculate again?
    #4183345 - 05/16/05 07:35 PM (11 years, 8 months ago)

Is it possible to just keep the mycelia growing out indefinetly? Let's say I have a half pint of mycelia on BRF/Verm. Could I just transfer this in a 30% spawn to 70%substrate ratio onto WBS. Then once that colonizes, use some of it to colonize others and do this until the end of time? Of course I would be fruiting most of the colonized substrate.


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Offlinelordoftheshroomz
recoveringaddict
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Posts: 1,631
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Last seen: 9 years, 9 months
Re: Never inoculate again? [Re: OwlofMinerva]
    #4183350 - 05/16/05 07:36 PM (11 years, 8 months ago)

this is called a grain to grain transfer and u wouldnt want to continue it indefinately because the genetics or something wears out making for not as good shrooms


--------------------
MY Glove Box, Automated Terrarium, and Incubator
Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest.
THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!


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Offlinelordoftheshroomz
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Re: Never inoculate again? [Re: lordoftheshroomz]
    #4183387 - 05/16/05 07:43 PM (11 years, 8 months ago)

oh and it is also a type of inoculation, wut u mean is never INJECT again


--------------------
MY Glove Box, Automated Terrarium, and Incubator
Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest.
THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!


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Offlinecubicidal
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Registered: 03/23/05
Posts: 217
Last seen: 4 years, 8 months
Re: Never inoculate again? *DELETED* [Re: lordoftheshroomz]
    #4185693 - 05/17/05 10:39 AM (11 years, 8 months ago)

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Trusted Cultivator
Re: Never inoculate again? [Re: cubicidal]
    #4186042 - 05/17/05 12:45 PM (11 years, 8 months ago)

What you do is take the BRF jar (generation 1) and split that to 4-6 quarts of WBS, equally (gen 2). Then take ONE of those jars and split that into 12-24 quarts of WBS, equally (gen 3) and fruit the other 3-5 jars of generation 2 mycelium. And then you'll have 12-24 jars of gen 3 mycelium. Dont carry it beyond this point...case ALL of those jars, and start a new one from spores. If you start a new one from spores, when you do that 12-24 gen 3 jars, the one from spores will be ready at that time to go and you can start over. You can take the 1 jar from spores (gen 1), and split that into 12-24 jars of generation 2 WBS (when I'm talking jars of WBS, I'm talking quarts by the way). Then split 1 into 12-24 jars, and fruit the other 11-23 jars and start a new one from spores. This will keep your mycelium fresh, but stretch it out. You can go more transfers but you get slow growth, mutants, and contams..and if you do it, you'll end up screwing yourself if you try and get to many jars that way...just keep cycling, 1cc of spores into a jar, split that jar equally into jars, then split one of those equally into new jars and start a new jar from spores. 1 cc of spores turns into 24-50 jars easy.



--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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