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InvisibleArp
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Cubensis strains & rhizo growth
    #4178211 - 05/15/05 02:13 PM (11 years, 11 months ago)



This is the Gulf Coast. It was hard as hell to get a clean rhizomorphic growth from multispores.
I transfered it several times and there was still fuzz.

These dishes are with a cloned mushroom of Golf Coast and as
you can see there is again fuzzzzz. I'll have to do more transfers.



This one is the Burma! It went rhizo almost instantly and only
one transfer was sufficent from multispore.
These petris are clones and as you can see instant rhizo with
very little fuzz!

The Matias Romero I got was a hell, I tried and tried but could
get no decent rhizos out of it. Amazonian was so-so.

Maybe on a different medias & temperatures I would get different results?


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InvisibleMagashM
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Re: Cubensis strains & rhizo growth [Re: Arp]
    #4178520 - 05/15/05 03:30 PM (11 years, 11 months ago)

Good work. I love the GC strain. My personal fav.


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


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Offlinemtr1514
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Re: Cubensis strains & rhizo growth [Re: Magash]
    #4180603 - 05/16/05 01:01 AM (11 years, 11 months ago)

Damn that Burma myc is beautiful. Thanks for the great pix.


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OfflineBlue Helix
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Re: Cubensis strains & rhizo growth [Re: mtr1514]
    #4180709 - 05/16/05 01:28 AM (11 years, 11 months ago)

Your results are interesting, but I have yet to see any conclusive evidence that rhizomorphs at the agar stage really translate to higher yields. A lot of folks are saying that, but I have never seen a shred of evidence.


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OfflineIGnosticAbhorI
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Re: Cubensis strains & rhizo growth [Re: Blue Helix]
    #4180713 - 05/16/05 01:30 AM (11 years, 11 months ago)

Quote:


Maybe on a different medias & temperatures I would get different results?




I don't see why not..

Gl

-Gnostic


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Invisibletahoe
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Re: Cubensis strains & rhizo growth [Re: Arp]
    #4180731 - 05/16/05 01:37 AM (11 years, 11 months ago)

Quote:

Arp said:
.

These dishes are with a cloned mushroom of Golf Coast and as
you can see there is again fuzzzzz. I'll have to do more transfers.







What do you plan on occomplishing by making transfers with a clone. Its the same mycelium, it doesnt get stronger. Clone=clone


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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OfflineIGnosticAbhorI
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Re: Cubensis strains & rhizo growth [Re: tahoe]
    #4180741 - 05/16/05 01:40 AM (11 years, 11 months ago)

Isn't the point of that to isolate a strain....You take the best part (rhizo's) and transfer to agar or whatnot and then use them to knock up bags of spawn...Least that's what I thought....

I thought it was just to make sub. colonize faster....

-Gnostic


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Invisibletahoe
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Re: Cubensis strains & rhizo growth [Re: IGnosticAbhorI]
    #4180758 - 05/16/05 01:48 AM (11 years, 11 months ago)

nostic,
yeah thats the point with multispore. You want to get a good rhizomorphic strain, rhizos for pins and grow through substrate faster. As for cloning, all the isolation has been done for you by nature. There is just one pair of compatible genes in a fruit, you cant change it.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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InvisibleArp
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Re: Cubensis strains & rhizo growth [Re: tahoe]
    #4180889 - 05/16/05 02:32 AM (11 years, 11 months ago)

So with transfers should an isolate be able to turn rhizo or
is this particular Gulf Coast mycel fluffy by nature and prefers
to be that way? It's way slower growing than the Burma. Perhaps
it has other preferences, im gonna get some other medias and try :smile:


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OfflineIGnosticAbhorI
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Re: Cubensis strains & rhizo growth [Re: tahoe]
    #4181148 - 05/16/05 04:13 AM (11 years, 11 months ago)

Ok good, that's what I thought...

When I get my hands on some Agar :grin:

Looks like fun

-Gnostic


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Invisibledeanofmean
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Re: Cubensis strains & rhizo growth [Re: Arp]
    #4181197 - 05/16/05 05:03 AM (11 years, 11 months ago)

rhizomorphs are over rated IMO.
but,if U want a challenge, try it w/ allen strain, or pr. 

nice work you have there though. :cool:


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Invisibleohmatic
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Re: Cubensis strains & rhizo growth [Re: tahoe]
    #4181352 - 05/16/05 07:44 AM (11 years, 11 months ago)

Quote:

tahoe said:
nostic,
yeah thats the point with multispore. You want to get a good rhizomorphic strain, rhizos for pins and grow through substrate faster. As for cloning, all the isolation has been done for you by nature. There is just one pair of compatible genes in a fruit, you cant change it.




ABSOLUTELY NOT RIGHT.
after having extracted various tissues onto agar,
many of us will hav enotices some parts shooting off rhizos,
some shooting of tomentose and alike.

the idea on transferring when cloning is basically the same as with multispore,
extracting the best sectors and again, it is not correct what you say,
as i have numerously seen both types of growth shoot from the same piece of tissue.

see the rhizos starting in the lower right?

peace ohm :mushrooom:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (05/16/05 07:44 AM)


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OfflineIGnosticAbhorI
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Re: Cubensis strains & rhizo growth [Re: ohmatic]
    #4181393 - 05/16/05 08:54 AM (11 years, 11 months ago)

I don't think that's what Tahoe intented to...mean...

When you say cloning, are you sectoring and Isolating the mycelium...Or just making one clone and then using that..

If you being to sector out good pieces (rhizo's), Then wouldn't that basically be Isolating the strain?

Havent seen you post in awhile Ohmatic, Welcome back :shroomer:

-Gnostic


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OfflineIGnosticAbhorI
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Re: Cubensis strains & rhizo growth [Re: deanofmean]
    #4181398 - 05/16/05 09:02 AM (11 years, 11 months ago)

Quote:

deanofmean said:
rhizomorphs are over rated IMO.
but,if U want a challenge, try it w/ allen strain, or pr. 

nice work you have there though. :cool:




I Kinda Agree and disagree....

Fluffy myc. is always a good sign, it lets you know that your spores germ.-ed

Rhizomorphic growth on the other hand cuts through substrate like nothing and is more likely to defeat contam.s....Plus, since it colonizes quickly it lowers contam. rate more, b/c it gives them less time to take over...:cheers:

I wouldn't go to such lengths as some people would, but I do like to see it!
-Gnostic


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Invisibleohmatic
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Re: Cubensis strains & rhizo growth [Re: IGnosticAbhorI]
    #4181419 - 05/16/05 09:15 AM (11 years, 11 months ago)

Quote:

IGnosticAbhorI said:
If you being to sector out good pieces (rhizo's), Then wouldn't that basically be Isolating the strain?

Havent seen you post in awhile Ohmatic, Welcome back :shroomer:

-Gnostic




well the term isolation wouldnt really be right b/c when you work using
extracted tissue you already GOT an isolate, namely the genetics of the cloned mushroom itself.



basically, if you look in the pic above, i sectored out the rhizo part,
transferred to a new plate et voila as you can see its characteristiks
were spread on the dish instead of its tomentose alikes.

oh and btw, being home rules :crazyeyes:
peace ohm :mushroom2:

*edit*
Quote:

Rhizomorphic growth on the other hand cuts through substrate like nothing and is more likely to defeat contam.s....Plus, since it colonizes quickly it lowers contam. rate more, b/c it gives them less time to take over...:cheers:




yeah rhizo myc talking cubensis is kind of "better" in terms of
"fruitability" (lol) and overall colonisation speed and health.

a funny thing is, whever doint multispore jars youll have tons of cottony
shit but sooooome mad rhizos all over the place aswell =)
looking daaamn tight heh -> prove of overall different genetics due to mutlispore.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (05/16/05 09:17 AM)


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