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Offlinelepiota
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liquid culture question
    #4177777 - 05/15/05 12:36 PM (12 years, 14 days ago)

i'm going to make a liquid culture with the baby bottle method, or maybe a jar, still don't know... anyway, i'm going to use mineral water and fructose, please somebody tell me:
1) how many grams of fructose for any 100ml of water?
2) how long should it stay in the PC? (please note that my PC has no gauge, so i don't know how may PSI it reaches)
3) how many ml of spore solution for any 100ml of liquid?


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Edited by lepiota (05/15/05 12:45 PM)


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OfflineHippieChick
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Re: liquid culture question [Re: lepiota]
    #4177878 - 05/15/05 12:54 PM (12 years, 14 days ago)

4% sugar is a pretty good practice. Never used fructose though, use dextrose and malt. One Tablespoon each per quart water. If you can't find these, why not go with a good honey or Karo TEK? Good Luck.
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Edited by HippieChick (05/15/05 12:58 PM)


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Invisiblepoboy
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Re: liquid culture question *DELETED* [Re: HippieChick]
    #4177906 - 05/15/05 01:04 PM (12 years, 14 days ago)

Post deleted by poboy

Reason for deletion: d



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Offlinelepiota
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Re: liquid culture question [Re: poboy]
    #4177967 - 05/15/05 01:18 PM (12 years, 14 days ago)

searched the past posts and found that fructose is as good as dextrose, karo is not sold in the place where i live, honey is sold, but i have fructose at home already so i'll first try it, and move to honey if i fail!
by the way, how much time in PC? i've read 15 minutes, but it refers to a 15 psi cooker, i don' know how many psi is mine, probably much less than that


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Invisiblebackupwards
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Re: liquid culture question [Re: poboy]
    #4177984 - 05/15/05 01:21 PM (12 years, 14 days ago)

agar's bb tek says: 200 mls water and 6 mls karo syrup gives you about a 3% solution. take it from the man.  :eek: missed your last post sorry.


Edited by backupwards (05/15/05 01:23 PM)


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Invisiblebackupwards
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Re: liquid culture question [Re: backupwards]
    #4178001 - 05/15/05 01:24 PM (12 years, 14 days ago)

do you have the thread for that post on fructose?


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Offlinelepiota
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Re: liquid culture question [Re: backupwards]
    #4178082 - 05/15/05 01:39 PM (12 years, 14 days ago)

of course:
http://www.shroomery.org/forums/showflat.php/Number/3323061
scatman's word is THE WORD for me :-)


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Amazon Shop: ½ Pint Jars, Agar, Pressure Cooker

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