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Amazon Shop for: ½ Pint Jars, Pressure Cooker

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OfflineStrandedVoyager
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Keeping Substrate in preperation for inocculation?
    #4175803 - 05/14/05 08:39 PM (11 years, 6 months ago)

Hey, I have 4 spore syringes on the way and my pressure cooker only holds 5 pint jars at a time. With the way all the teks recommend letting jars with substrate cool in a PC overnight before I was wondering if I shouldn't start making and PC'ing substrate now in preperation for when the syringes show up that way I have several jars with sterile substrate ready to go when the syringes ready to go instead of only being able to knock up five jars a day.

I plan to spend the next few days PC'ing substrate and then taping up the holes and storing jars in the fridge. Is that cool?


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OfflineHippieChick
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Re: Keeping Substrate in preperation for inocculation? [Re: StrandedVoyager]
    #4175809 - 05/14/05 08:42 PM (11 years, 6 months ago)

Should be fine. Jars can last for awhile. I've used some after 3 weeks with no problems.
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OfflineMikeOLogical
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Re: Keeping Substrate in preperation for inocculation? [Re: HippieChick]
    #4176287 - 05/14/05 11:10 PM (11 years, 6 months ago)

you don't want to go longer than a few days unless you take some measures to make sure your jars don't dry out... freshly cooked substrate works best...


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InvisibleColonel Kurtz Ph.D
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Re: Keeping Substrate in preperation for inocculation? [Re: HippieChick]
    #4176344 - 05/14/05 11:26 PM (11 years, 6 months ago)

Yes, it will be fine.

However, even after PCing a jar some endospores can survive, but by PCing we get some more time for our spores to germinate over the "bad guys" (you can check this by letting a "control" jar without inoculating it, most probably in a few weeks something will grow on it, at least IME). So you have let's say, two weeks of advantage over contams :thumbup:

So, yeah it's safe. But why not just inoculate 5 jars a day? just 1 day won't make a noticeable difference in colonization times... Unless you're planning to inoc all those 4 syringes directly in which case I strongly recommend for you to prepare some jars in advance :grin:

All in all, it's absolutely safe as long as it doesn't take too long from sterilization to inoculation :thumbup:

I wish you best of luck my friend :mushroom2: :mushroom2:


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InvisibleColonel Kurtz Ph.D
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Re: Keeping Substrate in preperation for inocculation? [Re: MikeOLogical]
    #4176354 - 05/14/05 11:27 PM (11 years, 6 months ago)

Quote:

MikeOLogical said:
you don't want to go longer than a few days unless you take some measures to make sure your jars don't dry out... freshly cooked substrate works best...




Another important fact indeed :smirk:


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OfflineStrandedVoyager
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Re: Keeping Substrate in preperation for inocculation? [Re: Colonel Kurtz Ph.D]
    #4176462 - 05/15/05 12:01 AM (11 years, 6 months ago)

Thank you very much I know I'm asking a lot of annoying questions but I'm trying to be as thorough and clear as possible in my understanding of all this. I think I'll go with doing it five a day when my syringes arrive that way I'll have the best substrate and it'll save me some money in the long run. I still have to buy more jars since I only have a dozen and the spores broke me for this week haha.

I'm getting 48 CCs of spores, 24 of which are already committed to the dozen pint jars I already have. Which means I either need 12 more pint jars or 24 more 1/2 pint jars. I think I'll stick with pint jars for size issues... sorry just talking to myself.

Thanks guys, you're really helping me out :smile:


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OfflineSnarp
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Re: Keeping Substrate in preperation for inocculation? [Re: StrandedVoyager]
    #4176525 - 05/15/05 12:26 AM (11 years, 6 months ago)

There's no need to leave them in the PC overnight, and you can store them for weeks or months without any problems. A sealed jar will not contaminate. Cling wrap over the filter will nearly stop drying.


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Amazon Shop for: ½ Pint Jars, Pressure Cooker

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