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Offlinehardwaters
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Registered: 04/11/05
Posts: 137
Loc: USA
Last seen: 11 years, 1 month
inoculation
    #4170667 - 05/13/05 01:59 PM (12 years, 7 months ago)

Hi,,
I inoculated my jars a week ago last Sunday with BHT strain. I put the needle all the way into the substrate and around the edges. Placed the jars in the incubator, the temp is about 78-80 degrees. Still there is nothing happening. At least I don't have any mold either. Does this strain take longer than a week for me to some progress?

Thanks for your help,


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
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Re: inoculation [Re: hardwaters]
    #4170682 - 05/13/05 02:02 PM (12 years, 7 months ago)

you probably should have seen some type of growth by now.

source of syringes?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinescatmanrav
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Registered: 05/08/04
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Re: inoculation [Re: Roadkill]
    #4170726 - 05/13/05 02:10 PM (12 years, 7 months ago)

Look a little wet or dry? Often water content stalls germination..can take another week or more to germinate...but theres a problem somwhere if its more then 3-5 days IMO.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinethenewguy05
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Re: inoculation [Re: scatmanrav]
    #4170757 - 05/13/05 02:18 PM (12 years, 7 months ago)

did you shake the syringe or did you fire blanks into the cake???


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InvisibleFizikz
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Re: inoculation [Re: scatmanrav]
    #4170760 - 05/13/05 02:19 PM (12 years, 7 months ago)

Raise the temps a little bit try to get it up to 85, you should be ok if you didnt get the substrate to wet. Remember moist, not wet.


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Offlinehardwaters
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Re: inoculation [Re: Roadkill]
    #4170946 - 05/13/05 03:20 PM (12 years, 7 months ago)

Spores101.com


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OfflineCLUTCH
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Re: inoculation [Re: Fizikz]
    #4170953 - 05/13/05 03:22 PM (12 years, 7 months ago)

Yea, what they said. But it would also help if you tell us all the steps you took when innoculating.

One other thing that was not mentioned is the temps of your jars and your needle when you inoculated. If either of them were not at room temperature, it could have killed the spores.

Good luck!


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InvisibleTien
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Re: inoculation [Re: CLUTCH]
    #4171066 - 05/13/05 03:46 PM (12 years, 7 months ago)

I say don't worry about man...it's all about patience.

I had a pf jar of Tazmanians showing growth 4 days after inoculation, and another showed growth after 1 and a half weeks!

Just give it time.

PS. You might wanna raise your temperatures to the ideal 84-86F range.

Good luck

Pluto


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OfflineKalix
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Registered: 03/20/05
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Re: inoculation [Re: Fizikz]
    #4171182 - 05/13/05 04:12 PM (12 years, 7 months ago)

Quote:

Fizikz said:
Raise the temps a little bit try to get it up to 85, you should be ok if you didnt get the substrate to wet. Remember moist, not wet.




I got full colonization of Hawk's Thai Koh Samui in 7 days and under by keeping my temps @ 86 degrees...


--------------------


My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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Offlinehardwaters
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Registered: 04/11/05
Posts: 137
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Re: inoculation [Re: Fizikz]
    #4171882 - 05/13/05 07:04 PM (12 years, 7 months ago)

I raised the temp to 86 but now I am seeing a yellow/orange liquid in the bottom of the jars. I got these jars in a kit I got on EBAY. The person who sold it to me directed me to this site. I wish I knew about this site before I bought the kit. I still have 2 syringes left should I start over with fresh jars or reinoculate the ones I have going?

Thanks,


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InvisibleRoadkillM
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Re: inoculation [Re: hardwaters]
    #4171901 - 05/13/05 07:11 PM (12 years, 7 months ago)

Quote:

now I am seeing a yellow/orange liquid in the bottom of the jars




sounds like you might have a bacterial contam.

let the jars go for a few more days...and see what happens before tossing them.

---

in the mean time...since you have some extra spore syringes.

go pick up some new jars and supplies...and follow a tek...and make up some new jars.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisibleunknown28
Chill

Registered: 03/24/05
Posts: 258
Loc: Midwest Swanging
Re: inoculation [Re: Roadkill]
    #4171942 - 05/13/05 07:26 PM (12 years, 7 months ago)

Would you guys say 4cc's for a 1 quart size jar is too much??


--------------------
Strains For Trade!!! B+, Hawaiian, Oakridge,Texas


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Offlinescatmanrav
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Registered: 05/08/04
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Re: inoculation [Re: unknown28]
    #4172158 - 05/13/05 08:31 PM (12 years, 7 months ago)

Yes. 1 cc


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleunknown28
Chill

Registered: 03/24/05
Posts: 258
Loc: Midwest Swanging
Re: inoculation [Re: scatmanrav]
    #4175218 - 05/14/05 05:44 PM (12 years, 7 months ago)

That's it?? It seem's like my jars take forever with just 2 cc's


--------------------
Strains For Trade!!! B+, Hawaiian, Oakridge,Texas


Edited by unknown82469 (05/14/05 05:44 PM)


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Amazon Shop: ½ Pint Jars

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