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Amazon Shop for: ½ Pint Jars, Rye Grain

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Offlinecyalume
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Registered: 12/17/04
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Last seen: 10 years, 9 days
26 days.. almost nothing?
    #4167195 - 05/12/05 06:04 PM (11 years, 6 months ago)

26 days ago I inoculated 12 PF-tek jars with GT spores. I'm seeing activity in only several jars.. any idea what might be wrong?

The first maybe 15 days temps were lower around 72, but after i got a new tank heater temps have held at a steady 81F.

TiT setup holding a steady 81F w/towel on top of jars and lid.


pics of the jars with signs of mycellium (3 jars)

same jar as above


Most of the jars look like this:


Edited by cyalume (05/12/05 08:28 PM)


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OfflineSammy
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Registered: 09/03/02
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Re: 26 days.. almost nothing? [Re: cyalume]
    #4167209 - 05/12/05 06:06 PM (11 years, 6 months ago)

Did you shake the syringe before you started the inoculation process??

Sam


--------------------

I believe in the Golden Rule ? The Man with the Gold . . . Rules.
- Mr. T


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Offlinecyalume
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Re: 26 days.. almost nothing? [Re: Sammy]
    #4167269 - 05/12/05 06:16 PM (11 years, 6 months ago)

woops, sure didnt

but i have two syringes of another strain i will go ahead and inoculate the jars with


Edited by cyalume (05/12/05 06:17 PM)


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OfflineSammy
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Re: 26 days.. almost nothing? [Re: cyalume]
    #4167319 - 05/12/05 06:25 PM (11 years, 6 months ago)

Hold on! Check for KARO TEK, or HONEY TEK in the search function. You can multiply the amount of jars you can inoculate with only 1cc of a spore syringe. You'll thank me for it.

Sam


--------------------

I believe in the Golden Rule ? The Man with the Gold . . . Rules.
- Mr. T


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OfflineWronguy
Registered: 03/05/05
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Re: 26 days.. almost nothing? [Re: Sammy]
    #4167335 - 05/12/05 06:29 PM (11 years, 6 months ago)

If you are going to reinoculate those jars I would highly suggest you PC them again. They are no longer sterile. Don't take the chance of losing more jars to contaminates.


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Offlinebrewwhaha1
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Registered: 02/13/05
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Re: 26 days.. almost nothing? [Re: Wronguy]
    #4167406 - 05/12/05 06:49 PM (11 years, 6 months ago)

it could be the spores. I recently have had the same problem. and it is most definatly the spores. rye grain was inoculated from a syringe (z strain) growth was very slow with now growth in many jars. i thought it might be the grain. so i started a karo jar with an old print i had (diff strain.) and uded it to knock up 4 rye jars prepared at the same time the z's were done. the the jars from the karo are now fully colonized the z's are not.

To prove this one step further i had five old syringes amazonian equadors tapalpa (z strain X2) I created 6 karo jars one for each syringe and a controle jar for nothing to grow in. There is growth in all the jars except the controle jar. however both z strains are growing like little hairs, all idividual. The rest are typical mycelium masses seen in karo or honey jars.

the vendor who sold me the z strain did replace the syringes and soon i plan on trying a karo jar for the two replacements (also z strain). hopefully there will be better growth.

However the z strain jars that do have growth i'm unsure of what to do with them. if they are growing that slowly i doubt they will fruit. I could spawn it to manure but i'm afraid its a waist of time and effort.

Sorry this is long winded but people tend to say you have contams and that your temps are wrong etc. etc. this may be true however sometimes its the spores. it was in my case


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Offlinecyalume
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Last seen: 10 years, 9 days
Re: 26 days.. almost nothing? [Re: brewwhaha1]
    #4167764 - 05/12/05 08:26 PM (11 years, 6 months ago)

I contacted the spore supplier who is a shroomery sponsor.. im hoping they will send me two more syringes.


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Amazon Shop for: ½ Pint Jars, Rye Grain

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