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Offlineextinguishment
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thai-koh samui mycelium at day 11
    #4165036 - 05/12/05 01:27 AM (18 years, 10 months ago)

...or about day 15 from spore inoculation. these are pf jars from AFOAF in amsterdam. hopefully i will get to travel there this summer!  :nut: he said one of them has possible contam... there are 2 pictures of that in different hues. they are kept at 28C-29C all the time with a small electric heater with a digital therm. he also said daily they are shaken slowly, vertically and lid facing down, just enough for the cake to move out some of the CO2 buildup. they are on average 60%-75%  colonized after 11 days of mycelial growth. do any of you know if this may be contam?  :cheers:





Edited by extinguishment (05/12/05 01:29 AM)

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Offlinegratefulredhead
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Re: thai-koh samui mycelium at day 11 [Re: extinguishment]
    #4165062 - 05/12/05 01:38 AM (18 years, 10 months ago)

it is often the case that there is a little wetness with mycelium growth... but that is looking a bit suspect... what color is that? green? or blue??

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Offlineextinguishment
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Re: thai-koh samui mycelium at day 11 [Re: gratefulredhead]
    #4165093 - 05/12/05 01:45 AM (18 years, 10 months ago)

kindof a blueish-green, more green.. only 1 jar has experienced this but and it has a few little bb sized specks like this, not concentrated in one area but all over. maybe about 5. i dont think its verm...


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Offlinegratefulredhead
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Re: thai-koh samui mycelium at day 11 [Re: extinguishment]
    #4165107 - 05/12/05 01:48 AM (18 years, 10 months ago)

green is not good. occasionally you see some bluing, if you've shaken with force, but usually that is clearly blue.

the only thing to do now, though, is wait. if it colonizes fully then you'll know. if not, you have other jars!!

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OfflineOl_Mr_Owl
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Re: thai-koh samui mycelium at day 11 [Re: extinguishment]
    #4165112 - 05/12/05 01:48 AM (18 years, 10 months ago)

That looks like a jar Mr. Owl just threw out. Sorry, but that one might need to be tossed.

Good luck.

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OfflineIGnosticAbhorI
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Re: thai-koh samui mycelium at day 11 [Re: Ol_Mr_Owl]
    #4165125 - 05/12/05 01:52 AM (18 years, 10 months ago)

Looks to me like a knot of meanie greenie, i've had it enough :smirk:

Gl

-Gnostic

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Offlineextinguishment
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Re: thai-koh samui mycelium at day 11 [Re: Ol_Mr_Owl]
    #4165169 - 05/12/05 02:02 AM (18 years, 10 months ago)

they were not shaken with force. the spots are on non-colonated substrate.




also in the middle of one island-area of mycelium (in the same jar with the green) it has turned to a slightly not-so-white look.



one thing i just thought of is i remember how he was telling me the shaking method. on that one it look like verm is all over so maybe it was shaken with the lid facing up and with verm tubling down the sides, contam was also introduced. these were all pc'ed 15psi for over an hour. hypothesis: keep jars facing lid-down always and/or never shakes it to try and get more CO2 drainage.  :wtf:

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OfflineOl_Mr_Owl
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Re: thai-koh samui mycelium at day 11 [Re: extinguishment]
    #4165181 - 05/12/05 02:05 AM (18 years, 10 months ago)

There seems to be a lot of empty space in the jar (first picture). Does your friend have dry verm on top? These definitely help to keep the contams out.

Good luck.

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OfflineIGnosticAbhorI
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Re: thai-koh samui mycelium at day 11 [Re: Ol_Mr_Owl]
    #4165195 - 05/12/05 02:12 AM (18 years, 10 months ago)

It's Greenie meanie...

Quote:

it has turned to a slightly not-so-white look.



It Begins as white and then turns green....

Don't worry about co2 until the last week :thumbup:

The greenie started from no-where....And it typically is only on grains, but it obvisouly is on/in your BRF mix, and it needs to be sterilyzed at temps. above the boiling point of water..My point is...

If you get greenie on Brf, then you should PC next time, boiling doesn't kill it...I donno lol, Good luck man

-Gnostic

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Offlineextinguishment
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Re: thai-koh samui mycelium at day 11 [Re: IGnosticAbhorI]
    #4165215 - 05/12/05 02:31 AM (18 years, 10 months ago)

Quote:

extinguishment said:
these were all pc'ed @ 15psi for over an hour.



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OfflineSammy
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Re: thai-koh samui mycelium at day 11 [Re: extinguishment]
    #4165493 - 05/12/05 07:17 AM (18 years, 10 months ago)

When in doubt, toss it out.

Sam


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Offlinelordoftheshroomz
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Re: thai-koh samui mycelium at day 11 [Re: Sammy]
    #4165750 - 05/12/05 09:06 AM (18 years, 10 months ago)

thats mean green so either toss it out or clone some of that mycelium using hydrated corregated cardoard using this tek


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