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InvisibleTien
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Isolating healthy myc
    #4158008 - 05/10/05 04:58 PM (12 years, 3 months ago)

So I got my petri dishes and some PDA.
I have a contaminated Cambodian syringe lying around. It's contammed with lipstick mold.

What I want to do is attempt to grow the mycellium on agar and try to isolate a healthy spot..would this work? Has it been dones successfully? I only have two strains right now and I really don't wanna toss them Cambodians..

Pluto


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Offlinesleepingbigguy
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Re: Isolating healthy myc [Re: Tien]
    #4158310 - 05/10/05 06:17 PM (12 years, 3 months ago)

try this link, scroll dowon just a little bit and there should be some information about the "hot agar tek". ......

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4100718/an/0/page/1

sounds like a smart idea.


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OfflineRogerRabbitM
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Re: Isolating healthy myc [Re: Tien]
    #4158322 - 05/10/05 06:20 PM (12 years, 3 months ago)

Sure. If the lipstick mold starts growing, be sure to use a glovebox and not a flowhood. A flowhood will blow the mold spores everywhere.

I'd place only one drop from the syringe on the center of a petri dish. Immediately transfer any mushroom mycelium you find to a new dish. Make several dishes in advance to transfer into. As soon as the mycelium starts growing in the new dish, transfer some of the new growth to a second new dish before the mold has a chance to get going. I've had it take as many as four transfers to get a clean culture.
RR


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Invisibletahoe
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Re: Isolating healthy myc [Re: RogerRabbit]
    #4158421 - 05/10/05 06:50 PM (12 years, 3 months ago)

since the water in the syringe wont stay in one spot on the dish i would squirt the whole syringe onto a coffee filter and let it dry. Now use the spores on the coffee filter to do your agar work


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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I need to proofread


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InvisibleTien
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Re: Isolating healthy myc [Re: tahoe]
    #4158464 - 05/10/05 07:05 PM (12 years, 3 months ago)

Quote:

tahoe said:
since the water in the syringe wont stay in one spot on the dish i would squirt the whole syringe onto a coffee filter and let it dry. Now use the spores on the coffee filter to do your agar work




....brilliant, that's 5 shroom tip!


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Invisibletahoe
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Re: Isolating healthy myc [Re: Tien]
    #4158565 - 05/10/05 07:26 PM (12 years, 3 months ago)

Np
No problem, not nicca please


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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