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Offlinecloudcity
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newbie needle question
    #4147500 - 05/07/05 05:57 PM (11 years, 10 months ago)

Ive got some jars that are now drying and Ill bee inoculating them later tonight.
I wondering about sterilizing the needle, this will be my first attempt and with somewhat limited resource-

I will be using a bic lighter and plan to wipe the needle after heating with a paper towel and 99% isopropyl

will this be safe?


many thanks


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OfflineGatorB
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Re: newbie needle question [Re: cloudcity]
    #4147507 - 05/07/05 05:58 PM (11 years, 10 months ago)

Yes. Be sure to sterilize after each innoc.


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Offlineshroomguitar420
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Re: newbie needle question [Re: cloudcity]
    #4147508 - 05/07/05 05:58 PM (11 years, 10 months ago)

why are you drying your jars out....?


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InvisibleHolydiver
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Re: newbie needle question [Re: cloudcity]
    #4147524 - 05/07/05 06:00 PM (11 years, 10 months ago)

That's fine, but flame sterilization isn't needed. For needles and inoculation loops, a quick wipe in 70% isopropyl has never let me down. 70% evaporates slower than 99%, so it's a better buy than 99%. Just a note for your future reference.


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To find a place to live between the negatives and positives.


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Offlinecloudcity
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Re: newbie needle question [Re: Holydiver]
    #4147544 - 05/07/05 06:05 PM (11 years, 10 months ago)

drying!!??

I meant cooling..

.

wow thanks for the swift responses

yeah it seems 70% is a better way to go, but I rushed to get 99% before I saw the additions in the sterile tek-




thanks again


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Offlinekratomboy
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Re: newbie needle question [Re: cloudcity]
    #4147812 - 05/07/05 07:40 PM (11 years, 10 months ago)

I flame sterilize between each jar, and wipe with 70% alcohol between each hole.  This might be overkill, but I would rather be safe than sorry.  Good luck  :grin:


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:kodama:"We must see that consciousness is neither an isolated soul nor the mere function of a single nervous system, but of that totality of interrelated stars and galaxies which makes a nervous system possible."


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InvisibleShroomOmatic
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Re: newbie needle question [Re: kratomboy]
    #4147816 - 05/07/05 07:43 PM (11 years, 10 months ago)

70% should do you just fine. Good luck :grin:


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OfflineMighty Bop
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Re: newbie needle question [Re: ShroomOmatic]
    #4147871 - 05/07/05 08:14 PM (11 years, 10 months ago)

Using my portable range, after each jar, I clean the needle with alcohol wipes, then hold over the range for a bit. I don't like the fact that lighters leave the soot on the needle. An alcohol flame would be better.


--------------------
"One attaineth whatever state of being one thinketh about at the last when relinquishing the body, being ever absorbed in the thought thereof." - Sri Krishna to Arjuna, Bhagavad Gita, viii, 6


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InvisiblePrisoner#1M
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Re: newbie needle question [Re: Mighty Bop]
    #4147934 - 05/07/05 08:46 PM (11 years, 10 months ago)

holding the needle in the blue portion or about 3/8 inch above the flame will prevent soot buildup with butane lighters


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