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agar
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Registered: 11/21/04
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:LIQUID CULTURE - THE EASY WAY: 18
#4141612 - 05/06/05 09:52 AM (18 years, 8 months ago) |
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:LIQUID CULTURE - THE EASY WAY:
(how to turn 2ml/cc of spore solution into 20 ??..ten ml/cc syringes - right - cheap & easy.
Assuming you have an empty syringe & another with spore solution.
Go to Kmart, Target or any large discount Mart & buy a 3 pack of EvenFlow plastic baby bottles.
Also buy a small bottle of Karo corn syrup, a tube of silicone sealant & a few glass marbles.
All the baby bottle parts (bottle - screw cap - nipple) will withstand sterilization in a pressure cooker.
Remove the nipple, apply a small amount (1/8th to ? inch) of silicone sealant to the inside (interior) of the nipple, to serve as a self sealing syringe needle injection point. Allow the sealant to cure overnight
Note: the baby bottle has milliliter (ml) graduation marks up to 240 ml (8 fluid ounces).
Add 200 ml of water to the baby bottle (use marks on it - as a measuring point).
Take your empty syringe and aspirate 6 (six) ml of karo syrup into it.
Then inject that into the open baby bottle, resulting in an approximate 3% solution.
Next, add a glass marble to the baby bottle, to serve as an agitator devise - later.
Insert the nipple into the baby bottle (facing down - inside the baby bottle).
Attach the screw ring lid to hold the nipple in place over the mouth of the baby bottle.
:IMPORTANT: Attach the screw ring loose, to allow steam to escape & pressure equalization during pressure cooking.
Pressure cook the baby bottle (or several) 20 minutes @ 15 psi.
:IMPORTANT: Allow the pressure cooker to cool to room temperature, otherwise a vacuum can form inside the bottle, if the screw ring is tightened while the bottle is hot..
Open the cooled pressure cooker & immediately tighten the screw rings on the bottle (or bottles).
You now have a sterilized nutrient solution, in which to inject 2 or 3 ml/cc of spore solution into.
Once you inject a spore solution into it, simply incubate it around 78 to 82F in a clean environment.
You should be able to see tiny strands of mycelium (from germinating spores) in the solution within several days. Be patient, as some spores take longer to germinate - than others.
Once you have visible mycelium in the culture, gently agitate in on a daily basis.
Once the mycelium in the culture is fairly thick, it is ready to be aspirated into sterilized syringes, and used an inoculate.
~~~~~~~
FOR THOSE WITHOUT A PRESSURE COOKER:
NOTE: Not as fool/proof - fail/safe as pressure cooking sterilization.
Cut a piece of tyvek in a circle. Use a rubber band to hold it in place over the screw cap of the baby bottle.
Insert the baby bottle into your microwave (be sure the lid is somewhat LOOSE).
Then, microwave heat it until the solution in it - comes to a boil.
Then - stop, wait a moment & do the same thing again. Repeat, several times.
Remove the baby bottle (gently - as the lid is still loose) & allow to cool to room temperature.
Then, tighten lid.
~~~~~~~~~~~~~~

Or, if you want to do gallons, use jars - with filter lids.
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jtk
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4141640 - 05/06/05 09:59 AM (18 years, 8 months ago) |
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Hey agar nice tek. I just did a karo LC and used 5 ounces of water to about 6 ml karo, or a little over a teaspoon. Is that too much karo?
-------------------- Don't let your brain tell your mind it can't feel
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Sammy
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4141641 - 05/06/05 09:59 AM (18 years, 8 months ago) |
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Hands down.. a GREAT way to do LC!! I'm going to Targyworld today to pick me up some supplies!! Agar... thanks again for your contri. These pics + great explanation make this project easy even for a nEwB
Samuel
-------------------- I believe in the Golden Rule ? The Man with the Gold . . . Rules. - Mr. T
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HippieChick
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4141649 - 05/06/05 10:00 AM (18 years, 8 months ago) |
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Nice Tek. The silicon fills the inside of the nipple, right. You don't need to PC these and filter through two coffee filters and then re PC? Peace,Love,Happiness and Harmony. Hippie Chick
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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Sammy
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Sammy]
#4141651 - 05/06/05 10:00 AM (18 years, 8 months ago) |
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JTK: I used 1 tablespoon of Karo for my 1/2 pint jars of LC.. it's worked great.
Sam
-------------------- I believe in the Golden Rule ? The Man with the Gold . . . Rules. - Mr. T
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agar
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: HippieChick]
#4141684 - 05/06/05 10:07 AM (18 years, 8 months ago) |
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Quote:
HippieChick said: Nice Tek. The silicon fills the inside of the nipple, right. You don't need to PC these and filter through two coffee filters and then re PC? Peace,Love,Happiness and Harmony. Hippie Chick
I could make a LC, just looking at that AVI. (JOKE)
This formula is karo, it doesnt get much gunk in it - when you PC.
Sealing the nipple with silicone simply makes it AIR & WATER TIGHT.
It adds another protective injection port layer, that RESEALS when you pull out the needle.
So, no need to cool, drain, strain & re/PC, as with light dry malt & dry dextrose. Which, I believe is a better nutriant solution. I just did this one with Karo, so the new hands could get an LC togather - QUICK & EASY. It is not optimal, but works well enough to produce plenty of LC myc.
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Lucid_Euphoria
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Sammy]
#4141699 - 05/06/05 10:12 AM (18 years, 8 months ago) |
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Great and simple way to do LC there Agar. I'm gonna give this a try in a month or two on my next grow. I do have a question about LC since I've only read minimally about it. How long can you keep the LC before it becomes dormant/unusable? Also how should it be stored for keeping? I'd assume you don't want to keep it at incubation temps once you've gotten a prolific mycellium culture?
-------------------- PAN CYAN & AZURE FOR TRADE "If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha "Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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HippieChick
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Lucid_Euphoria]
#4141748 - 05/06/05 10:21 AM (18 years, 8 months ago) |
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I've heard they stay good for months if stored in the fridge. They don't need to be warmed up before using. Peace,Love,Happiness and Harmony. Hippie Chick
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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agar
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: HippieChick]
#4141788 - 05/06/05 10:28 AM (18 years, 8 months ago) |
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The lovely LADY heard RIGHT.
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Lucid_Euphoria
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4141810 - 05/06/05 10:33 AM (18 years, 8 months ago) |
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Exccccelent! I'm just finishing my first grow now (about to birth my bulk tubs in about 5 days).
Then I gotta put all my stuff away until we renew our lease in a month and a half so if Mr. Landlord decides to do a once over I don't have to explain all the odd ghettofied science lab shit. After that though I'm definately gonna make a couple LC jars and do a second bulk grow. Gonna try out the Equadors next time around.
Thanks for the info. Catch ya on the flipside!
-------------------- PAN CYAN & AZURE FOR TRADE "If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha "Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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blackout


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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Lucid_Euphoria]
#4141890 - 05/06/05 10:49 AM (18 years, 8 months ago) |
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Nice guide, I like the fact the baby bottle has the graduations already on it. Some may ask why you put a filter on the microwaved bottle, it is because while cooling it will suck air in through the loose threads on the bottle. If I do not use a filter I always make sure to leave it in the microwave overnight without opening it at all so the air it does draw in will be pretty much free from contaminants. Wipe the microwave down with disinfectant before you use it (you should do this regularly anyway). Also instead of stopping and starting the microwave you can leave it full until it starts to boil and then turn it down to defrost for 15-20mins. Remember in the microwave you will be boiling off liquid so you need to add more water and less karo/honey at the start since it will get concentrated more as the water boils off (should stay the same water content in the pc). I would also recommend shaking the bottle well after it has cooled to aerate the mixture with oxygen which has been driven off by boiling. Mycelium will grow better in aerated liquid
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agar
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: blackout]
#4141974 - 05/06/05 11:12 AM (18 years, 8 months ago) |
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--------------------
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Aeon01
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4142126 - 05/06/05 11:54 AM (18 years, 8 months ago) |
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blackout you mentioned using honey as a substitute to karo.. this liquid culture procedure is very interesting and i was wondering what the difference between honey and corn syrup is, both in terms of mycelium development and ratio, and the differences between corn syrup and honey..?
-------------------- "You took too much man.. Too much.. Too much.."
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scatmanrav
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Aeon01]
#4142361 - 05/06/05 01:04 PM (18 years, 8 months ago) |
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How does the baby bottle breathe? Is it unnessesary for gas exchange on LCs?
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blackout


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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Aeon01]
#4142370 - 05/06/05 01:06 PM (18 years, 8 months ago) |
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Ratio is about the same, sugar is not 100% sugar by weight but it is probably best to treat it as such, it is better to have slightly less sugar than slightly more in liquid innoculants. Both will have nutrients for the mycelium to feed on, the honey probably has a bit more but has other things floating in it which make it harder to see what is going on. You can boil up honey and then filter it or decant it off the sediment resulting in a clearer jar so you can clearly see growth (and contams). agar already mentioned the need for straining dry malt extract solutions.
I cannot get karo where I live, most people can get honey. I reckon maple syrup would work too.
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blackout


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Re: :LIQUID CULTURE - THE EASY WAY: [Re: scatmanrav]
#4142378 - 05/06/05 01:08 PM (18 years, 8 months ago) |
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Quote:
scatmanrav said: How does the baby bottle breathe? Is it unnessesary for gas exchange on LCs?
It helps but I dont bother myself and have no problems. you should shake up the jar well to aerate it after boiling (which drives off the dissolved oxygen). The catch 22 with a filter disc is that you cannot shake it very hard for fear of soaking the filter which will invite contams.
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agar
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: scatmanrav]
#4142713 - 05/06/05 02:33 PM (18 years, 8 months ago) |
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Quote:
scatmanrav said: How does the baby bottle breathe? Is it unnessesary for gas exchange on LCs?
As I stated, the method is not optimal. The reason why is lack of gas exchange. To cure that with the BB/LC tek, you simply cut a piece of tyvek in a circle & use rubber bands to hold it around the BB botle. That way.... once in awhile.. you can twist the cap a 1/2 turn loose, to allow for some gas exchange.
Also, once you have healthy myc in a BB/LC, place it in the fridge - to stall it.
I don't use the method. I just wrote the BB/LC tek, for the novices to get a start on LC's.
I use larger glass containers (quarts - half gallon & gallons), with rubber tubing through the lid, silicone sealed down & polyfil stuffed, to allow for gas exchange (as in the pic's below).
I also have a magnetic stir plate & place a stir bar in each container. Then, use the stir plate to agitate the culture, as well as shred it into TINY pieces. So, I don't have the problem of getting the filters wet. If need be, I can also clamp them off.
What is GREAT about the rubber tubing is, you remove the polyfil in one, insert a sterile syringe, tilt the jar, asperate culture out, withdraw the syringe & reinsert the polyfil. No drips, no mess.
Also, with two (2) tubes, when you asperate culture out of one, it pulls air in the other filtered tube. So, you don't create a vacuum in the jar.
With this tubing method & without a stir plate, you simply clamp off the tubing, then shake the hell out of the jar. Then, set the jar upright & remove the clamps.
  
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Edited by agar (05/06/05 02:51 PM)
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GnuBobo
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4142723 - 05/06/05 02:36 PM (18 years, 8 months ago) |
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Agar...I don't understand how the tubes function on the quart jars of LC shown. Could you give a quick description of how/why they're used? Or...so the filter won't get soaked?
-------------------- Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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agar
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo]
#4142770 - 05/06/05 02:48 PM (18 years, 8 months ago) |
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Quote:
GnuBobo said: Agar...I don't understand how the tubes function on the quart jars of LC shown. Could you give a quick description of how/why they're used? Or...so the filter won't get soaked?
We must have been writing - about the same time. See my post right above - your last post.
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GnuBobo
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4142815 - 05/06/05 02:57 PM (18 years, 8 months ago) |
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wicked. Thanks.
-------------------- Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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backupwards
peon

Registered: 04/02/05
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo]
#4143011 - 05/06/05 03:36 PM (18 years, 8 months ago) |
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agar you are the man. i like the bb/lc, but i think i am going with the glass as well because i have no worries about autoclaving the glass. do you think the bb will hold up to the autoclave? i would like to try it and see if there is any difference between the two.
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backupwards
peon

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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4143020 - 05/06/05 03:38 PM (18 years, 8 months ago) |
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blackout like the little pic in your sig, how long did it take you to sit and put that cow together?
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agar
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4143036 - 05/06/05 03:42 PM (18 years, 8 months ago) |
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Baby Bottle, screw ring & nipple are designed to be sterilized by EVENFLO PRODUCTS COMPANY. They will withstand my autoclave & AA 941 Sterilizer. I tested them in both for 30 minutes each at 20 psi.
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backupwards
peon

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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4143056 - 05/06/05 03:46 PM (18 years, 8 months ago) |
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great agar then i think i will give them a try. i do like the flexibility of the nipple for getting the syring in and out easily. must check it out man. any concerns that you might have with using this tek.
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scatmanrav
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4143280 - 05/06/05 04:41 PM (18 years, 8 months ago) |
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This one works too, I guess I'll throw it in here. Not to draw attention from agars post, just another quick simple dealy...
Grab a Rubbermaid Cool Contors bottle (like the left) and pop off the white thing.

You could stuff polyfil in the hole and go, or you can cut/melt/drill a small whole in the side (like 1/4" or smaller or something) and silicone a peice of tyvek over it. Then throw in a self healing injection port in the top. https://www.sciplus.com/lw/category.cfm?category=50
You dont need to seal it with anything, it fits in snugly. The whole bottle (including the white top, even though thats the only part thats not PP5) was made with karo, and PCed at 15-17 PSI for 20 minutes. As you can see, it held up fine. Nice Pan Cyan culture growing in there. Thats 600ml of water in it BTW.
I love them self healing ports.
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agar
old hand


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Re: :LIQUID CULTURE - THE EASY WAY: [Re: scatmanrav]
#4143732 - 05/06/05 06:53 PM (18 years, 8 months ago) |
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Scat....... 
You are not drawing attention away from this. Hell, it's all about LC's.
Do you know that life is just SWEET.
Early Friday evening, weather is warm, a big pitcher of Cadillac Margarita?s, a loaf of cheese -garlic French bread, a tossed garden salad, cold slaw, sweet corn on the cob, garlic - butter - shrimp scampi, meaty pork ribs on the BBQ & a great looking women at your side (wanting you).
It just cannot get better than that. The Club later, some Al Green, Keith Sweat music to dance to??..Time to Party.
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lordoftheshroomz
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4143988 - 05/06/05 08:14 PM (18 years, 8 months ago) |
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damn agar ure livin the life, an excellent shroom cultivator, bbqs, what coud one ask for more?
-------------------- MY Glove Box, Automated Terrarium, and Incubator Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest. THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!
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MrMaddHatter
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: scatmanrav]
#4144315 - 05/06/05 10:05 PM (18 years, 8 months ago) |
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Quote:
scatmanrav said: I love them self healing ports.
Hell yea 
Quote:
agar said: Do you know that life is just SWEET.
Hell yea
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GnuBobo
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: MrMaddHatter]
#4144343 - 05/06/05 10:16 PM (18 years, 8 months ago) |
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Just a question to the more experienced LC people here. Two products that are available:

The silicone is "self-sealing"? It's good up to 400 degrees once cured. So this would be used to put a bead over the injection site?
The Permatex, then, is used for applying things--such as tubing to plastic lids for liquid culture?
Which is best to use?
Cheers.
-------------------- Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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seatrip
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: MrMaddHatter]
#4144414 - 05/06/05 10:44 PM (18 years, 8 months ago) |
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Post deleted by seatripReason for deletion: none
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X24
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4144479 - 05/06/05 11:05 PM (18 years, 8 months ago) |
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so ive got a piece off of a cake and got it growing quite well in a half pint. used some of it to start some succesful inoculations(so far). can i do a LC2LC transfer? upsides? downsides?
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agar
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo]
#4144557 - 05/06/05 11:29 PM (18 years, 8 months ago) |
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I use RTV high temp gasket silicone for both.
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agar
old hand


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Re: :LIQUID CULTURE - THE EASY WAY: [Re: seatrip]
#4144563 - 05/06/05 11:31 PM (18 years, 8 months ago) |
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Quote:
seatrip said: hi agar,what gauge needle is used? would a 20g sharp one work
18 gauge is best all around. But, 20 gauge will work, if the myc isn't so thick, it is stringy.
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GnuBobo
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4144578 - 05/06/05 11:34 PM (18 years, 8 months ago) |
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Agar--thanks for the info. And all your help.
-------------------- Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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VALIS
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar] 1
#4146394 - 05/07/05 12:49 PM (18 years, 8 months ago) |
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When I was searching through liquid culture (LC) techniques a couple months back, I was particularly impressed with SixTango's Easy Liquid Culture Container Tek.
I like the use of baby bottles because of the ease and convenience of the nipple - c'mon, who doesn't love a good nipple?
Anyhow, here's what I did:
 Materials - evenflo 4oz glass baby bottle, filter fiber (i.e. polyfill ), glass marble, tubing, scissors.
 Snip nipple just below the tip.
 Push the tubing through bottom of nipple.
 Cut tubing.
 Insert filter material into tubing.
 Drop the marble in.
 Finished product.
Further information for those who don't already know:
Liquid culture is a method where spores, an agar wedge, or mushroom tissue is injected/inserted/placed into a nutritious solution and incubated, for the purpose of inducing mycelium growth, thereby creating a large amount of inoculum that can be used to inoculate cakes or spawn with. The primary benefits of doing this are as follows:
* Lots and lots ( and lots ) of innoculant can be easily created - 1 10cc multi-spore syringe can be turned into 10 jars of LC, and 100mls of LC can create 10 syringes... pretty cool.
* Using LC to inoculate cakes or spawn can vastly speed up colonization - no lag time waiting for spore germination, in addition to the large amount of inoculation points when injected into substrate.
* The LC can be stored over long periods - throw it in your fridge for months.
Following are the beneficial aspects of using a baby bottle as a container for your LC:
* The nipple serves as a self-healing injection point.
* The nipple can be bent over or squeezed just below the filter tube in order to prevent the LC from saturating the filter when shaking or when drawing up solution.
* Easy and quick to prepare - no need to drill or seal.
* Convenient size, and it's nice to have the ml's/oz's right on the bottle.
Other things, not unique to the baby bottle tek, but useful in any LC container:
* The marble is used as means to better stir/mix the LC, and to break up any mycelium clumps that may occur.
* The filter is for gas exchange.
-------------------- Nature is the Technology of the Divine.
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HippieChick
Chicks can do it too!


Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 12 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4146428 - 05/07/05 12:55 PM (18 years, 8 months ago) |
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Was wondering, could one use colonized WBS to make a LC? Peace,Love,Happiness and Harmony. Hippie Chick
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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VALIS
vast activelivingintelligencesystem


Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 11 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: HippieChick]
#4146466 - 05/07/05 01:00 PM (18 years, 8 months ago) |
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Quote:
HippieChick said: Was wondering, could one use colonized WBS to make a LC?
My guess is Yes - any live/viable shroom material/tissue/organic-matter you throw into the LC will cause mycellium to grow.
It's ok to have to have solid material in your LC.
-------------------- Nature is the Technology of the Divine.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4146557 - 05/07/05 01:13 PM (18 years, 8 months ago) |
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LOL...... In another life... I posted here as 6T. agar=SixTango.
Yup, you can use a colonized seed/grain to start an LC.
Just make sure the transfer is done in a CLEAN way.... with MINIMAL open air exposure............otherwise - contam farm.
--------------------
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VALIS
vast activelivingintelligencesystem


Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 11 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4146622 - 05/07/05 01:24 PM (18 years, 8 months ago) |
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Quote:
agar said: LOL...... In another life... I posted here as 6T. agar=SixTango
You should have mentioned that when I originally made the above post ... guess maybe you must have missed it.
People are funny.
-------------------- Nature is the Technology of the Divine.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4146838 - 05/07/05 02:00 PM (18 years, 8 months ago) |
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I did miss it. Excuse = very hung over today.
 To much FUN last night
--------------------
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captmueller
chemical shaman
Registered: 10/25/04
Posts: 115
Last seen: 16 years, 11 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo]
#4149114 - 05/08/05 01:37 AM (18 years, 8 months ago) |
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i use the ultra black and it seems to make good selfhealing injection ports...
namaste,
captmueller
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 25 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4150951 - 05/08/05 06:37 PM (18 years, 8 months ago) |
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Quote:
VALIS said:
Quote:
agar said: LOL...... In another life... I posted here as 6T. agar=SixTango
You should have mentioned that when I originally made the above post ... guess maybe you must have missed it.
People are funny.
Thats why I was making the comments about 6T not knowing what he was doing Hey who gave this thread a three. Goobers.
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Blue Helix
bold hand


Registered: 02/02/03
Posts: 1,565
Last seen: 6 months, 17 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4151489 - 05/08/05 09:21 PM (18 years, 8 months ago) |
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Quote:
You now have a sterilized nutrient solution, in which to inject 2 or 3 ml/cc of spore solution into.
Why so much spore solution? Using a 1/2cc syringe with a 29-gauge needle, I inoculated agar half pints with spore solution. Each jar received only about 100 microliters (about one drop) of clear spore solution, yet those agar surfaces blew up with mycelium in only 3 days. At the end of one week, most surfaces were 60% covered or more!
The point is that if a single 100 microliter drop of spore solution can colonize an entire half pint agar surface in a few days which can then easily make 200 ml of liquid culture when blended, then why use 100X that amount of spore solution for a small 200ml jar of liquid culture? It's wasteful of the spore solution. I wouldn't use over 1/4 cc of spore solution myself. That should be MORE than enough.
Edited by Blue Helix (05/28/05 12:52 AM)
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@cro
new name


Registered: 12/07/02
Posts: 1,224
Loc: The PNW
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4152465 - 05/09/05 03:02 AM (18 years, 8 months ago) |
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You're 6T? Damn, I still have a bottle of your old liquid nutrient solution.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Blue Helix]
#4152603 - 05/09/05 07:01 AM (18 years, 8 months ago) |
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You dont need to inject 2 or 3 ml of spore solution. Any amount (so long as it has a few viable compatable spores in it) will work. More - simply gets the action going faster, IMHO.
I certainly don't advocate WASTING spore solution. At the same time, I prefer to use the content of a spore laden syringe at one sitting, so I do not have partial syringes laying around.
Once - you master mush/cult & PRINT or SPORE SOLUTION MAKING you generaly have more spores in print or solution form - than a single person (or small group) could EVER USE.
I often use a SPORALATOR to make spore solution:
 
1/2 or 1 gallon wide mouth jar (Sun Tea type) - with spigot removed & replaced with an injection / asperation port - that reseals, installed in the hole in the jar - where the spigot was.
Lid altered with a gas exchange filter & install a clip of some sort to hold a cap suspended with a stainless steel treble hook through the caps stem area - inside the container.
Fill container with 1000 / 1500 / 3000 ml water (depending on size of container) & PC the whole container with water inside it 20 minutes @ 15 psi & allow to cool to room temp.
When ready for use, harvest a large cap (veil about ready to tear), inside a G/B or F/H - swab cap top with h202, remove veil, peirce cap stem area with sterile treble hook, open container & attach cap to clip, which hangs over sterile water & will drop spores directly into it (repeat once or twice or more w/same strain cap).
The result is 1000 / 1500 / 3000 ml of spore solution that can be asperated through the injection / asperation port on the side of the jar via a sterile syringe.
Test first syringe on agar/petri/plate to insure spore solution contains no contaminates & (if not) presto - you now have a capacity to fill 100 / 150 / 300 syringes out of a single spore laden solution container - in aseptic conditions.
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 4 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4152692 - 05/09/05 08:03 AM (18 years, 8 months ago) |
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Agar....you're crazy lol
Killer "Spore-A-Lator" btw 
-Gnostic
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: IGnosticAbhorI]
#4152737 - 05/09/05 08:28 AM (18 years, 8 months ago) |
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Quote:
IGnosticAbhorI said: Agar....you're crazy lol Killer "Spore-A-Lator" btw  -Gnostic
Just a FUN hobby.........
--------------------
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Blue Helix
bold hand


Registered: 02/02/03
Posts: 1,565
Last seen: 6 months, 17 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4154692 - 05/09/05 07:09 PM (18 years, 8 months ago) |
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Quote:
I certainly don't advocate WASTING spore solution. At the same time, I prefer to use the content of a spore laden syringe at one sitting, so I do not have partial syringes laying around.
Ah, but you, Agar, are the one who has shown us the secret of using vacutainers to store spore solution...

That way you don't leave you spore solution lying around at all. You just poke the needle through the rubber lid thingy, draw out what you need (say 1/4th CC), and the rest is kept sterile and perfect in the vacutainer.
Edited by Blue Helix (05/09/05 07:14 PM)
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Silly_Cyben
not-quite-newbie

Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 9 months, 13 hours
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4155749 - 05/10/05 12:17 AM (18 years, 8 months ago) |
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Sounds great. Thanks for sharing this.
How long can these bottles be stored in the refrigerator?
William
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down44
Johnson

Registered: 12/02/04
Posts: 177
Last seen: 1 year, 1 month
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Silly_Cyben]
#4155886 - 05/10/05 12:49 AM (18 years, 8 months ago) |
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great tek
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Silly_Cyben]
#4156485 - 05/10/05 07:33 AM (18 years, 8 months ago) |
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Quote:
browilliam said: How long can these bottles be stored in the refrigerator? William
Once you have healthy culture growth, it is BEST to stall them, by placing in the fridge - where they can/will last several months.
 1 gallon LC on a magnetic stir plate
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ticktock
Seeker


Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 13 years, 10 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4163793 - 05/11/05 08:32 PM (18 years, 8 months ago) |
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My 2 cent additional suggestion; Do everything just as described above. After you see the first development of mycelium, inject a volume of 3% peroxide equal to 1/20 the volume of the liquid culture. Within a minute, the mycelium will head for the surface and float. They have caught tiny bubbles of oxygen and will use it up in the next few days. Repeat when they sink again. There you have aeration without the need for super filtered air. ticktock
--------------------
Don't panic!
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davesj1
Stranger

Registered: 04/11/05
Posts: 260
Last seen: 7 years, 11 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4174564 - 05/14/05 12:45 PM (18 years, 8 months ago) |
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can gerber bottles stand up to pressure cooking?
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RootedGrowth
Grain Apprentice

Registered: 10/30/04
Posts: 225
Last seen: 18 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: davesj1]
#4174626 - 05/14/05 01:07 PM (18 years, 8 months ago) |
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nice tek and a tag
-------------------- Death is a reason to live
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 4 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: davesj1]
#4174639 - 05/14/05 01:12 PM (18 years, 8 months ago) |
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Quote:
davesj1 said: can gerber bottles stand up to pressure cooking?
Read the thread, that's what it's there for, this question has already been anwsered...
-Gnostic
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CLUTCH
Stranger


Registered: 04/11/05
Posts: 879
Last seen: 1 month, 6 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: IGnosticAbhorI]
#4175822 - 05/14/05 06:49 PM (18 years, 8 months ago) |
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Well, actually the tek is using EvenFlow bottles, but yea dave, the Gerber bottles will work just as good.
btw nice find there gnostic (in your avatar) I would love to find that growing in my backyard someday.
Edited by CLUTCH (05/14/05 07:10 PM)
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 23 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4177482 - 05/15/05 08:54 AM (18 years, 8 months ago) |
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Quote:
backupwards said: blackout like the little pic in your sig, how long did it take you to sit and put that cow together?
I stole it from another message board! I did the pathetic attempt of the shroom behind him myself.
I have some LC with just glucose growing at the moment, it is not as active as my honey water but it is clearer to see growth. I may try some regular sugar (sucrose) to see if it works, most say it won't but I have never seen somebody try it.
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Abermelin
Gnome Hat

Registered: 03/22/05
Posts: 394
Loc: In The Mycelial Network
Last seen: 18 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: blackout]
#4177567 - 05/15/05 09:36 AM (18 years, 8 months ago) |
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at what point do we feed this solution to a baby?
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vescudero0
ay ay aaaaay

Registered: 01/28/05 
Posts: 418
Last seen: 17 years, 2 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Abermelin]
#4178030 - 05/15/05 11:30 AM (18 years, 8 months ago) |
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very good info, thanx once again---
-------------------- live & enjooooooooooooooooy
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kake
The answer to1984 is 1776.



Registered: 05/06/99
Posts: 2,782
Loc: The 66th harmonic
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: vescudero0]
#4178780 - 05/15/05 03:01 PM (18 years, 8 months ago) |
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VALIS: how do you keep the polyfill from getting soaked while PCing?
-------------------- The answer to 1984 is 1776.
Edited by kake (05/17/05 10:18 AM)
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yodabuds
Back for more ....

Registered: 06/20/05
Posts: 80
Last seen: 1 year, 9 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: kake]
#4319383 - 06/21/05 12:04 AM (18 years, 7 months ago) |
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So If Im doing my math correct here... In my half gallon jar I should only need to use 2cc's of the syrgine?(.5cc per half pint, two pints in a half gallon) Thanks for the info on the agar.... thats a wonderfull indea and my mother does have a pressure cooker i can borrow.... so i think i will make bottles up with remainder of the syringe..... Also after doing much reading I am going to go with the perlite tek and will use tops of butter containers to put my crumbled cake ontop of the perlite.... Hmmm I think my plan is comming together:) Thanks everyone... I can't wait to get started.... Yoda
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finding_self
Stranger
Registered: 05/15/05
Posts: 489
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bling
Edited by finding_self (04/18/06 08:10 PM)
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Wronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: finding_self]
#4319780 - 06/21/05 06:41 AM (18 years, 7 months ago) |
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I love this tek. Thanks for bringing it to light Agar. I knocked some bottles on 06/15 using this tek and saw mycelium forming on 06/19. That's only a total of 4 days. Obviously it'll be weeks before it's really thick, but I'm on my way.
I used 1/2 CC per bottle. That's it. The rest of the syringe is stored in a vacutainer in the fridge.
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aaaa
Stranger
Registered: 12/05/04
Posts: 41
Last seen: 16 years, 7 months
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: agar]
#4812639 - 10/16/05 08:05 PM (18 years, 3 months ago) |
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Post deleted by ilroyalsilReason for deletion: a
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mskip23
Can It All Be So Simple!


Registered: 06/08/05
Posts: 1,522
Loc: Philly
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: aaaa]
#4812789 - 10/16/05 08:32 PM (18 years, 3 months ago) |
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nice
-------------------- url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif] [/url
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jarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: mskip23]
#4812829 - 10/16/05 08:40 PM (18 years, 3 months ago) |
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Okay so if one fills the nipple with silicon to keep "air and water tight"...does this mean LC's don't need air exchange?
-------------------- P. Cubensis Growth Parameters "All mushrooms are edible, but some only once." -- Croatian Proverb
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jarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: jarroddupont]
#4812941 - 10/16/05 09:01 PM (18 years, 3 months ago) |
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bump
-------------------- P. Cubensis Growth Parameters "All mushrooms are edible, but some only once." -- Croatian Proverb
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: jarroddupont]
#4813056 - 10/16/05 09:22 PM (18 years, 3 months ago) |
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Quote:
jarroddupont said: Okay so if one fills the nipple with silicon to keep "air and water tight"...does this mean LC's don't need air exchange?
I prefer some gas exchange. But, sealed - you will still get myc - just not as thick.
--------------------
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jarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4813061 - 10/16/05 09:22 PM (18 years, 3 months ago) |
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Thanks Agar, YOU'RE THE LC KING
-------------------- P. Cubensis Growth Parameters "All mushrooms are edible, but some only once." -- Croatian Proverb
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: jarroddupont]
#4821417 - 10/18/05 04:11 PM (18 years, 3 months ago) |
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going to get supplies after work tonight, to make me some LC's Awesome Agar-6t!!
i am also going to get supplies to make a new drainage screen for my rinsing.... just like the 6t tek. Kudos!
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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unknown28
Chill

Registered: 03/24/05
Posts: 258
Loc: Midwest Swanging
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Oatman2000]
#4821500 - 10/18/05 04:30 PM (18 years, 3 months ago) |
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Yeah I use this tek alot so I can conserve my spores. I put about 3cc's of Argentina in a bottle and the same amount of Burma from ralphsters in another, and had myc. growth in 2 days
-------------------- Strains For Trade!!! B+, Hawaiian, Oakridge,Texas
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Fospher
Crime FightingMaster Criminal


Registered: 02/09/05
Posts: 2,033
Loc: The Netherlands
Last seen: 12 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4821584 - 10/18/05 04:50 PM (18 years, 3 months ago) |
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If Im using agar (no pun intended) to make the LC and not a syringe, will I maintain a single genome during the incubation? The myco wont reproduce different genome type strands, right?
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lardnar
Pu Pu Platter


Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: ticktock]
#5046738 - 12/11/05 01:40 AM (18 years, 1 month ago) |
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Quote:
ticktock said: My 2 cent additional suggestion; Do everything just as described above. After you see the first development of mycelium, inject a volume of 3% peroxide equal to 1/20 the volume of the liquid culture. Within a minute, the mycelium will head for the surface and float. They have caught tiny bubbles of oxygen and will use it up in the next few days. Repeat when they sink again. There you have aeration without the need for super filtered air. ticktock
Anyone have a reason this wouldn't work?
-------------------- If your soul is sence this life is lost ...
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ke1n
Stranger


Registered: 11/15/05
Posts: 359
Last seen: 14 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: lardnar]
#5197590 - 01/19/06 12:44 AM (18 years, 13 days ago) |
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are the marbles really needed? I cant find thsoe fuckers anywhere.
--------------------
Everything that is posted, including pictures and text, are a result of fictional storytelling using images found online and/or created using the latest graphics software. I am a fictional writer who likes to explore the internet world. ------------------------------------ http://www.adobe.com/
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Shdwstr
FSRCanada


Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 1 month
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: ke1n]
#5197703 - 01/19/06 01:28 AM (18 years, 13 days ago) |
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Quote:
ke1n said: are the marbles really needed? I cant find thsoe fuckers anywhere.
Try Toy stores or Dollar stores... They are everywhere!
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joker_66599
NOOB / SMALLTIME GROWER


Registered: 07/18/05
Posts: 758
Last seen: 3 years, 11 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Shdwstr]
#5232912 - 01/27/06 08:57 PM (18 years, 4 days ago) |
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ok going to do this tomorrow sealed my nipples just now lol say once i have my bb and thick white cloud i guess it's going to be now is it going to be all the water will be white or just some. reading the tek makes it sound like all. and one last thing 1/2 pt jars would u still do 1cc of lcc per jar?
Thanx
--------------------
?¿ ¿? I don't really grow mushrooms I just act like it to be coo. ?¿ ¿?
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Nunbuh_Chrubble
I'm just a kittycat


Registered: 01/23/06
Posts: 3,534
Last seen: 10 years, 1 month
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: joker_66599]
#5233398 - 01/27/06 10:36 PM (18 years, 4 days ago) |
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like LC could get any easier!
I think that all noob teks need to begin with LC instructions!
--------------------
"This day is a lover..." ~Rumi
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: Nunbuh_Chrubble]
#5504225 - 04/11/06 04:07 PM (17 years, 9 months ago) |
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Don't baby bottles have air exchanges in the caps? So that a vaccum isn't formed by the baby sucking one down? Apparently the ones for cattle do. My friend decided to scale your tek up. He bought like a 1.5 gallon COW baby bottle and turned it into an LC.
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: cappa]
#5504272 - 04/11/06 04:16 PM (17 years, 9 months ago) |
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if it's not filtered then contam's will get 'sucked' into the container.
This was meant to be simple and no filter....
--------------------
Atheist Chat
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joker_66599
NOOB / SMALLTIME GROWER


Registered: 07/18/05
Posts: 758
Last seen: 3 years, 11 months
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: Snaggletooth]
#5680915 - 05/27/06 04:16 PM (17 years, 8 months ago) |
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damn did this tek back end of jan and still had them. sucked them 2 weeks ago and still alive. wow lol been sitting in my closet. don't have any more jars to use the LC in and don't wanna trash them. what should i do? 2 1/2 maybe more like 2 baby bottles with tons of LC in them. 1 i even opened and put more karo syrup in it. and still no contam (about a month ago)damn i was lucky. and another or the same one i opened and poured some out on a coir/verm mix whould be tight if it works.
well i am just rambling on now
tek worked great for me
Joker
--------------------
?¿ ¿? I don't really grow mushrooms I just act like it to be coo. ?¿ ¿?
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Atheist
Stranger


Registered: 01/24/06
Posts: 13,705
Loc: USA
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: joker_66599]
#5680931 - 05/27/06 04:25 PM (17 years, 8 months ago) |
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Ya it worked for me but don't LCs need gas exchange? Not sure about that but I don't think my baby bottles have it
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etam
it's a hobby...


Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#5682529 - 05/28/06 03:24 AM (17 years, 8 months ago) |
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I used this procedure yesterday with the evenflow bottles. I realize this tek was written over a year ago, so the manufacturer's design of the BB's may have changed.
The evenflow BB's I purchased also have caps at the bottom. Small amounts of water leak out. One of the bottles had significant leakage losing maybe 50ml of water overnight.
I sealed all the leaks with the silicon sealant.
Do you guys think my LC's are probably contam'd, or am I probably straight?
Thanks guys.
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Brainiac
Rogue Scientist


Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: etam]
#5682539 - 05/28/06 03:47 AM (17 years, 8 months ago) |
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Agaer, Where did you find the blue injection / asperation port,I have found some it a Lab Supplie place for $2.00/(24).Where can I find a batter deal on them.
--------------------
Fair is Fair
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etam
it's a hobby...


Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Brainiac]
#5682546 - 05/28/06 03:59 AM (17 years, 8 months ago) |
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Realized I probably should've posted that in the contam section, so I did.
Sorry about that!
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Brainiac]
#5683385 - 05/28/06 01:07 PM (17 years, 8 months ago) |
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Quote:
Brainiac said: Agaer, Where did you find the blue injection / asperation port,I have found some it a Lab Supplie place for $2.00/(24).Where can I find a batter deal on them.
agar is away for a while, look at my journal... the blue injection ports can be found at the website posted, cheepest on the net, but min order of $10.
Just a FYI, the blue one's aren't that good. with a 18 ga. needle it will put a hole in the port, over time it gets pretty worn out. I still use em tho, i got alot.
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Birthbytongue
Apprentice

Registered: 09/14/04
Posts: 206
Last seen: 3 months, 12 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: kake]
#5683490 - 05/28/06 02:14 PM (17 years, 8 months ago) |
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Quote:
kake said: VALIS: how do you keep the polyfill from getting soaked while PCing?
great thread that every newbie should read. Thanks to agar once again. However, i've got this same question as well. When PCing won't your polyfill in the tubing get soaked?
-------------------- -------------------------------------- Proliferation of knowledge is our ONLY weapon!!!!!! WAKE UP the SLEEPING!
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Birthbytongue]
#5910896 - 07/29/06 01:13 AM (17 years, 6 months ago) |
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I love this tek
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stormhalter
full metalalchemist

Registered: 07/10/06
Posts: 175
Loc: Behind you.
Last seen: 16 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Blutjager]
#5911277 - 07/29/06 06:41 AM (17 years, 6 months ago) |
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Wow, fantastic LC Tek.
Very well written and ingenious! I will try this next week. (:
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stormhalter
full metalalchemist

Registered: 07/10/06
Posts: 175
Loc: Behind you.
Last seen: 16 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: stormhalter]
#5911283 - 07/29/06 06:48 AM (17 years, 6 months ago) |
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I had one question. When you fill a sterile syringe with LC, what do you do with what's left over in the jar? Continue to incubate it until it's used up? Or is it thrown in the fridge to stall the solution for a few months?
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noleshroomer
Stranger
Registered: 07/10/06
Posts: 22
Last seen: 17 years, 5 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: stormhalter]
#5931439 - 08/04/06 01:13 PM (17 years, 5 months ago) |
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I followed this tek to a T and its been 4 days now and i dont see much of anything.. is this normal? How long will it normally take to see some mycellium. Im almost worried the syringe was a fake.
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Bamaman
...has issues.

Registered: 08/04/05
Posts: 657
Loc: Down the rabbit hole...
Last seen: 8 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#6095186 - 09/24/06 08:49 AM (17 years, 4 months ago) |
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Hey Agar, just wondering how long it takes for the baby bottles to be ready for use?
-------------------- Diabetes causes hamsters.
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Bamaman
...has issues.

Registered: 08/04/05
Posts: 657
Loc: Down the rabbit hole...
Last seen: 8 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Bamaman]
#6098635 - 09/25/06 01:44 PM (17 years, 4 months ago) |
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Bump
-------------------- Diabetes causes hamsters.
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fufungi
Stranger



Registered: 03/15/05
Posts: 224
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Bamaman]
#6101173 - 09/26/06 12:01 AM (17 years, 4 months ago) |
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I'm glad someone dug up this post. This is the only way i've ever done lc and it has worked great for me. To answer your question I usually continue incubation about a week after the first signs of growth. So about 10 days total.
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Dihnekis
Stranger

Registered: 07/11/06
Posts: 906
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: fufungi]
#6101260 - 09/26/06 12:31 AM (17 years, 4 months ago) |
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How long does it usually take to see growth?
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Dihnekis]
#6101565 - 09/26/06 02:55 AM (17 years, 4 months ago) |
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^^^^from spores: about 8-10 days until fulll maturity. but.... there are much easier ways.
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Lun4e
Stranger


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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#6464653 - 01/16/07 01:35 AM (17 years, 16 days ago) |
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Tek looks great.
Thanks Agar!!!
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etam
it's a hobby...


Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Lun4e]
#6464700 - 01/16/07 02:05 AM (17 years, 16 days ago) |
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I've always had problems with the baby bottles leaking. Never have been able to get this to work for me.
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Primate
former addict


Registered: 08/12/99
Posts: 855
Last seen: 14 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: etam]
#6465106 - 01/16/07 08:11 AM (17 years, 15 days ago) |
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Quote:
etam said: I've always had problems with the baby bottles leaking. Never have been able to get this to work for me.
If you get one of the baby bottles without the little holes on the base of the nipple it won't leak. Alot of the more expensive bottles come with tiny holes poked in the nipple where it fits over the bottle top. You can silicone over these holes but if your not careful, the silicone can make the bottle not give a good seal when you tighten the lid. The bottles at my local dollar store have worked wonderfully for me. Also, drawing the solution from the bottle can be a bit tricky.
All and all though, i havent had any major problems using a baby bottle. Even without any gas exchange.
-Prim
-------------------- "Power to the peaceful" -Micheal Franti and Spearhead
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: ke1n]
#6465114 - 01/16/07 08:15 AM (17 years, 15 days ago) |
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Quote:
ke1n said: are the marbles really needed? I cant find thsoe fuckers anywhere.
2 sharp shards of broken glass has always worked for me
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misterdogman
Educationalresearcher ofthe Shroom



Registered: 03/17/08
Posts: 644
Loc: International Waters in a...
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: yodabuds]
#8179462 - 03/22/08 11:30 AM (15 years, 10 months ago) |
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Theres how many pints in a half gallon? Id recalibrate first but your not here to see that neway I guess...
-------------------- While driving my overpowered car around tight corners like a maniac all my friends yell out. Hey man slow down, we might Wreck. I reply, Shut up, I drive like Dale Earnhardt. They all look around at each other and laugh. Then one asks me. Hey wait, isnt he dead? Well yeah he is I said. Then I drive like Junior I mumble, while I promptly slow down. "Everyday is opposite day. Everything I say is opposite of what you think I am saying. So if I say something and you think I mean one thing it is the opposite of what I am really trying to say. I actually mean another thing other than what you are thinking I am saying. Get it? Good because that's what I meant". "You may defeat me, you may even destroy me, But you will never CONQUER me"!
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Atheist
Stranger


Registered: 01/24/06
Posts: 13,705
Loc: USA
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: misterdogman] 1
#8179488 - 03/22/08 11:40 AM (15 years, 10 months ago) |
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8 pints = 1 gallon
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misterdogman
Educationalresearcher ofthe Shroom



Registered: 03/17/08
Posts: 644
Loc: International Waters in a...
Last seen: 10 years, 9 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Atheist] 1
#8180568 - 03/22/08 05:35 PM (15 years, 10 months ago) |
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yeah I was trying to educate yodabuds who aint here neway, he said there was 2 pints in a half Gallon...lol. Well if thats the case I own the Gas station on the corner, want to buy some Gas? LOL.
-------------------- While driving my overpowered car around tight corners like a maniac all my friends yell out. Hey man slow down, we might Wreck. I reply, Shut up, I drive like Dale Earnhardt. They all look around at each other and laugh. Then one asks me. Hey wait, isnt he dead? Well yeah he is I said. Then I drive like Junior I mumble, while I promptly slow down. "Everyday is opposite day. Everything I say is opposite of what you think I am saying. So if I say something and you think I mean one thing it is the opposite of what I am really trying to say. I actually mean another thing other than what you are thinking I am saying. Get it? Good because that's what I meant". "You may defeat me, you may even destroy me, But you will never CONQUER me"!
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ScavengerType



Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 10 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo] 1
#8180694 - 03/22/08 06:16 PM (15 years, 10 months ago) |
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interesting. My LC method isn't spectacular so perhaps this is a good idea for me.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?" "The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything." - Author and former M6/M5 agent John le Carré on Democracy Now. Conquer's Club
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OwMyHead
Village eediyot



Registered: 04/11/08
Posts: 196
Last seen: 14 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Primate] 1
#8712987 - 08/01/08 04:06 PM (15 years, 5 months ago) |
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I'm so glad I read all this thread, because I do, in fact, have bottles with tiny little holes at the base of the nipple (not at the protrusion, but the lip that sits under the cap when you screw it on.
Perfect solution: a teeny little drip of silicone, and a q-tip. No muss, no fuss, no mess, and it doesn't keep the bottle from sealing because you have too much gunk on the lip.
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 18 hours
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Re: :LIQUID CULTURE - THE EASY WAY: (moved) [Re: agar] 1
#9171545 - 11/02/08 08:29 AM (15 years, 2 months ago) |
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This thread was moved from Mushroom Cultivation.
Reason: -> archive
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