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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Atheist
Stranger


Registered: 01/24/06
Posts: 13,705
Loc: USA
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: joker_66599]
#5680931 - 05/27/06 04:25 PM (17 years, 8 months ago) |
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Ya it worked for me but don't LCs need gas exchange? Not sure about that but I don't think my baby bottles have it
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etam
it's a hobby...


Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#5682529 - 05/28/06 03:24 AM (17 years, 8 months ago) |
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I used this procedure yesterday with the evenflow bottles. I realize this tek was written over a year ago, so the manufacturer's design of the BB's may have changed.
The evenflow BB's I purchased also have caps at the bottom. Small amounts of water leak out. One of the bottles had significant leakage losing maybe 50ml of water overnight.
I sealed all the leaks with the silicon sealant.
Do you guys think my LC's are probably contam'd, or am I probably straight?
Thanks guys.
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Brainiac
Rogue Scientist


Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: etam]
#5682539 - 05/28/06 03:47 AM (17 years, 8 months ago) |
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Agaer, Where did you find the blue injection / asperation port,I have found some it a Lab Supplie place for $2.00/(24).Where can I find a batter deal on them.
--------------------
Fair is Fair
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etam
it's a hobby...


Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Brainiac]
#5682546 - 05/28/06 03:59 AM (17 years, 8 months ago) |
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Realized I probably should've posted that in the contam section, so I did.
Sorry about that!
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Brainiac]
#5683385 - 05/28/06 01:07 PM (17 years, 8 months ago) |
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Quote:
Brainiac said: Agaer, Where did you find the blue injection / asperation port,I have found some it a Lab Supplie place for $2.00/(24).Where can I find a batter deal on them.
agar is away for a while, look at my journal... the blue injection ports can be found at the website posted, cheepest on the net, but min order of $10.
Just a FYI, the blue one's aren't that good. with a 18 ga. needle it will put a hole in the port, over time it gets pretty worn out. I still use em tho, i got alot.
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Birthbytongue
Apprentice

Registered: 09/14/04
Posts: 206
Last seen: 3 months, 12 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: kake]
#5683490 - 05/28/06 02:14 PM (17 years, 8 months ago) |
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Quote:
kake said: VALIS: how do you keep the polyfill from getting soaked while PCing?
great thread that every newbie should read. Thanks to agar once again. However, i've got this same question as well. When PCing won't your polyfill in the tubing get soaked?
-------------------- -------------------------------------- Proliferation of knowledge is our ONLY weapon!!!!!! WAKE UP the SLEEPING!
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Birthbytongue]
#5910896 - 07/29/06 01:13 AM (17 years, 6 months ago) |
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I love this tek
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stormhalter
full metalalchemist

Registered: 07/10/06
Posts: 175
Loc: Behind you.
Last seen: 16 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Blutjager]
#5911277 - 07/29/06 06:41 AM (17 years, 6 months ago) |
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Wow, fantastic LC Tek.
Very well written and ingenious! I will try this next week. (:
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stormhalter
full metalalchemist

Registered: 07/10/06
Posts: 175
Loc: Behind you.
Last seen: 16 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: stormhalter]
#5911283 - 07/29/06 06:48 AM (17 years, 6 months ago) |
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I had one question. When you fill a sterile syringe with LC, what do you do with what's left over in the jar? Continue to incubate it until it's used up? Or is it thrown in the fridge to stall the solution for a few months?
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noleshroomer
Stranger
Registered: 07/10/06
Posts: 22
Last seen: 17 years, 5 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: stormhalter]
#5931439 - 08/04/06 01:13 PM (17 years, 5 months ago) |
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I followed this tek to a T and its been 4 days now and i dont see much of anything.. is this normal? How long will it normally take to see some mycellium. Im almost worried the syringe was a fake.
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Bamaman
...has issues.

Registered: 08/04/05
Posts: 657
Loc: Down the rabbit hole...
Last seen: 8 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#6095186 - 09/24/06 08:49 AM (17 years, 4 months ago) |
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Hey Agar, just wondering how long it takes for the baby bottles to be ready for use?
-------------------- Diabetes causes hamsters.
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Bamaman
...has issues.

Registered: 08/04/05
Posts: 657
Loc: Down the rabbit hole...
Last seen: 8 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Bamaman]
#6098635 - 09/25/06 01:44 PM (17 years, 4 months ago) |
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Bump
-------------------- Diabetes causes hamsters.
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fufungi
Stranger



Registered: 03/15/05
Posts: 224
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Bamaman]
#6101173 - 09/26/06 12:01 AM (17 years, 4 months ago) |
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I'm glad someone dug up this post. This is the only way i've ever done lc and it has worked great for me. To answer your question I usually continue incubation about a week after the first signs of growth. So about 10 days total.
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Dihnekis
Stranger

Registered: 07/11/06
Posts: 906
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: fufungi]
#6101260 - 09/26/06 12:31 AM (17 years, 4 months ago) |
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How long does it usually take to see growth?
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Dihnekis]
#6101565 - 09/26/06 02:55 AM (17 years, 4 months ago) |
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^^^^from spores: about 8-10 days until fulll maturity. but.... there are much easier ways.
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Lun4e
Stranger


Registered: 12/05/04
Posts: 213
Last seen: 16 years, 16 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#6464653 - 01/16/07 01:35 AM (17 years, 16 days ago) |
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Tek looks great.
Thanks Agar!!!
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etam
it's a hobby...


Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Lun4e]
#6464700 - 01/16/07 02:05 AM (17 years, 16 days ago) |
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I've always had problems with the baby bottles leaking. Never have been able to get this to work for me.
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Primate
former addict


Registered: 08/12/99
Posts: 855
Last seen: 14 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: etam]
#6465106 - 01/16/07 08:11 AM (17 years, 15 days ago) |
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Quote:
etam said: I've always had problems with the baby bottles leaking. Never have been able to get this to work for me.
If you get one of the baby bottles without the little holes on the base of the nipple it won't leak. Alot of the more expensive bottles come with tiny holes poked in the nipple where it fits over the bottle top. You can silicone over these holes but if your not careful, the silicone can make the bottle not give a good seal when you tighten the lid. The bottles at my local dollar store have worked wonderfully for me. Also, drawing the solution from the bottle can be a bit tricky.
All and all though, i havent had any major problems using a baby bottle. Even without any gas exchange.
-Prim
-------------------- "Power to the peaceful" -Micheal Franti and Spearhead
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: ke1n]
#6465114 - 01/16/07 08:15 AM (17 years, 15 days ago) |
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Quote:
ke1n said: are the marbles really needed? I cant find thsoe fuckers anywhere.
2 sharp shards of broken glass has always worked for me
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misterdogman
Educationalresearcher ofthe Shroom



Registered: 03/17/08
Posts: 644
Loc: International Waters in a...
Last seen: 10 years, 9 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: yodabuds]
#8179462 - 03/22/08 11:30 AM (15 years, 10 months ago) |
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Theres how many pints in a half gallon? Id recalibrate first but your not here to see that neway I guess...
-------------------- While driving my overpowered car around tight corners like a maniac all my friends yell out. Hey man slow down, we might Wreck. I reply, Shut up, I drive like Dale Earnhardt. They all look around at each other and laugh. Then one asks me. Hey wait, isnt he dead? Well yeah he is I said. Then I drive like Junior I mumble, while I promptly slow down. "Everyday is opposite day. Everything I say is opposite of what you think I am saying. So if I say something and you think I mean one thing it is the opposite of what I am really trying to say. I actually mean another thing other than what you are thinking I am saying. Get it? Good because that's what I meant". "You may defeat me, you may even destroy me, But you will never CONQUER me"!
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