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Offlinecaptmueller
chemical shaman
Registered: 10/25/04
Posts: 115
Last seen: 16 years, 11 months
Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo]
    #4149114 - 05/08/05 01:37 AM (18 years, 8 months ago)

i use the ultra black and it seems to make good selfhealing injection ports...


namaste,

captmueller


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 25 days
Trusted Cultivator
Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
    #4150951 - 05/08/05 06:37 PM (18 years, 8 months ago)

Quote:

VALIS said:
Quote:

agar said:
LOL...... In another life... I posted here as 6T. agar=SixTango




You should have mentioned that when I originally made the above post ... guess maybe you must have missed it.

People are funny.




Thats why I was making the comments about 6T not knowing what he was doing :smile: Hey who gave this thread a three. Goobers.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineBlue Helix
bold hand
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Registered: 02/02/03
Posts: 1,565
Last seen: 6 months, 17 days
Trusted Cultivator
OG Cultivator
Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
    #4151489 - 05/08/05 09:21 PM (18 years, 8 months ago)

Quote:

You now have a sterilized nutrient solution, in which to inject 2 or 3 ml/cc of spore solution into.




Why so much spore solution? Using a 1/2cc syringe with a 29-gauge needle, I inoculated agar half pints with spore solution. Each jar received only about 100 microliters (about one drop) of clear spore solution, yet those agar surfaces blew up with mycelium in only 3 days. At the end of one week, most surfaces were 60% covered or more!

The point is that if a single 100 microliter drop of spore solution can colonize an entire half pint agar surface in a few days which can then easily make 200 ml of liquid culture when blended, then why use 100X that amount of spore solution for a small 200ml jar of liquid culture? It's wasteful of the spore solution. I wouldn't use over 1/4 cc of spore solution myself. That should be MORE than enough.


Edited by Blue Helix (05/28/05 12:52 AM)


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Invisible@cro
new name
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Registered: 12/07/02
Posts: 1,224
Loc: The PNW
Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
    #4152465 - 05/09/05 03:02 AM (18 years, 8 months ago)

:wtf:  You're 6T?
Damn, I still have a bottle of your old liquid nutrient solution.  :lol:


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Blue Helix]
    #4152603 - 05/09/05 07:01 AM (18 years, 8 months ago)

You dont need to inject 2 or 3 ml of spore solution. Any amount (so long as it has a few viable compatable spores in it) will work. More - simply gets the action going faster, IMHO.

I certainly don't advocate WASTING spore solution. At the same time, I prefer to use the content of a spore laden syringe at one sitting, so I do not have partial syringes laying around.

Once - you master mush/cult & PRINT or SPORE SOLUTION MAKING you generaly have more spores in print or solution form - than a single person (or small group) could EVER USE.

I often use a SPORALATOR to make spore solution:



1/2 or 1 gallon wide mouth jar (Sun Tea type) - with spigot removed & replaced with an injection / asperation port - that reseals, installed in the hole in the jar - where the spigot was.

Lid altered with a gas exchange filter & install a clip of some sort to hold a cap suspended with a stainless steel treble hook through the caps stem area - inside the container.

Fill container with 1000 / 1500 / 3000 ml water (depending on size of container) & PC the whole container with water inside it 20 minutes @ 15 psi &  allow to cool to room temp.

When ready for use, harvest a large cap (veil about ready to tear), inside a G/B or F/H - swab cap top with h202, remove veil, peirce cap stem area with sterile treble hook, open container & attach cap to clip, which hangs over sterile water & will drop spores directly into it (repeat once or twice or more w/same strain cap).

The result is 1000 / 1500 / 3000 ml of spore solution that can be asperated through the injection / asperation port on the side of the jar via a sterile syringe.

Test first syringe on agar/petri/plate to insure spore solution contains no contaminates & (if not) presto - you now have a capacity to fill 100 / 150 / 300  syringes out of a single spore laden solution container - in aseptic conditions.  :thumbup:


--------------------


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OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 4 days
Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
    #4152692 - 05/09/05 08:03 AM (18 years, 8 months ago)

Agar....you're crazy lol

Killer "Spore-A-Lator" btw :thumbup:

-Gnostic


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: IGnosticAbhorI]
    #4152737 - 05/09/05 08:28 AM (18 years, 8 months ago)

Quote:

IGnosticAbhorI said:
Agar....you're crazy lol Killer "Spore-A-Lator" btw :thumbup:
-Gnostic




Just a FUN hobby......... :wink: :tongue2: :stoned:


--------------------


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OfflineBlue Helix
bold hand
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Registered: 02/02/03
Posts: 1,565
Last seen: 6 months, 17 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
    #4154692 - 05/09/05 07:09 PM (18 years, 8 months ago)

Quote:

I certainly don't advocate WASTING spore solution. At the same time, I prefer to use the content of a spore laden syringe at one sitting, so I do not have partial syringes laying around.




Ah, but you, Agar, are the one who has shown us the secret of using vacutainers to store spore solution...



That way you don't leave you spore solution lying around at all. You just poke the needle through the rubber lid thingy, draw out what you need (say 1/4th CC), and the rest is kept sterile and perfect in the vacutainer.


Edited by Blue Helix (05/09/05 07:14 PM)


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OfflineSilly_Cyben
not-quite-newbie

Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 9 months, 13 hours
Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
    #4155749 - 05/10/05 12:17 AM (18 years, 8 months ago)

Sounds great. Thanks for sharing this.

How long can these bottles be stored in the refrigerator?

William


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Offlinedown44
Johnson

Registered: 12/02/04
Posts: 177
Last seen: 1 year, 1 month
Re: :LIQUID CULTURE - THE EASY WAY: [Re: Silly_Cyben]
    #4155886 - 05/10/05 12:49 AM (18 years, 8 months ago)

great tek


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Silly_Cyben]
    #4156485 - 05/10/05 07:33 AM (18 years, 8 months ago)

Quote:

browilliam said: How long can these bottles be stored in the refrigerator? William




Once you have healthy culture growth, it is BEST to stall them, by placing in the fridge - where they can/will last several months.


1 gallon LC on a magnetic stir plate


--------------------


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Offlineticktock
Seeker
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Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 13 years, 10 months
Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
    #4163793 - 05/11/05 08:32 PM (18 years, 8 months ago)

My 2 cent additional suggestion;

Do everything just as described above. After you see the first development of mycelium, inject a volume of 3% peroxide equal to 1/20 the volume of the liquid culture. Within a minute, the mycelium will head for the surface and float. They have caught tiny bubbles of oxygen and will use it up in the next few days. Repeat when they sink again.

There you have aeration without the need for super filtered air.

ticktock


--------------------
Don't panic!


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Offlinedavesj1
Stranger

Registered: 04/11/05
Posts: 260
Last seen: 7 years, 11 months
Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
    #4174564 - 05/14/05 12:45 PM (18 years, 8 months ago)

can gerber bottles stand up to pressure cooking?


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OfflineRootedGrowth
Grain Apprentice
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Registered: 10/30/04
Posts: 225
Last seen: 18 years, 3 months
Re: :LIQUID CULTURE - THE EASY WAY: [Re: davesj1]
    #4174626 - 05/14/05 01:07 PM (18 years, 8 months ago)

nice tek and a tag  :smile:


--------------------
Death is a reason to live


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OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 8 months, 4 days
Re: :LIQUID CULTURE - THE EASY WAY: [Re: davesj1]
    #4174639 - 05/14/05 01:12 PM (18 years, 8 months ago)

Quote:

davesj1 said:
can gerber bottles stand up to pressure cooking?




Read the thread, that's what it's there for, this question has already been anwsered...

-Gnostic


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OfflineCLUTCH
Stranger
Male User Gallery

Registered: 04/11/05
Posts: 879
Last seen: 1 month, 6 days
Re: :LIQUID CULTURE - THE EASY WAY: [Re: IGnosticAbhorI]
    #4175822 - 05/14/05 06:49 PM (18 years, 8 months ago)

Well, actually the tek is using EvenFlow bottles, but yea dave, the Gerber bottles will work just as good.

btw nice find there gnostic (in your avatar)  I would love to find that growing in my backyard someday. :shroomer:


Edited by CLUTCH (05/14/05 07:10 PM)


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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 23 days
Trusted Cultivator
Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
    #4177482 - 05/15/05 08:54 AM (18 years, 8 months ago)

Quote:

backupwards said:
blackout like the little pic in your sig, how long did it take you to sit and put that cow together?



I stole it from another message board! I did the pathetic attempt of the shroom behind him myself.

I have some LC with just glucose growing at the moment, it is not as active as my honey water but it is clearer to see growth. I may try some regular sugar (sucrose) to see if it works, most say it won't but I have never seen somebody try it.


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OfflineAbermelin
Gnome Hat

Registered: 03/22/05
Posts: 394
Loc: In The Mycelial Network
Last seen: 18 years, 8 months
Re: :LIQUID CULTURE - THE EASY WAY: [Re: blackout]
    #4177567 - 05/15/05 09:36 AM (18 years, 8 months ago)

at what point do we feed this solution to a baby?


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Offlinevescudero0
ay ay aaaaay

Registered: 01/28/05 Happy 19th Shroomiversary!
Posts: 418
Last seen: 17 years, 2 months
Re: :LIQUID CULTURE - THE EASY WAY: [Re: Abermelin]
    #4178030 - 05/15/05 11:30 AM (18 years, 8 months ago)

very good info, thanx once again---


--------------------
live & enjooooooooooooooooy



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Invisiblekake
The answer to1984 is 1776.
Male User Gallery

Folding@home Statistics
Registered: 05/06/99
Posts: 2,782
Loc: The 66th harmonic
Re: :LIQUID CULTURE - THE EASY WAY: [Re: vescudero0]
    #4178780 - 05/15/05 03:01 PM (18 years, 8 months ago)

VALIS: how do you keep the polyfill from getting soaked while PCing?


--------------------
The answer to 1984 is 1776.


Edited by kake (05/17/05 10:18 AM)


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