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peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo]
#4143011 - 05/06/05 03:36 PM (18 years, 8 months ago) |
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agar you are the man. i like the bb/lc, but i think i am going with the glass as well because i have no worries about autoclaving the glass. do you think the bb will hold up to the autoclave? i would like to try it and see if there is any difference between the two.
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backupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4143020 - 05/06/05 03:38 PM (18 years, 8 months ago) |
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blackout like the little pic in your sig, how long did it take you to sit and put that cow together?
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4143036 - 05/06/05 03:42 PM (18 years, 8 months ago) |
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Baby Bottle, screw ring & nipple are designed to be sterilized by EVENFLO PRODUCTS COMPANY. They will withstand my autoclave & AA 941 Sterilizer. I tested them in both for 30 minutes each at 20 psi.
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peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4143056 - 05/06/05 03:46 PM (18 years, 8 months ago) |
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great agar then i think i will give them a try. i do like the flexibility of the nipple for getting the syring in and out easily. must check it out man. any concerns that you might have with using this tek.
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 25 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4143280 - 05/06/05 04:41 PM (18 years, 8 months ago) |
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This one works too, I guess I'll throw it in here. Not to draw attention from agars post, just another quick simple dealy...
Grab a Rubbermaid Cool Contors bottle (like the left) and pop off the white thing.

You could stuff polyfil in the hole and go, or you can cut/melt/drill a small whole in the side (like 1/4" or smaller or something) and silicone a peice of tyvek over it. Then throw in a self healing injection port in the top. https://www.sciplus.com/lw/category.cfm?category=50
You dont need to seal it with anything, it fits in snugly. The whole bottle (including the white top, even though thats the only part thats not PP5) was made with karo, and PCed at 15-17 PSI for 20 minutes. As you can see, it held up fine. Nice Pan Cyan culture growing in there. Thats 600ml of water in it BTW.
I love them self healing ports.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: scatmanrav]
#4143732 - 05/06/05 06:53 PM (18 years, 8 months ago) |
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Scat....... 
You are not drawing attention away from this. Hell, it's all about LC's.
Do you know that life is just SWEET.
Early Friday evening, weather is warm, a big pitcher of Cadillac Margarita?s, a loaf of cheese -garlic French bread, a tossed garden salad, cold slaw, sweet corn on the cob, garlic - butter - shrimp scampi, meaty pork ribs on the BBQ & a great looking women at your side (wanting you).
It just cannot get better than that. The Club later, some Al Green, Keith Sweat music to dance to??..Time to Party.
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lordoftheshroomz
recoveringaddict


Registered: 01/23/05
Posts: 1,631
Loc: wouldn't you like to kno ...
Last seen: 16 years, 9 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4143988 - 05/06/05 08:14 PM (18 years, 8 months ago) |
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damn agar ure livin the life, an excellent shroom cultivator, bbqs, what coud one ask for more?
-------------------- MY Glove Box, Automated Terrarium, and Incubator Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest. THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!
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MrMaddHatter
Dementia praecox


Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: scatmanrav]
#4144315 - 05/06/05 10:05 PM (18 years, 8 months ago) |
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Quote:
scatmanrav said: I love them self healing ports.
Hell yea 
Quote:
agar said: Do you know that life is just SWEET.
Hell yea
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GnuBobo
Frilly Cuffs Extraordinaire


Registered: 06/17/04
Posts: 43,754
Loc: Charisma
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: MrMaddHatter]
#4144343 - 05/06/05 10:16 PM (18 years, 8 months ago) |
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Just a question to the more experienced LC people here. Two products that are available:

The silicone is "self-sealing"? It's good up to 400 degrees once cured. So this would be used to put a bead over the injection site?
The Permatex, then, is used for applying things--such as tubing to plastic lids for liquid culture?
Which is best to use?
Cheers.
-------------------- Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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seatrip
Stranger


Registered: 09/01/04
Posts: 97
Last seen: 8 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: *DELETED* [Re: MrMaddHatter]
#4144414 - 05/06/05 10:44 PM (18 years, 8 months ago) |
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Post deleted by seatripReason for deletion: none
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X24
pornstar


Registered: 10/01/04
Posts: 378
Last seen: 16 years, 3 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4144479 - 05/06/05 11:05 PM (18 years, 8 months ago) |
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so ive got a piece off of a cake and got it growing quite well in a half pint. used some of it to start some succesful inoculations(so far). can i do a LC2LC transfer? upsides? downsides?
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: GnuBobo]
#4144557 - 05/06/05 11:29 PM (18 years, 8 months ago) |
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I use RTV high temp gasket silicone for both.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: seatrip]
#4144563 - 05/06/05 11:31 PM (18 years, 8 months ago) |
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Quote:
seatrip said: hi agar,what gauge needle is used? would a 20g sharp one work
18 gauge is best all around. But, 20 gauge will work, if the myc isn't so thick, it is stringy.
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GnuBobo
Frilly Cuffs Extraordinaire


Registered: 06/17/04
Posts: 43,754
Loc: Charisma
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4144578 - 05/06/05 11:34 PM (18 years, 8 months ago) |
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Agar--thanks for the info. And all your help.
-------------------- Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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VALIS
vast activelivingintelligencesystem


Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 11 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar] 1
#4146394 - 05/07/05 12:49 PM (18 years, 8 months ago) |
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When I was searching through liquid culture (LC) techniques a couple months back, I was particularly impressed with SixTango's Easy Liquid Culture Container Tek.
I like the use of baby bottles because of the ease and convenience of the nipple - c'mon, who doesn't love a good nipple?
Anyhow, here's what I did:
 Materials - evenflo 4oz glass baby bottle, filter fiber (i.e. polyfill ), glass marble, tubing, scissors.
 Snip nipple just below the tip.
 Push the tubing through bottom of nipple.
 Cut tubing.
 Insert filter material into tubing.
 Drop the marble in.
 Finished product.
Further information for those who don't already know:
Liquid culture is a method where spores, an agar wedge, or mushroom tissue is injected/inserted/placed into a nutritious solution and incubated, for the purpose of inducing mycelium growth, thereby creating a large amount of inoculum that can be used to inoculate cakes or spawn with. The primary benefits of doing this are as follows:
* Lots and lots ( and lots ) of innoculant can be easily created - 1 10cc multi-spore syringe can be turned into 10 jars of LC, and 100mls of LC can create 10 syringes... pretty cool.
* Using LC to inoculate cakes or spawn can vastly speed up colonization - no lag time waiting for spore germination, in addition to the large amount of inoculation points when injected into substrate.
* The LC can be stored over long periods - throw it in your fridge for months.
Following are the beneficial aspects of using a baby bottle as a container for your LC:
* The nipple serves as a self-healing injection point.
* The nipple can be bent over or squeezed just below the filter tube in order to prevent the LC from saturating the filter when shaking or when drawing up solution.
* Easy and quick to prepare - no need to drill or seal.
* Convenient size, and it's nice to have the ml's/oz's right on the bottle.
Other things, not unique to the baby bottle tek, but useful in any LC container:
* The marble is used as means to better stir/mix the LC, and to break up any mycelium clumps that may occur.
* The filter is for gas exchange.
-------------------- Nature is the Technology of the Divine.
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HippieChick
Chicks can do it too!


Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 12 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4146428 - 05/07/05 12:55 PM (18 years, 8 months ago) |
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Was wondering, could one use colonized WBS to make a LC? Peace,Love,Happiness and Harmony. Hippie Chick
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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VALIS
vast activelivingintelligencesystem


Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 11 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: HippieChick]
#4146466 - 05/07/05 01:00 PM (18 years, 8 months ago) |
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Quote:
HippieChick said: Was wondering, could one use colonized WBS to make a LC?
My guess is Yes - any live/viable shroom material/tissue/organic-matter you throw into the LC will cause mycellium to grow.
It's ok to have to have solid material in your LC.
-------------------- Nature is the Technology of the Divine.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4146557 - 05/07/05 01:13 PM (18 years, 8 months ago) |
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LOL...... In another life... I posted here as 6T. agar=SixTango.
Yup, you can use a colonized seed/grain to start an LC.
Just make sure the transfer is done in a CLEAN way.... with MINIMAL open air exposure............otherwise - contam farm.
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VALIS
vast activelivingintelligencesystem


Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 11 years, 8 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4146622 - 05/07/05 01:24 PM (18 years, 8 months ago) |
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Quote:
agar said: LOL...... In another life... I posted here as 6T. agar=SixTango
You should have mentioned that when I originally made the above post ... guess maybe you must have missed it.
People are funny.
-------------------- Nature is the Technology of the Divine.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4146838 - 05/07/05 02:00 PM (18 years, 8 months ago) |
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I did miss it. Excuse = very hung over today.
 To much FUN last night
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