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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Perlite, Vermiculite

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Offlinedivinityinfinity
Hot LittleMuffin

Registered: 04/15/05
Posts: 176
Last seen: 8 years, 1 month
oh noes.....mycelium quandries.
    #4139281 - 05/05/05 09:14 PM (11 years, 7 months ago)

Ok so im definatley first time and using the PF tek. I inoculated 10 jars 4 days ago and i am not seeing ANY signs of mycelium at all. Not even a speck, on any of the jars.

Im using vermiculite, brown rice flour, etc. Lysolled my kitchen, boiled them using two layers of tin foil for an hour and a half, inoculated them 2 1/2 hours later (too SOON!?!?) with gloves, duskmask and all, and placed them in my little styrofoam cooler incubator, keeping the temp around 80 degrees. Im so scared i have dead babies. I was really paranoid about contamination so i heated the needle after every 3 jars. It made a popping noise when i did it -- bad? Eugh.

If this fails i still have 3 cc's left, i was thinking about doing the method with perlite in a tub, but i cant find it anymore...


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InvisibleShnezbit
Psycho-naught
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Registered: 09/30/04
Posts: 1,196
Loc: The Threshold.
Re: oh noes.....mycelium quandries. [Re: divinityinfinity]
    #4139306 - 05/05/05 09:22 PM (11 years, 7 months ago)

hmm...sounds like you inoculated them too soon. maybe try re-inoculating.


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 1 day, 20 hours
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Re: oh noes.....mycelium quandries. [Re: Shnezbit]
    #4139326 - 05/05/05 09:29 PM (11 years, 7 months ago)

Could have been too soon. I know 2 1/2 hours after PC'ing would kiil them. Keep hoping.
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OfflineKalix
'Head

Registered: 03/20/05
Posts: 1,504
Last seen: 11 years, 6 days
Re: oh noes.....mycelium quandries. [Re: HippieChick]
    #4139328 - 05/05/05 09:31 PM (11 years, 7 months ago)

I took a nap after steaming, when I woke up I innoc'd my jars where warm to the touch, and when I broke the tape w/the needle, I heard popping. Within 3 days I had heavy mycelial growth. Good luck, have patience...


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Offlinedivinityinfinity
Hot LittleMuffin

Registered: 04/15/05
Posts: 176
Last seen: 8 years, 1 month
Re: oh noes.....mycelium quandries. [Re: Kalix]
    #4139478 - 05/05/05 10:07 PM (11 years, 7 months ago)

bah! if it isnt that, could it be that i packed the vermiculite too tightly? it seems to be fine grained as well. I would fill the half pint jars to the top with the mixture then pack it down a half an inch and fill the rest with dry vermiculite


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OfflineKalix
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Re: oh noes.....mycelium quandries. [Re: divinityinfinity]
    #4139541 - 05/05/05 10:19 PM (11 years, 7 months ago)

I didn't pack my verm @ all... I think loose is better for air circulation. This is my 1st attempt tho, so I'm not sure.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Perlite, Vermiculite

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