Preserving for future cloning.. - Advanced Mycology

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:

All-In-One Bags That Don't Suck

High THC Strains

Substrate Mix

Unfolding Nature: Being in the Implicate Order

Jump to first unread post

Pages: 1

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

uniboner
member

Registered: 07/19/01
Posts: 173
Loc: TO
Last seen: 9 years, 7 months

Preserving for future cloning..

#413610 - 10/04/01 11:25 AM (22 years, 6 months ago)

I need to stop growing for a few weeks as I will be moving to another place..

The thing is, I have some niiice ecuadors fruiting and there are a few beautiful specimens I wish to clone from in the future.
I have heard about the possibility of extracting some inner tissue and storing it in distilled water for future culturing.. will this work?
If so, aside from watching the temp of the water, what other factors may I need to take into account for this venture?

Any and all help will be appreicated.

peace and thanks,
uniboner

Without nipples, breasts would be pointless...

--------------------
sleep is for the weak.

Extras:

Top

blackout

Registered: 07/16/00
Posts: 5,266
Last seen: 5 months, 9 days

Re: Preserving for future cloning..

[Re: uniboner]
#413714 - 10/04/01 01:32 PM (22 years, 6 months ago)

i tried doing it recently. i had some cut up inner stalk in a jar of water and put a big piece on some agar. i got no growth though, but i may have made the agar too strong as in over 4% malt since it looks dark and i didnt bother measuring properly. ill try it agin in the next few days and keep u posted

Extras:

Top

Suntzu
Geek

Registered: 10/14/99
Posts: 1,396
Last seen: 14 days, 14 hours

Re: Preserving for future cloning..

[Re: uniboner]
#413719 - 10/04/01 01:36 PM (22 years, 6 months ago)

It could/should/theoretically be OK. . .more often this technique is done with agar growth, thus ensuring contam-free dH2O. Remember that if you get a tiny unseen bacteria or mold colony in your liquid inoculum, the subsequent expansion will be disastrous.

If your fruiting chamber is pretty clean, and your transfering technique is good, then putting a chunk [skillfully extracted] into dH2O will maintain the strain for a long time, esp. if frigidated [I think it was Anno that pointed out a reference claiming 2+ years without loss of vigor]. I can't speak for peroxide with such a tech, some fish antibiotics might help.

Extras:

Top

puscle
genius of love

Registered: 01/06/01
Posts: 4,539
Loc: NY

Re: Preserving for future cloning..

[Re: uniboner]
#413778 - 10/04/01 03:07 PM (22 years, 6 months ago)

This has worked for me. Nine parts H20 to one part (3%) H202.

Extras:

Top

felixhigh
Scientist

Registered: 06/24/01
Posts: 7,565
Loc: Ly
Last seen: 4 days, 15 hours

Re: Preserving for future cloning..

[Re: uniboner]
#414254 - 10/04/01 09:59 PM (22 years, 6 months ago)

you could put some stripes of inner stem matter in honeywater, by the time you have moved you'll have mycelium clouds floating around... try to avoid big pieces, they're likely to rot... thin stripe pieces will do the job... you've got time.

i hate witty sigs / if anything i say has something in common with reality, this is a merely a coincidence.

Extras:

Top

uniboner
member

Registered: 07/19/01
Posts: 173
Loc: TO
Last seen: 9 years, 7 months

Re: Preserving for future cloning..

[Re: felixhigh]
#414859 - 10/05/01 12:47 PM (22 years, 6 months ago)

Thank you all for your help.
I would be a much more frustrated amatuer mycologist without your tips.

thanks and peace,
uniboner

Without nipples, breasts would be pointless...

--------------------
sleep is for the weak.

Extras:

Top

Jump to top

Pages: 1

Shop:

All-In-One Bags That Don't Suck

High THC Strains

Substrate Mix

Unfolding Nature: Being in the Implicate Order

Mushrooms, Mycology and Psychedelics > Advanced Mycology

Similar Threads	Poster	Views	Replies	Last post
Mexicana Strain A Scelortia Cloning Question.	bent	3,590	9	06/08/04 02:19 AM by RogerRabbit
Multispore vs. clone & woodlovers question	altarego	1,468	5	02/09/02 06:22 PM by altarego
5 questions on cloning	blackout	2,812	6	08/21/01 11:16 PM by jonnyshaggs420
making liquid inoculum	pluteus	3,110	11	12/15/03 06:54 AM by micro
Clone from cloned mycelium...	ATWAR	1,463	2	05/07/04 09:53 PM by ATWAR
Cardboard cloning tek? ( 1 2 all )	ink	26,081	31	02/14/16 10:43 PM by Eywa_devotee
Cloning PE For Spores	NESpores	2,794	13	03/01/06 11:00 PM by shymanta
Dried fruit cloning?	JDPhish	2,834	4	11/08/01 05:48 PM by anima

Extra information

You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
854 topic views. 0 members, 5 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]