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 liquidplaya
member
Registered: 06/07/00
Posts: 124
Last seen: 22 years, 10 months
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 wierd rye contam - endospores - bacteria - presoak 
#412601 - 10/03/01 02:48 PM (23 years, 9 days ago) |
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Hi
-preboiled rye for an hour
-pcooked for an hour at 15 psi
-some 250 ml jars, some 500 ml jars
-all jars show myceliual growth..
-jars were shaken, jars left for 3 more days
but no growth in 500 ml jars, half of 250 ml jars finished colonizing.. other half smell funky.. and all 500 ml jars smell funky.. and the funky smelling ones, the grain turned mushy.. while the colonized look drier..
any idea what happened? Endospores perhaps.. I haev a crappy Pcooker that might not pcook the jars properly, thus the 250 ml jars partially made it, but 500 ml was not pcooked enuff?
I am very sad..
Many many jars lost.
PLease help.
Edited by liquidplaya on 10/03/01 08:50 PM.
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 puscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
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Re: wierd ryte contam [Re: liquidplaya]
#412608 - 10/03/01 02:53 PM (23 years, 9 days ago) |
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That's bacteria. It could have been your inoculate or your sterilizing.
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liquidplaya
member
Registered: 06/07/00
Posts: 124
Last seen: 22 years, 10 months
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Re: wierd ryte contam [Re: puscle]
#412617 - 10/03/01 03:02 PM (23 years, 9 days ago) |
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so this could be the endospore problem right?
I preboiled
but now I think I will try boiling and soaking for 10 hours..
will this make a difference?
I am verrrry sadddddd and dont know what to do
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Mordor
cyborg savage
Registered: 05/15/01
Posts: 118
Loc: North
Last seen: 21 years, 1 month
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Re: wierd ryte contam [Re: liquidplaya]
#412621 - 10/03/01 03:09 PM (23 years, 9 days ago) |
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This is not an endospore problem. You killed them. Endospores produce a green mold and is easily detectable. You may have too much liquid in the jars. Be sure to strain your rye thoroughly and do not inject too much spore solution in small jars - it throws off the ratio and will turn the rye mushy.
It does sound like it may be your syringes.
On a side note, I never shake the jars. Fill to 1/2" from top with rye and top that with verm. Don't shake next time for a test.
I plan on using bags soon. This should eliminate a lot of hassles. Good luck.
It's life. The dark that binds you. - InFlames.
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It's life. The dark that binds you. - InFlames.
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liquidplaya
member
Registered: 06/07/00
Posts: 124
Last seen: 22 years, 10 months
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Re: wierd ryte contam [Re: Mordor]
#412628 - 10/03/01 03:23 PM (23 years, 9 days ago) |
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I read in a previous post that green mold is NOT produced by endospores. And that endospores produce bacteria which do not grow mold.
The jars seem to not have any excess liquid.. but after i shook them, it seems like there suddenly appeared more liquid...
how could not shaking stop contams.. since shaking does not add any contams since the jars are tight when I shake them.
I think I will persoak for 12 hours..
no precook
and then make sure I drain it good.. to make SURE its not a water problem..
I dont think it could be a syringe problem.. I made 6 syringes separately.. at different times.. using oven tek and lysol spray and alcohol..
how could they all contam with the same bacteria.. i would have had some type of colors and shiet if the area was contaminated..
Edited by liquidplaya on 10/03/01 04:32 PM.
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liquidplaya
member
Registered: 06/07/00
Posts: 124
Last seen: 22 years, 10 months
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Re: wierd ryte contam [Re: liquidplaya]
#412642 - 10/03/01 03:45 PM (23 years, 9 days ago) |
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I found an excellent post on this after an hour of searching..
http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum2&Number=278826&Forum=All_Forums&Words=rye&Match=Entire%20Phrase&Searchpage=9&Limit=50&Old=1year&Main=278826
I think I will listen to these ppl, as they have had the exact same problem I have had and have fixed it by soaking overnight in water.. I will try this
and leave a control jar to see if it is indeed my innoculation technique or if it is the endospores.
I will keep ya'll updated.
half of the 250 ml jars I think I left out for a while before p-cooking too.. so perhaps this is it!
I will be praying.. cause I Dont wanna be sittin here cryin over my jars again in a few weeks...
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oscill8
*bondage fairy*
Registered: 02/26/01
Posts: 1,215
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Re: wierd ryte contam [Re: liquidplaya]
#412644 - 10/03/01 03:47 PM (23 years, 9 days ago) |
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wet spot?
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? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"
Edited by oscill8 (11/15/01 11:14 PM)
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liquidplaya
member
Registered: 06/07/00
Posts: 124
Last seen: 22 years, 10 months
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Re: wierd ryte contam [Re: oscill8]
#412660 - 10/03/01 04:03 PM (23 years, 9 days ago) |
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see thats the thing. i did use some spores. some culture transfers...
and all these contamed..
well if you aer having the problem with all your jars and your jars are made using soaked rye.. then what the hell coujld the problem be..
are you sure you are using soaked for 12 hour rye?
I will also drain them even more to make suer the water content is minimal..
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Champion des Champignons
long standing member;)
Registered: 07/26/00
Posts: 2,680
Loc: Alba
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Re: wierd ryte contam [Re: liquidplaya]
#413155 - 10/04/01 12:26 AM (23 years, 9 days ago) |
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I've said it before, and it looks like I'll say it again:D
Precook the grains until they're soft, but not squishy (a good way to judge this is when a few grains have burst open), pack into whatver type jars you are using. Then leave at room temp for _at least_ 24 hours. This should be enough time for any endospores to germinate, and thus become susceptible to heat. Then p/c for 45 mins at 15+ psi. If you're really paranoid you can p/c the day after precooking for 20 mins or so, then p/c for 45 mins the day after. If this doesn't stop your wetspot probs then the prob is most likely the innoculant.
Lies! All lies!
Support the FSR!
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hmmm........
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liquidplaya
member
Registered: 06/07/00
Posts: 124
Last seen: 22 years, 10 months
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im doin this as we speak
and will test if its the innoculant by keeping a jar without innoculation and Oxygen diffusion by keeping the jar tight and not innoculate it..
so we will see what is causing the contam ie: endospores, innoculant contam.
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NDK
member
Registered: 07/13/01
Posts: 186
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Re: wierd ryte contam [Re: liquidplaya]
#413341 - 10/04/01 05:26 AM (23 years, 8 days ago) |
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Listen to Champignion - pre-boiling sorts the moisture levels and the endospores
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liquidplaya
member
Registered: 06/07/00
Posts: 124
Last seen: 22 years, 10 months
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Re: wierd ryte contam [Re: NDK]
#413646 - 10/04/01 12:22 PM (23 years, 8 days ago) |
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UPDATE:
- purchased 22 qt mirro pressure cooker
- soaked rye for 24 hours
- pressure cooked for 1 hour
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