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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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Amazon Shop for: ½ Pint Jars, Agar

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Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
So tonight i did a lazy style inoculation tek.
    #4126007 - 05/03/05 01:27 AM (11 years, 5 months ago)

I was about 100% sloppier tonight than i usually am. I used a glove box but i didn't sterilize the shit out of the inside with lysol. I usually rub the jars before putting them in the glove box with 70% isopropyl.

I inoculated nine 1 litre jars with finch seed. Liquid culture.
EDIT: (1 cup finch seed to 65 ml water, PC 121C, 90 min./first time using this recipe of my own devising.)
I usually chop up colonized agar in a sterile jar with water, followed by drawing it up in a syringe. WHen i went to screw the cap for the inoculant jar, it wouldn't go back on tight, so when i shook it to break up mycelium, the water was shooting out from under the cap.

This is super sloppy compared to what i usually do. I'm usually super meticulous and get no jar contams. I'm curious to see whether i can get away with it, because my normal method takes too damn long.

I hope it works.

If thi doesn't make sense, my apologies, i'm baked.

Man thinks. God laughs. - Jewish Proverb

Edited by no-tone (05/03/05 01:31 AM)

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Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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