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OfflineWorkmanV
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Selection of "Psilocybe atlantis"
    #4123889 - 05/02/05 03:20 PM (18 years, 8 months ago)

Small test fruitings of a relatively new undescribed species from Georgia. According to Guzman, this is actually not Psilocybe atlantis but yet another species which was inadvertently collected along with Psilocybe atlantis samples. Unfortunately this has caused some confusion and circulating strains of "Psilocybe atlantis" are entirely this yet unnamed species.

I was sent a a pair of slides containing about a dozen spore prints of this species several years ago. I recently selected 7 prints and streaked them on agar. One grew sluggishly and was rejected and another got overlooked when I inoculated the grass seed and will be tested at a later date. Out of the 5 remaining cultures 3 are fruiting well.


Best performers

The winner so far. It quickly forms sclerotia on grass seed invitro and fruited the earliest out of the 5.


All fruiting mini-trays were inoculated with multispore cultures and several strains may be showing in any one tray. Many are growing long and bendy but some are more robust. The best specimen of mushroom and sclerotia will be cloned and fruited as a true isolation.


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Edited by Workman (05/02/05 03:31 PM)


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OfflineWorkmanV
1999 Spore War Veteran
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Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 14 hours, 29 minutes
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Re: Selection of "Psilocybe atlantis" [Re: EonTan]
    #4127796 - 05/03/05 01:00 PM (18 years, 8 months ago)

I am afraid I won't be releasing anything new until my issues with a parasitic vendor are dealt with.  Sorry :frown:


--------------------
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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: blackout]
    #4153516 - 05/09/05 01:42 PM (18 years, 8 months ago)



Specimen number 7. A late developer, good form but only modest spore production. Specimen 3 has better spore production and is now producing stouter mushrooms as well as good sclerotia production. It is too early to tell how it compares to Mexicana A in potency and productivity. Further testing is scheduled.


--------------------
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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: Workman]
    #4161996 - 05/11/05 01:38 PM (18 years, 8 months ago)

Specimen number 3 second flush. Better spore production than most mexicana strains and promising sclerotia production in this particular strain.





Sclerotium forming.


--------------------
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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: Workman]
    #4260810 - 06/05/05 05:58 PM (18 years, 7 months ago)



Over mature specimen of sample #1. This mushroom grew suprisingly large, about 1.5 inches in diameter.


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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: Workman]
    #4291491 - 06/13/05 02:05 PM (18 years, 7 months ago)





Hole in left hand piece is from sample removed for cloning. The texture is somewhat different than what is seen in mexicana/tampanensis sclerotia. It seems more granular and uniform in appearance.


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Edited by Workman (06/21/05 01:25 PM)


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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: Workman]
    #4320781 - 06/21/05 01:19 PM (18 years, 7 months ago)

Specimen #1 again. This strain was rejected earlier because it was thought to be a poor spore producer. Actually it is a very good spore producer and the mushrooms were just being harvested too early. This strain continues to grow after the cap goes plane and only after becoming quite large does spore production begin. Clones of this specimen and specimen #3 which shows good sclerotia production will be put into new trials for production. Only after extensive testing are new strains/species released.



--------------------
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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: Shdwstr]
    #4320822 - 06/21/05 01:29 PM (18 years, 7 months ago)

Exactly, but probably not those exact names. The mexicana strains were not really named but just given their lab designation codes. This wasn't a well thought out choice but now that it is stuck it can't really be changed without undue confusion.


--------------------
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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: blackout]
    #4320939 - 06/21/05 02:05 PM (18 years, 7 months ago)

Far too early to say. Formation of small sclerotia was fairly rapid though, at least as fast as the fastest mexicana.


--------------------
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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: Workman]
    #4351323 - 06/29/05 03:50 PM (18 years, 6 months ago)

Before the mini test trays were destroyed, the grass seed was broken apart to check for sclerotia. Unfortunately, tray #2 was destroyed before it was checked. Still, there are some interesting results with the remaining four trays.



ATL #7 stands out with much larger but fewer sclerotia. Even though the total yield is lower, the ease of harvesting a few large and impressive stones is a worthwhile trade-off. This wasn't a scientifically designed test of sclerotia production and too much shouldn't be read into these results. The weights don't take into account mushroom production and the grass seed in each tray wasn't carefully measured.

In contrast to P. mexicana and P. tampanensis this species forms sclerotia entirely inside the substrate. P. mexicana and P. tampanensis tend to form the majority of their sclerotia between the glass-substrate interface or in the casing. "P. atlantis" consistantly hides its sclerotia deep within the substrate and they only become visible when they incidently grow up to the glass.



This image is a cut away view of a grass seed "cake" showing the sclerotia.

Close up of sclerotia from ATL #7. The largest two stones weigh 6.0 and 5.0 grams.



Out of the 5 strains, the three below will be tested further.

ATL #1 Largest fruits with good spore production. Decent sclerotia yield but many tiny stones.

ATL #3 Fastest fruiting and sclerotia formation, small fruits but good spore production. Highest total sclerotia yield but many tiny stones that make harvesting tedious.

ATL #7 Small fruits that develop late, moderate spore production. Largest sclerotia, very few small stones, no tiny stones.

update 8/17/05: I found a portion of the ATL#2 tray contents in the compost pile several weeks ago. It only contained the small sized sclerotia and didn't seem exceptional in any way.


--------------------
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Edited by Workman (08/17/05 01:39 PM)


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OfflineWorkmanV
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Re: Selection of "Psilocybe atlantis" [Re: blackout]
    #4372877 - 07/05/05 04:39 PM (18 years, 6 months ago)

I don't have any quantitative method of measuring psilocybin or psilocin levels.

I think that the mycelium forms the sclerotia at the point it runs out of room and/or food. This makes sense with morels, tamps and mexicana, but it doesn't explain why "atlantis" behaves differently.

I don't think adding more barriers to the jar in the form of glass or plastic plates will increase sclerotial volume. I would expect that you would just get more smaller stones instead of the large ones that form on the glass. I think that the total volume of the sclerotia can't be improved much, but I am not certain.

I do agreed that supplimental watering is likely to increase total sclerotial yield. Overly dry substrates produce stones poorly. You might try adding some rice to a grass seed mix since it holds a great deal more water than just pure grass seed alone.

20C = 68F. These were being fruited at 25C = 78F and were not intended for sclerotia production. They may produce differently at lower temperatures.


--------------------
Research funded by the patrons of
The Spore Works
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My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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OfflineWorkmanV
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Registered: 03/01/01
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Re: Selection of "Psilocybe atlantis" [Re: Workman]
    #4500015 - 08/05/05 01:08 PM (18 years, 5 months ago)

Strain ATL#7 should be released by early next week. I expect that strain ATL#1 will be available later this month.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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OfflineWorkmanV
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Registered: 03/01/01
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Re: Selection of "Psilocybe atlantis" [Re: stvenkman]
    #4567231 - 08/22/05 04:57 PM (18 years, 5 months ago)

The containers are exactly one cup (Gladware brand).

I haven't tried the addition of rice myself but it was mentioned in Gartz's book "Magic Mushrooms Around the World"


--------------------
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My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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